CARBOHYDRATE POLYMERS
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2023
·
IF 11.2
Shengchang Tao,Yuchen Song,Shaobo Ding,Ruirong He,Qiankun Shi,Fang Hu
Photodynamic therapy (PDT) eradicates tumors via the generation of toxic reactive oxygen species (ROS) by activation of a photosensitizer (PS) with appropriate light. Local PDT toward tumors can trigger the immune response to inhibit distant tumors, but the immune response is usually insufficient. Herein, we used a biocompatible herb polysaccharide with immunomodulatory activity as the carrier of PS to enhance the immune inhibition of tumors after PDT. The Dendrobium officinale polysaccharide (DOP) is modified with hydrophobic cholesterol to serve as an amphiphilic carrier. The DOP itself can promote dendritic cell (DC) maturation. Meanwhile, TPA-3BCP are designed to be cationic aggregation-induced emission PS. The structure of one electron-donor linking to three electron-acceptors endows TPA-3BCP with high efficiency to produce ROS upon light irradiation . And the nanoparticles are designed with positively charged surfaces to capture antigens released after PDT, which can protect the antigens from degradation and improve the antigen-uptake efficiency by DCs. The combination of DOP-induced DC maturation and antigen capture-increased antigen-uptake efficiency by DCs significantly improves the immune response after DOP-based carrier-mediated PDT. Since DOP is extracted from the medicinal and edible Dendrobium officinale , the DOP-based carrier we designed is promising to be developed for enhanced photodynamic immunotherapy in clinic.
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BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2022
·
IF 1.462
Xue-Feng Xu,De-Cai Dai,Hao Yan,Yu Zhang
The phytochemical investigation of the stems of Dendrobium officinale Kimura et Migo led to the isolation of a new chromone , chromcinale ( 1 ) and a new phenolic glycoside , leonuriside C ( 5 ), together with three known biogenetically related phenanthrenes ( 2 – 4 ), three known biogenetically related phenolic glycosides ( 6 – 8 ) and four known biogenetically related phenylpropanoids ( 9 – 12 ). The structures of these compounds were elucidated using comprehensive spectroscopic methods . Compounds 1–3 , 5–12 were isolated from the D. officinale for the first time. The chemotaxonomic significance of the isolates was also discussed.
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Frontiers in Nutrition
·
2022
·
IF 6.59
Liu Ruimin,Shi Songshan,Xiong Si,Su Juan,Gan Xiaona,Wu Jianjun,Wang Huijun,Wang Shunchun
Dendrobium officinale Kimura et Migo has been used as a traditional Chinese medicine (TCM) and a functional food for thousands of years. Carbohydrate is one of the most important effective substances and indicative components in D. officinale. However, since the qualitative and quantitative analysis of polysaccharides in D. officinale remains a challenge and limitation, herein, an oligosaccharide-quality marker approach was newly developed for quality assessment of D. officinale by spectrum–effect relationships between high performance liquid chromatographic (HPLC) fingerprints and anti-inflammatory effects. The HPLC fingerprints of 48 batches of oligosaccharides from D. officinale (DOOS) were developed and analyzed with similarity analysis (SA) and hierarchical cluster analysis (HCA), and eight common peaks were identified. In vitro screening experiment indicated that DOOS potentially inhibited nitric oxide (NO) production and effectively reduced the release of inflammatory cytokines, such as TNF-α, IL-6, and IL-1β in RAW 264.7 cells, thereby reducing the inflammatory response of cells. Finally, the HPLC fingerprint of different batches of DOOS was combined with in vitro anti-inflammatory activity to assess the spectrum–effect relationships of DOOS by gray correlation analysis (GCA), in addition, the purified oligosaccharide components were identified and validated for NO inhibitory activity. Our results showed four DOOS (maltotetraose, maltopentaose, maltohexaose, and mannohexaose) were relevant to anti-inflammatory effects and could be as quality markers for the quality control of D. officinale. It suggests that the “oligosaccharide-spectrum-effect” relationships approach is a simple and reliable method for the quality control of herb medicines or nutritious foods.
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CARBOHYDRATE RESEARCH
·
2026
·
IF 3.4
Xingchen Wang,Mingzhu Cao
An extraction-purification process for Dendrobium officinale polysaccharides was established, with a yield of 35.03%, content of 49.34%, and protein removal rate of 78.70%. Two polysaccharides, DOP‑1 (linear) and DOP‑2 (branched), protect against photoaging via the TGF‑β/Smad pathway, with DOP‑2 showing stronger activity. Objective: To optimize the extraction-purification process of Dendrobium officinale polysaccharides (DOPs) and characterize the fine structures of two homogeneous fractions (DOP-1, DOP-2), as well as to evaluate their anti-photoaging activity on HFF cells.Methods: An integrated process including petroleum ether defatting, cellulase hydrolysis, water extraction-ethanol precipitation, trypsin-Sevag deproteinization, activated carbon decolorization, and dialysis was used. DOP-1 and DOP-2 were separated by DEAE-52 and Sephadex G-100 chromatography. Their structures were analyzed by FT-IR, methylation-GC/MS, NMR, and SEM. Anti-photoaging effects were assessed via CCK-8, SA-β-Gal staining, and qPCR of TGF-β/Smad pathway genes.Results: The optimized process increased crude polysaccharide yield from 30.34% to 35.03% and polysaccharide content from 31.23% to 49.34%. DOP-1 (Mn 989 Da, PDI 1.15) was a linear oligosaccharide composed of →4)-β-D-Manp (72.32%) and →4)-β-D-Glcp (23.78%) in an alternating pattern. DOP-2 (Mn 2970 Da, PDI 1.67) was a branched polysaccharide with a backbone of →6)-α-D-Glcp (58.06%) and branches at →2,6)-Manp and →4,6)-Glcp. Both DOP-1 and DOP-2 (62.5 μg/mL) protected HFF cells from UVA-induced photoaging, upregulated TGF-β1, Smad3, Smad4, and Collagen I, and downregulated Smad7 and MMP-1, with DOP-2 showing dose-dependent effects.Conclusion: The optimized process enables efficient preparation of DOPs. DOP-1 and DOP-2 possess distinct structural features and exert anti-photoaging effects via regulation of the TGF-β/Smad signaling pathway. Download: Download high-res image (364KB) Download: Download full-size image
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FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Haihong Chen,Qixing Nie,Jielun Hu,Xiaojun Huang,Wenqi Huang,Shaoping Nie
Diabetes is a metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion and/or action. The anti-diabetic effects of polysaccharide from Dendrobium officinale stem (DOP) on type II diabetic rats was evaluated in this study. Treatment with DOP significantly decreased the concentrations of fasting blood glucose, insulin, glycated serum protein, serum lipids (TC, TG, LDL-c, NEFA), and increased the concentration of HDL-c, compared with the untreated diabetic rats. In addition, DOP treatment alleviated the oxidative stress injury by increasing the activity of superoxide dismutase and total antioxidant capacity , as well as decreased the level of malonaldehyde. Histopathological analysis revealed that DOP has protective effects on type II diabetes induced pancreatic injury. Serum metabolomics and lipidomics analysis also found DOP treatment could decrease serum fatty acids, glycerolipids, sphingolipids, phospholipids and bile acids, and alleviate the metabolism disorders of lipid, bile acids and amino acids .
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CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Lian He,Xiaoteng Yan,Jian Liang,Shijie Li,Hongrui He,Qingping Xiong,Xiaoping Lai,Shaozhen Hou,Song Huang
The purpose of this study was to screen the optimum extraction of polysaccharides (DOP) from Dendrobium officinale stem. Firstly, different methods, including hot water extraction (HWE), cold-pressing (CP), freeze-thawing cold-pressing (FTCP), ultrasonic-assisted hot water extraction (UHWE), microwave-assisted hot water extraction (MHWE) and enzyme-assisted hot water extraction (EHWE), were employed to extract DOP under their respective best parameters. Then, the extraction yield, structure and antioxidant activity of the polysaccharides from different extraction methods were compared under the same condition. The data implied that UHWE and FTCP possessed higher extraction yield than the other extraction methods. Besides, DOP CP and DOP FTCP had higher molecular weight than the other polysaccharide samples. More importantly, DOP FTCP had the highest antioxidant activity. Overall, DOP FTCP exhibit high extraction yield, well-preserved molecular chains and best antioxidant activity, all these indicated FTCP was the most suitable method to extract DOP.
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Journal of Cosmetic Dermatology
·
2024
·
IF 2.3
Xing-Li-Shang He,Ning Wang,Xi Teng,Nan-Nan Wang,Zhi-Yi Xie,Ying-Jie Dong,Min-Qiu Lin,Ze-Hua Zhang,Mei Rong,Yi-Gong Chen,Bo Li,Gui-Yuan Lv,Su-Hong Chen
Background Dendrobium officinale flowers (DOF) have the effects of antiaging and nourishing yin, but it lacks pharmacological research on skin aging. Objective Confirming the role of DOF in delaying skin aging based on the “in vitro animal–human” model. Methods In this experiment, three kinds of free radical scavenging experiments in vitro, D-galactose-induced aging mouse model, and human antiaging efficacy test were used to test whether DOF can improve skin aging through anti-oxidation. Results In vitro experiment shows that DOF has certain scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, hydroxyl free radical, and superoxide free radical, and its IC 50 is 0.2090 μg/mL, 15.020, and 1.217 mg/mL respectively. DOF can enhance the activities of T-AOC, SOD, CAT, and GSH Px in the serum of aging mice, increase the content of GSH, and reduce the content of MDA when administered with DOF of 1.0, 2.0, and 4.0 g/kg for 6 weeks. In addition, it can enhance the activity of SOD in the skin of aging mice, increase the content of Hyp, and decrease the content of MDA, activated Keap1/Nrf2 pathway in the skin of aging mice. Applying DOF with a concentration of 0.2 g/mL on the face for 8 weeks can significantly improve the skin water score and elasticity value, reduce facial wrinkles, pores, acne, and UV spots, and improve the facial brown spots and roughness. Conclusion DOF can significantly improve skin aging caused by oxidative stress, and its mechanism may be related to scavenging free radicals in the body and improving skin quality.
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CHEMISTRY OF NATURAL COMPOUNDS
·
2023
·Vol. 59
·期 6
·1067-1074
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IF 0.8
Chen Yi-Fang,Luo Ji-Feng,Zhang Yan-Ni,Wang Yue-Hu
Four flavonoid C -glycosides, including two new diglycosides, dendrodiglycosides A ( 1 ) and B ( 2 ), along with known isoviolanthin ( 3 ) and apigenin 6- C - β -D-glucopyranosyl-(1→2)-α-L-arabinopyranoside ( 4 ), were isolated from the leaves of Dendrobium officinale (Orchidaceae). Their structures were elucidated by NMR and MS. Apigenin 6- C - β -D-glucopyranosyl-(1→2)-α-L-arabinopyranoside exhibited an inhibitory effect against tyrosinase with an IC 50 value of 146.5 μg/mL.
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Food Bioscience
·
2025
·
IF 5.9
Yan Long,Jiajing Yang,Hongfei Ji,Yuting Fan,Keyao Dai,Wenjie Ding,Haiyu Ji,Juan Yu
Dendrobium officinale polysaccharides (DOP), as a natural active ingredient, has exhibited strong immunomodulatory and antioxidant effects. Selenium nanoparticles (SeNPs) also possess various biological activities, but the unstable properties limit their applications. This research focused on modification of SeNPs by DOP to form stable complexes (DOP-SeNPs), and the analysis of structural characterization and gut microbiota-mediated immune regulation mechanisms. The results showed that DOP-SeNPs were prepared through hydrogen bond interaction and presented uniform particle size distribution (average particle size of about 100 nm) and good stability. Animal experiments demonstrated that DOP-SeNPs significantly protected the immune organs in cyclophosphamide-induced immune injury mice. Besides, DOP-SeNPs effectively regulated the gut microbiota diversity including promoting the enrichments of probiotics such as Lactobacillus and Lachnospiraceae NK4Al36 group compared with model group, and improved the lymphocyte subsets proportions through the regulation of metabolic pathways including biosynthesis of various antibiotics, protein digestion and absorption, chemical carcinogenesis-DNA adducts. In conclusion, DOP-SeNPs presented stable structure characteristics and improved the immune functions of immune-injured mice by influencing the gut microbiota metabolism. These results will provide a certain theoretical basis and data support for the applications of DOP and the nano-selenium complex in functional food industry.
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FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Bo Wang,Weimin Zhang,Xinpeng Bai,Congfa Li,Dong Xiang
The physicochemical and rheological properties of polysaccharides extracted from stems of Dendrobium officinale (DOP) were investigated. Two samples were prepared: the first sample (HEPDOP) was prepared by pretreating D. officinale stems by hot water scalding, followed by water extraction and ethanol precipitation; and the second sample (EPDOP) was obtained by water extraction and ethanol precipitation without pretreatment. The contents of total carbohydrate and protein, mannanase activity, infrared spectra, molecular weight distribution, thermal properties, and hydrophobicity of the two samples were analyzed. The results showed that mannanase activity of HEPDOP (0.0247 U) was much lower than that of EPDOP (0.1158 U). HEPDOP had higher molecular weight and carbohydrate content, and lower polydispersity index (PDI) value and protein content compared to EPDOP. The results further showed that the decomposition temperature of HEPDOP was 311 °C, which is higher than that of EPDOP (306 °C); and its hydrophobicity was significantly lower than that of EPDOP. Interestingly, at the same concentration (2% (w/w)), HEPDOP had higher viscosity than EPDOP, and acidity, alkalinity, the addition of salt ions, and temperature was found to reduce their viscosity. Finally, we observed that increasing temperature caused the decrease of viscosity and modulus of both DOPs.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 21
·期 1
·54
·
IF 4.183
Lei Zhang,Chunyan Jiao,Yunpeng Cao,Xi Cheng,Jian Wang,Qing Jin,Yongping Cai
Studies have shown that the type II pyridoxal phosphate-dependent decarboxylase (PLP_deC) genes produce secondary metabolites and flavor volatiles in plants, and TDC (tryptophan decarboxylase), a member of thePLP_deCfamily, plays an important role in the biosynthesis of terpenoid indole alkaloids (TIAs). In this study, we identified eightPLP_deCgenes inDendrobium officinale(D. officinale) and six inPhalaenopsis equestris(P. equestris), and their structures, physicochemical properties, response elements, evolutionary relationships, and expression patterns were preliminarily predicted and analyzed. The results showed thatPLP_deCgenes play important roles inD. officinaleand respond to different exogenous hormone treatments; additionally, the results support the selection of appropriate candidates for further functional characterization ofPLP_deCgenes inD. officinale.Keywords:Dendrobium officinale;PLP_deC;bioinformatics;expression pattern analysis;evolution
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MOLECULES
·
2018
·Vol. 23
·期 4
·818
·
IF 3.098
Siyan Zheng,Yingpeng Zhu,Chunyan Jiao,Mengyao Shi,Lianping Wei,Yang Zhou,Qing Jin,Yongping Cai
In order to optimize the extraction of gigantol fromDendrobium officinale, the influence of methanol concentration, ultrasonic temperature, and liquid ratio on extraction efficiency was analysed by the response surface analysis method. The results show that the extraction rate reached a maximum when the methanol concentration was 92.98%, the solid-liquid ratio was 27.2 mL/g, and the extraction temperature was 41.41 °C. The content of gigantol ofDendrobium officinalein leaves was significantly higher than that in stems, reaching 4.7942 μg/g. The content of gigantol inDendrobium huoshanensisFengdou was significantly higher than that of other species of Fengdou. This experiment has practical significance for improving the utilization rate ofDendrobium officinale, and provides a reference for the study of the pharmacological and biological activity of gigantol.Keywords:Dendrobium officinale;gigantol;response surface methodology;ultrasonic-assisted extraction
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