AQUATIC TOXICOLOGY
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2025
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IF 4.3
Shengjin Zhang,Mengting Wu,Xiao Wu,Yayun Yu,Hua Fang,Yunlong Yu,Luqing Zhang
The extensive application of silver nanoparticles (AgNPs) has resulted in their significant release into aquatic environments, leading to a high likelihood of co-occurrence with other contaminants. Among these, pesticides represent one of the most prevalent groups in aquatic systems. However, the combined effects of AgNPs and pesticides on aquatic organisms, especially benthic species, remain poorly understood. This study comparatively assessed the joint toxicity of AgNPs and clothianidin (CLO), a widely used neonicotinoid insecticide, on Limnodrilus hoffmeisteri under 24-h acute exposure in a water-only system and 14-d subacute exposure in a sediment-water system. The results demonstrated that AgNPs and CLO demonstrated synergistic lethality toward L. hoffmeisteri under acute exposure, partially attributed to CLO-facilitated dissolution of AgNPs and exacerbated oxidative stress upon co-exposure. Transcriptomic analysis further revealed that CLO exposure upregulated the genes associated with cellular respiration and protein synthesis/degradation; however, these adaptive responses were suppressed by the presence of AgNPs, thereby weakening the worms’ tolerance to CLO. Under subacute exposure, AgNPs alone exhibited negligible toxicity but enhanced CLO bioaccumulation in the worms, resulting in intensified oxidative stress and ultimately aggravating CLO-induced growth inhibition and neurotoxicity in L. hoffmeisteri . These results collectively revealed that AgNPs could synergistically amplify the toxicity of CLO toward L. hoffmeisteri in both water-only and water-sediment systems. The findings of this work contribute to a comprehensive understanding of the ecological risks associated with the co-occurrence of AgNPs and pesticides.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2013
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IF 2.906
Ming Li,Enze Sheng,Lujing Cong,Minghua Wang
Two enzyme-linked immunosorbent assays (ELISAs) based on polyclonal antibodies (PcAbs) for clothianidin are described: colorimetric detection format (ELISA) and pattern of chemiluminescent assay (CLEIA). Clothianidin hapten was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) to produce immunogen and coating antigen. Anticlothianidin PcAbs were obtained from immunized New Zealand white rabbits. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC20) of clothianidin were 0.046 and 0.0028 mg/L for the ELISA and 0.015 and 0.0014 mg/L for the CLEIA, respectively. There were no obvious cross-reactivities of the antibodies with its analogues except for dinotefuran. Recoveries of 76.4–116.4% for the immunoassays were achieved from spiked samples. The results of immunoassays for the spiked and authentic samples were largely consistent with gas chromatography. Therefore, the proposed immunoassays would be convenient and satisfactory analytical methods for the monitoring of clothianidin in agricultural products.
研究领域:
分析化学
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环境科学
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食品安全
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免疫学
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农药残留监测
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2014
·Vol. 21
·期 9
·5803-5809
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IF 2.757
Li Ming,Sheng Enze,Yuan Yulong,Liu Xiaofeng,Hua Xiude,Wang Minghua
Europium (Eu 3+ )-labeled antibody was used as a fluorescent label to develop a highly sensitive time-resolved fluoroimmunoassay (TRFIA) for determination of clothianidin residues in agricultural samples. Toward this goal, the Eu 3+ -labeled polyclonal antibody and goat anti-rabbit antibody were prepared for developing and evaluating direct competitive TRFIA (dc-TRFIA) and indirect competitive TRFIA (ic-TRFIA). Under optimal conditions, the half-maximal inhibition concentration (IC 50 ) and the limit of detection (LOD, IC 10 ) of clothianidin were 9.20 and 0.0909 μg/L for the dc-TRFIA and 2.07 and 0.0220 μg/L for the ic-TRFIA, respectively. The ic-TRFIA has no obvious cross-reactivity with the analogues of clothianidin except for dinotefuran. The average recoveries of clothianidin from spiked water, soil, cabbage, and rice samples were estimated to range from 74.1 to 115.9 %, with relative standard deviations of 3.3 to 11.7 %. The results of TRFIA for the blind samples were largely consistent with gas chromatography ( R
2 = 0.9902). The optimized ic-TRFIA might become a sensitive and satisfactory analytical method for the quantitative monitoring of clothianidin residues in agricultural samples.
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MICROCHEMICAL JOURNAL
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2025
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IF 5.1
He Chen,Juan Zhou,Li An,Huan Ma,Meng Li,Haixia Lei,Lei Wang,Lufei Zheng,Zenglong Chen,Xujin Wu
Clothianidin are significant toxicity toward non-target organisms, underscoring the necessity for a simple and highly sensitive biosensor for on-site detection. Herein, a pH responsive biotin-tagged horseradish peroxidase (HRP)-encapsulated nanoliposome (HRP@liposome) was synthesized and applied to a colorimetric immunosensor as signal amplification generator. In the presence of clothianidin, it competes with coated antigens for binding to biotinylated antibodies, thereby reducing the number of HRP@liposome due to biotin-streptavidin-biotin interactions. Upon addition of substrate solution, the HRP@liposome are lysed, releasing a substantial amount of HRP, which catalyzes the oxidation of substrate. Under optimal conditions, the colorimetric immunosensor exhibited a 50 % inhibition concentration of 4.75 ng mL −1 , with a limit of detection of 0.24 ng mL −1 , and a linear range of 0.24–66.23 ng mL −1 , respectively. The average recoveries were 86.1 %–103.6 % in spiked environmental and food samples. Compared with conventional enzyme-linked immunosorbent assays, the proposed immunosensor demonstrates higher sensitivity. This research provides a promising reference for rapid assessment in environmental health and food safety.
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FOOD AND AGRICULTURAL IMMUNOLOGY
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2019
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IF 2.398
Hongliang Li,Baoguo Sun,Tian Chen
Clothianidin (CTA), third generation neonicotinoid insecticide that has been widely used for the long-term control of a wide variety of pests. Therefore, we developed immunochromatographic assay for the detection of CTA residues in cucumber and apple juice. The antigen HCTA-EDC-OVA and the goat anti-mouse IgG antibody were added to a nitrocellulose membrane as the test and control lines, respectively. Under optimal conditions, the cut-off limits of the semi-quantitative strip test for CTA were 1 ng/mL in 0.01 M PBS (pH 7.4), 4 ng/mL in cucumber juice, and 5 ng/mL in apple juice, and the results were obtained within 5 min. The immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of CTA residues in cucumber and apple juice.
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Stress Biology
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2025
·Vol. 5
·期 1
·1-17
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IF 5.8
Luo Zhi,Li Zhen-Fei,Lin Zhi-Yu,Fu Zhen-Qiang,Han Feng-Lu,Li Er-Chao
Clothianidin, a widely used neonicotinoid pesticide, poses potential ecological risks to aquatic ecosystems due to its unique mode of action and widespread environmental dispersal. This study investigates the toxic effects of clothianidin on Penaeus vannamei at different concentrations over 28 days. High concentrations of clothianidin significantly affected shrimp physiology, as evidenced by changes in survival rate and weight gain. Markers of oxidative stress, including decreased respiratory burst, reduced glutathione levels, and diminished antioxidant enzyme activities, indicated that clothianidin triggered oxidative stress responses in shrimp. Additionally, changes in lactate dehydrogenase, succinate dehydrogenase, and isocitrate dehydrogenase activities suggested disruptions in energy metabolism in the hepatopancreas. Analysis of the nervous system revealed significant disturbances in neural signaling, reflected by altered levels of acetylcholine, acetylcholinesterase, and dopamine. Transcriptomic analysis highlighted significant changes in gene expression and metabolic processes in the nervous system. This study demonstrates that clothianidin disrupts oxidative balance, energy metabolism, and neural signaling, affecting the growth of P. vannamei and providing valuable insights into its biochemical and transcriptomic toxicity in aquatic environments. Graphic
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JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Tingting Liu,Jian Luo,Shangke Liu,Tongtong Li,Haolin Li,Lingyan Zhang,Wei Mu,Nan Zou
Neonicotinoid insecticides have been widely used due to their excellent systemic activity and high insecticidal activity, but the problems of low utilization rate and environmental risk have attracted widespread attention. Controlled-release technology is an approach to realize the efficient utilization of pesticides and reduce environmental pressure. In this study, clothianidin (CLO) controlled-release granules ( [email protected] TA (tannic acid)/Fe (III)) were prepared with TA/Fe (III) coordination chelate as the coating material. These granules exhibited the core-shell structure with 500–1200 µm of particle size, and had obvious release performance and hydrolysis behavior of coating materials. Pot experiments by root application showed that the [email protected] /Fe (III) showed balanced and lasting control efficacy to broad bean aphids. The plants have a stronger capacity for absorption and enrichment and a higher utilization rate of CLO for [email protected] /Fe (III), than those for 10% suspension concentrate (SC). One of the hydrolysates of coating materials, TA, a polyphenolic antioxidant, could improve the bioaccumulation amount and alleviating the oxidative stress response of CLO in plants. Our study illustrates that the controlled-release granules base on TA have efficient controlled-release properties and free radical scavenging performance that may eventually be used as pesticide carriers and antioxidants in the field of plant protection.
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CHEMICO-BIOLOGICAL INTERACTIONS
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2024
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IF 4.7
Yingying Hu,Zhize Ye,Hualu Wu,Xiaohai Chen,Hailun Xia,Jian-ping Cai,Guo-xin Hu,Ren-ai Xu
Clothianidin , classified as a second-generation neonicotinoid , has achieved extensive application due to its high efficacy against insect pests . This broad-spectrum usage has resulted in its frequent detection in environmental surveys. CYP2C19 and CYP3A4 are crucial for converting clothianidin to desmethyl-clothianidin (dm-clothianidin). The expression of these CYP450s can be significantly influenced by genetic polymorphisms . The objective of our research was to examine the catalytic effects of 27 CYP3A4 variants and 31 CYP2C19 variants on the metabolism of clothianidin within recombinant insect microsomes . These variants were assessed through a well-established incubation procedure. In addition, the concentration of its metabolite dm-clothianidin was quantified by employing an ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Lastly, the kinetic parameters of these CYP3A4 and CYP2C19 variants were calculated by applying Michaelis-Menten kinetic analysis to fit the data. The observed changes in enzyme activity were related to the metabolic transformation of clothianidin to dm-clothianidin. In the CYP2C19 metabolic pathway, one variant (CYP2C19.23) showed no notable change in intrinsic clearance (CL int ), four variants (CYP2C19.29, .30, .31 and L16F) demonstrated a marked increase in CL int (110.86–183.46 %), and the remaining 25 variants exhibited a considerable decrease in CL int (26.38–89.79 %), with a maximum decrease of 73.62 % (CYP2C19.6). In the CYP3A4 metabolic pathway, 26 variants demonstrated significantly reduced CL int (10.54–52.52 %), with a maximum decrease of 89.46 % (CYP3A4.20). Our results suggested that most variants of CYP3A4 and CYP2C19 significantly altered the enzymatic activities associated with clothianidin metabolism to various degrees. This study provides new insights into assessing the metabolic behavior of pesticides and delivers crucial data that can guide clinical detoxification strategies.
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ACS Applied Nano Materials
·
2024
·
IF 5.3
Li Liu,Maomin Peng,Ke Xu,Hong Xia,Xitian Peng,Lijun Peng,Youxiang Zhou,Jin Z. Zhang
Highly stable and photoluminescent methylammonium lead halide perovskite quantum dots (MAPbBr3 PQDs) have been synthesized and applied for the fluorescence quenching detection of clothianidin in fruit and vegetable samples. Characterizations using different techniques, including photoluminescence (PL), X-ray diffraction (XRD), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), and time-resolved PL (TRPL), show that static quenching is the dominant fluorescence quenching mechanism of MAPbBr3 PQDs with clothianidin. Hydrogen bonds or van der Waals forces play major roles in the MAPbBr3 PQDs–clothianidin interaction. Under optimized conditions, the relative PL intensity of MAPbBr3 PQDs is linear to the concentration of clothianidin in the 0.0 and 20.0 mg/L range with a detection limit of 0.17 μg/kg. Excellent recoveries of 79.5–115.4% were acquired for radish and banana samples with a relative standard deviation below 9.4%. These results indicate that PQDs can be used for qualitative and quantitative identification of clothianidin, providing an effective detection method for risk prevention and control of neonicotinoid pesticide residues in agricultural products.
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FOOD CHEMISTRY
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2015
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IF 3.391
Enze Sheng,Haiyan Shi,Liangliang Zhou,Xiude Hua,Lu Feng,Tong Yu,Minghua Wang
Europium (Eu 3+ ) and samarium (Sm 3+ ) were used as fluorescent labels to develop a highly sensitive dual-labeled time-resolved fluoroimmunoassay (TRFIA) for detect clothianidin and diniconazole in food samples. Under the optimized assay conditions, 50% inhibition concentration (IC 50 ) and the limit of detection (LOD, IC 10 ) of clothianidin were 5.08 and 0.021 μg/L, and 13.14 and 0.029 μg/L for diniconazole. The cross-reactivities (CRs) were negligible except dinotefuran (9.4%) and uniconazole (4.28%). The recoveries of clothianidin and diniconazole ranged from 79.3% to 108.7% in food samples. The results of TRFIA for the authentic samples were validated by gas chromatography (GC) analyses, and a satisfactory correlations were obtained. These results indicated that the method was an alternative tool for simultaneous detection of clothianidin and diniconazole in food samples.
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INDUSTRIAL CROPS AND PRODUCTS
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2026
·
IF 6.4
Jiantao Fu,Lijun Chen,Xiankun Shang,Jihu Li,Qinghua Zen,Jinjian Wu,Yanping Luo,Yuxing An
SA@BC@CTD hydrogel microspheres were successfully prepared. • High drug loading (16.15%) and encapsulation efficiency (91.68%) were achieved. • The formulation exhibited environmentally responsive controlled release. • Long-term pest control effectiveness was maintained for up to 60 days. • Reduced and delayed acute toxicity to earthworms compared with conventional CTD was observed. Sugarcane ( Saccharum officinarum L.) constitutes a major sugar crop and renewable bioenergy feedstock that plays a vital role in shaping the global agricultural economy and energy systems. However, sugarcane production is significantly threatened by pest infestations, which reduce yield and long-term sustainability levels. Although chemical pesticides are widely employed for pest control, their excessive use has caused significant environmental concerns and potential risks to human and ecological health. To address these challenges, nanobiochar (BC)-based clothianidin (CTD) composite hydrogel microspheres (SA@BC@CTD) were developed via the use of sodium alginate (SA) as the matrix and BC as a functional carrier via Ca²⁺ crosslinking. The release behavior, insecticidal performance, crop safety, and environmental risks of the prepared microspheres were comprehensively characterized through laboratory, pot, and field experiments. Compared with SA@CTD, SA@BC@CTD exhibited a stable three-dimensional porous structure, with a drug loading content of 16.15 ± 0.53% and an encapsulation efficiency of 91.68 ± 1.72%, representing increases of 87.40% and 16.70%, respectively. CTD release was significantly affected by pH and ionic strength, with optimal controlled-release behavior under neutral conditions (pH of 7) and high ionic strength (1 mol/L NaCl), with the duration of sustained release exceeding 96 h. The results of indoor bioassays demonstrated that compared with SA@CTD, SA@BC@CTD exhibited significantly increased concentration-dependent insecticidal activity against Spodoptera frugiperda Smith (third-instar larvae) and Chilo sacchariphagus Bojer (second-instar larvae). The results of pot experiments demonstrated that the application of SA@BC@CTD significantly enhanced sugarcane growth (plant height: 134.05 ± 24.17 cm; fresh weight: 431.35 ± 46.63 g at 60 d) while maintaining high insecticidal effectiveness (mortality rates of 35.12 ± 3.26% for S. frugiperda and 37.64 ± 3.52% for C. sacchariphagus at 60 d). This effect could be attributed to the sustained release of CTD and its enhanced translocation to aerial tissues, as indicated by a leaf transfer coefficient of 0.33 ± 0.02 at 60 d. Field trials confirmed that the use of SA@BC@CTD provided long-term control of dead sugarcane heart (81.59 ± 4.22%) at 60 d, thereby significantly outperforming conventional CTD granules. Moreover, acute toxicity tests using Eisenia fetida Savigny showed reduced toxicity compared with conventional CTD. In conclusion, this study provides a feasible strategy for sustained-release pesticide formulations with prolonged efficacy, enabling reduced pesticide use and sustainable sugarcane cultivation. Download: Download high-res image (141KB) Download: Download full-size image
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Environmental Technology & Innovation
·
2026
·
IF 7.3
Saqib Farooq,Jia Xie,Jie Zhang,Jinhua Li,Weiguo Miao,Yongmei Fan
This study reports the development of magnetic molecularly imprinted polymers (MMIPs) synthesized by using dual-functional monomers for selective extraction of clothianidin (CLO). A novel dual-functional monomer system, combining a supramolecular deep eutectic solvent with methacrylic acid, was employed to improve the imprinting efficiency and molecular recognition. The MMIPs showed rapid adsorption kinetics, reaching equilibrium within 12 min with a maximum adsorption capacity of 57.77 mg g⁻¹. They also possessed a porous magnetic structure, enabling quick separation and excellent selectivity toward CLO. The optimized extraction conditions yielded recoveries of 91.46–100.20% and detection and quantification limits of 0.003 and 0.010 mg kg⁻¹, respectively, in spiked food and soil samples analyzed by HPLC. The polymers exhibited excellent selectivity and stability, retaining about 90% adsorption efficiency in seven reuse cycles. This method offers a sensitive, reliable, and reusable approach for CLO residue monitoring in complex matrices.
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