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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
线粒体呼吸链复合体Ⅰ又称 NADH-CoQ 还原酶或 NADH 脱氢酶，广泛存在于动物、植物、微生物和培养细胞的线粒体中，是线粒体内膜中最大的蛋白复合物。该酶催化一对电子从NADH 传递给CoQ，同时使O₂还原生成O₂•⁻，是线粒体呼吸电子传递链上产生O₂•⁻的主要步骤。该酶活性强弱不仅可以反映呼吸电子传递链状态，还可以反映活性氧的生成状态。 本试剂盒检测原理是线粒体呼吸链复合体Ⅰ能够催化 NADH 脱氢生成NAD⁺，测定NADH在340nm处吸光度值的变化即可计算样本中线粒体呼吸链复合体Ⅰ的活性。
Mitochondrial respiratory chain complex I, also known as NADH-CoQ reductase or NADH dehydrogenase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is the largest protein complex in the inner mitochondrial membrane. This enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, while simultaneously reducing O₂ to generate O₂•⁻ (superoxide anion). It represents the primary step in the mitochondrial respiratory electron transport chain responsible for O₂•⁻ production. The activity level of this enzyme not only reflects the status of the respiratory electron transport chain but also indicates the state of reactive oxygen species generation. The detection principle of this kit is based on the ability of mitochondrial respiratory chain complex I to catalyze the dehydrogenation of NADH, generating NAD⁺. By measuring the change in absorbance of NADH at 340 nm, the activity of mitochondrial respiratory chain complex I in the sample can be calculated.
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