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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
线粒体呼吸链复合体Ⅱ又称琥珀酸-辅酶 Q 还原酶，广泛存在于动物、植物、微生物和培养细胞的线粒体中，催化琥珀酸氧化生成延胡索酸，同时辅基 FAD 还原为 FADH₂，后者进一步还原氧化型辅酶Q生成还原型辅酶 Q，这是呼吸电子传递链的支路。 本试剂盒检测原理是线粒体呼吸链复合体Ⅱ的催化产物还原型辅酶 Q 可进一步还原2,6-二氯吲哚酚，2,6-二氯吲哚酚在 605 nm 有特征吸收峰，通过检测 2,6-二氯吲哚酚的减少速率来计算该酶活性。
Mitochondrial respiratory chain complex II, also known as succinate-coenzyme Q reductase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It catalyzes the oxidation of succinate to fumarate, while the prosthetic group FAD is reduced to FADH₂. The latter further reduces oxidized coenzyme Q to generate reduced coenzyme Q, representing a branch pathway of the respiratory electron transport chain. The detection principle of this kit is based on the fact that the catalytic product of mitochondrial respiratory chain complex II, reduced coenzyme Q, can subsequently reduce 2,6-dichlorophenolindophenol (DCIP). DCIP exhibits a characteristic absorption peak at 605 nm. By monitoring the rate of decrease in DCIP absorbance, the activity of this enzyme can be calculated.
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