线粒体呼吸链复合体Ⅲ（EC 1.10.2.2）又称 CoQ-细胞色素 C 还原酶，广泛存在于动物、植物、微生物和培养细胞的线粒体中，是线粒体呼吸电子传递链主路和支路的共有成分，负责把还原型CoQ的氢传递给细胞色素 C，生成还原型细胞色素 C。线粒体呼吸链复合体Ⅲ是一个多亚基的膜蛋白质复合体，它是参与光合作用和呼吸电子传递链的一个基本组分。 本试剂盒检测原理是线粒体呼吸链复合体Ⅲ把还原型 CoQ 的氢传递给细胞色素C，生成还原型细胞色素C，与氧化型细胞色素 C 不同，还原型细胞色素 C 在 550 nm 有特征光吸收，因此550 nm光吸收增加速率能够反映线粒体呼吸链复合体Ⅲ活性，通过检测 550 nm 处吸光度的变化即可计算出样本中线粒体呼吸链复合体Ⅲ活性。
Mitochondrial respiratory chain complex III (EC 1.10.2.2), also known as coenzyme Q-cytochrome c reductase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is a common component of both the main and branch pathways of the mitochondrial respiratory electron transport chain, responsible for transferring hydrogen from reduced coenzyme Q to cytochrome c, thereby generating reduced cytochrome c. Mitochondrial respiratory chain complex III is a multisubunit membrane protein complex and serves as a fundamental component involved in both photosynthesis and the respiratory electron transport chain. The detection principle of this kit is based on the activity of mitochondrial respiratory chain complex III, which transfers hydrogen from reduced coenzyme Q to cytochrome c, producing reduced cytochrome c. Unlike oxidized cytochrome c, reduced cytochrome c exhibits characteristic light absorption at 550 nm. Therefore, the rate of increase in light absorption at 550 nm reflects the activity of mitochondrial respiratory chain complex III. By measuring the change in absorbance at 550 nm, the activity of mitochondrial respiratory chain complex III in the sample can be calculated.
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