BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
线粒体呼吸链复合体Ⅴ，又称F₁F₀-ATP合酶，由F₁和F₀两个亚单位组成，是线粒体氧化磷酸化和叶绿体光合磷酸化合成 ATP 的关键酶，广泛存在于动物、植物、微生物和培养细胞的线粒体中。该酶利用呼吸链产生的质子电化学梯度催化 ATP 合成，也可通过逆反应过程水解 ATP。 本试剂盒检测原理是线粒体呼吸链复合体Ⅴ水解 ATP 产生 ADP 和 Pi，Pi与钼酸铵还原剂的作用下生成深蓝色的钼蓝，测定其在 660 nm 处吸光度的变化即可计算样本中线粒体呼吸链复合体Ⅴ的活性。
Mitochondrial respiratory chain complex V, also known as F₁F₀-ATP synthase, consists of two subunits, F₁ and F₀. It is a key enzyme for ATP synthesis in mitochondrial oxidative phosphorylation and chloroplast photophosphorylation, widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. This enzyme utilizes the proton electrochemical gradient generated by the respiratory chain to catalyze ATP synthesis and can also hydrolyze ATP through a reverse reaction. The detection principle of this kit is based on the hydrolysis of ATP by mitochondrial respiratory chain complex V, producing ADP and inorganic phosphate (Pi). In the presence of a reducing agent, Pi reacts with ammonium molybdate to form a dark blue phosphomolybdenum complex. By measuring the change in absorbance at 660 nm, the activity of mitochondrial respiratory chain complex V in the sample can be calculated.
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