支原体又称霉形体，归属于柔膜体纲，没有细胞壁，也不能合成细胞壁前体，有三层结构的单位膜，不能维持固定的形态而呈现多形性它广泛存在于自然界中，有80余种，污染细胞最常⻅的支原体包括发酵支原体、猪鼻支原体、口腔支原体、精氨酸支原体、梨支原体、唾液支原体和人型支原体。支原体通常附着在细胞的表面，并影响其宿主细胞的生理、遗传等多方面的正常功能，用这些污染后貌似正常的细胞做试验或生产，将会严重影响结果。
支原体清除剂是解决细胞培养中支原体污染严重问题的有效方法。本试剂是抗生素衍生物，通过抑制支原体DNA旋转酶解旋酶去除支原体感染。在推荐使用浓度情况下，能够有效去除多种支原体，并且具有预防支原体污染复发的作用。具有很强的抑制支原体活性的能力，一周之内即可从被污染的培养细胞中彻底去除支原体。
1. 具体很强的抑制支原体活性的能力，从污染的培养中彻底清除支原体：通过抑制DNA促旋酶清除支原体污染，该酶是微生物DNA复制过程中必不可少的酶类。
2. 活性范围广：活性在细胞培养物中可以维持⻓达7天，对多种支原体株系均有作用。
3. 作用浓度低，细胞毒性小：在推荐浓度下使用，细胞毒性极小，因此也可以作为预防支原体污染使用的试剂。实验表明，与其他品牌的同类产品相比，对正常培养的细胞没有致死效应。
4. 防止支原体污染复发：一旦使用了，培养细胞就不会再受到同样的支原体污染，从而起到预防污染复发的效果。
5. 使用方便：使用非常方便，即拆即用，只要添加到支原体污染的培养细胞中孵化一周即可。
操作说明：
1.将支原体去除试剂 (1000×) 加到被支原体污染的细胞培养基，终浓度为1×，孵化一个星期。（比如在一个25 cm²的培养瓶中，10mL的培养基添加10μL的支原体去除试剂 (1000×)）
2.使用含有相同浓度支原体去除试剂 (1000×) 的培养基换液。
3.使用不含支原体去除试剂 (1000×) 的细胞培养基多次换液以确认支原体的污染不再发生。
4.可以用一个支原体检测试剂盒来检测污染。
5.如果担心血清或胰蛋白酶中存在支原体，在培养基中添加终浓度为1×的支原体去除试剂，可以预防支原体污染。
注意事项：
1. 本产品仅用于研发，仅仅作为一种细胞培养基的支原体清除剂。
2. 使用该产品时要注意佩戴手套口罩，遵守实验室规范。
3. 使用推荐浓度时，对细胞毒性及低。但是，对于任何特定功能的细胞，建议保留理想的细胞特征以供处理后确认。
Mycoplasmas, also known as mycoplasmatales, belong to the class Mollicutes. They have no cell walls and cannot synthesize cell wall precursors. They possess a three-layered unit membrane and exhibit pleomorphism without maintaining a fixed shape. Widespread in nature, there are more than 80 species of mycoplasmas. The most common mycoplasmas that contaminate cell cultures include Mycoplasma fermentans, Mycoplasma hyorhinis, Mycoplasma orale, Mycoplasma arginini, Mycoplasma pirum, Mycoplasma salivarium and Mycoplasma hominis. Mycoplasmas usually attach to the surface of host cells and disrupt their normal physiological, genetic and other functions. Experiments and production using these seemingly normal but contaminated cells will lead to severely compromised results.
Mycoplasma eradicator provides an effective solution to severe mycoplasma contamination in cell culture. This reagent is an antibiotic derivative that eliminates mycoplasma infection by inhibiting mycoplasma DNA gyrase (helicase). At the recommended working concentration, it effectively eliminates multiple mycoplasma species and prevents recurrent mycoplasma contamination. It exhibits potent anti-mycoplasma activity and can completely eradicate mycoplasma from contaminated cultured cells within one week.
1. Potent anti-mycoplasma activity for complete mycoplasma elimination from contaminated cultures: It removes mycoplasma contamination by inhibiting DNA gyrase, an essential enzyme for microbial DNA replication.
2. Broad-spectrum activity: Its activity lasts up to 7 days in cell cultures and works against various mycoplasma strains.
3. Low effective concentration and minimal cytotoxicity: When used at the recommended concentration, it shows very low cytotoxicity and can also be used for preventive mycoplasma control. Experiments confirm it causes no lethal effects on normally cultured cells compared with similar products from other brands.
4. Prevention of recurrent mycoplasma contamination: After treatment, cultured cells are protected against re-infection by the same mycoplasma strains, preventing recurring contamination.
5. Easy to use: Ready-to-use formulation. Simply add it to mycoplasma-contaminated cell cultures and incubate for one week.
Instructions for Use:
1. Add the Mycoplasma Removal Agent (1000×) to the medium of mycoplasma-contaminated cells to achieve a final concentration of 1×, and incubate for one week. (Example: Add 10 μL of Mycoplasma Removal Agent (1000×) to 10 mL medium in a 25 cm² cell culture flask.)
2. Perform medium changes using fresh medium supplemented with the same 1× concentration of Mycoplasma Removal Agent (1000×).
3. Conduct repeated medium changes with mycoplasma-free medium (without the Mycoplasma Removal Agent (1000×)) to confirm no further mycoplasma contamination.
4. Verify the absence of mycoplasma using a mycoplasma detection kit.
5. To prevent contamination originating from serum or trypsin, add the 1000× Mycoplasma Eradicator to the medium at a final concentration of 1× as a precaution.
Precautions:
1. For research use only. Intended solely as a mycoplasma removal reagent for cell culture media.
2. Wear gloves and a mask during handling, and follow standard laboratory safety protocols.
3. Cytotoxicity is very low at the recommended concentration. For specialized cell lines, it is advised to verify that key cellular characteristics remain intact after treatment.
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