计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,充氩,干燥。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
MitoTam bromide, hydrobromide, a Tamoxifen derivative, is an electron transport chain ( ETC ) inhibitor. MitoTam bromide, hydrobromide reduces mitochondrial membrane potential in senescent cells and affects mitochondrial morphology. MitoTam bromide, hydrobromide is an effective anticancer agent, suppresses respiratory complexes (CI-respiration) and disrupts respiratory supercomplexes (SCs) formation in breast cancer cells.
In Vitro
MitoTam (0.5 μM-56 μM; 24 hours) kills breast cancer cell Lines and nonmalignant cells with an IC 50 range from 0.65 μM to 55.9 μM. MitoTam (2.5 μM; 2-24 hours) results in stronger activation of the apoptotic pathway in MCF7 Her2 high cells compared with mock MCF7 cells. MitoTam (0.05 μM-1 μM; 3 days) causes a concentration-dependent induction of apoptosis in breast cancer cells, while there was no effect for non-malignant breast epithelial cellsMCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: Breast Cancer Cell Lines: BT474, MCF7, MCF7 Her2 high , MCF7 Her2 low , MDA-MB-231, MDA-MB-436, MDA-MB-453, SK-BR-3, T47D; NeuTL cells; Nonmalignant Cells: A014578, H9c2 cells Concentration: 0.5 μM-56 μM Incubation Time: 24 hours Result: Killed breast cancer cells MCF7, MCF7 Her2 high , MCF7 Her2 low with IC 50 values of 1.25 μM, 0.65 μM and 1.45 μM respectively. Western Blot AnalysisCell Line: MCF7 mock cells, MCF7 Her2 high cells Concentration: 2.5 μM Incubation Time: 2 hours, 4 hours, 8 hours, 16 hours, 24 hours Result: Revealed accelerated cleavage of procaspase-9, Parp1/2 and proapoptotic Bax, decreased the antiapoptotic Bcl-2 protein in Her2high cells. Apoptosis AnalysisCell Line: MCF-7 cells, 4T1 cells and MCF-10a cells Concentration: 0.05 μM-1 μM Incubation Time: 3 days Result: Resulted in apoptosis in MCF7 and 4T1 cells.
In Vivo
MitoTam (intraperitoneal injection; 2 μg/g; once a week; 4 weeks) decreases β-gal staining of lungs from MitoTam-treated mice, accompaning by a inhibition in the expression of senescence markers p16Ink4a, p21waf1 and PAI comparing control mice sup>. MitoTam (intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks) inhibits growth of syngeneic tumors by 80% . MitoTam (intraperitoneal injection; 0.25 μmol/mouse; twice a week; 2 weeks) slows down the growth of MCF7 mock tumors and stops tumor progression after two doses; suppresses Her2 high carcinomas decreased threefold from the original size with complete disappearance . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: 18-month-old or 2-month-old FVB/N miceDosage: 2 μg/g Administration: Intraperitoneal injection; 2 μg/g; once a week; 4 weeks Result: Eliminated senescent cells also in vivo. Animal Model: FVB/N c-neu mouse Dosage: 0.54 μmol/mouse Administration: Intraperitoneal injection; 0.54 μmol/mouse; twice a week; 2 weeks Result: Suppressed Her2 high breast carcinomas. Animal Model: Balb/c nude mice with MCF7 mock or MCF7 Her2 high cells Dosage: 0.25 μmol/mouse/dose Administration: Intraperitoneal injection; 0.25 μmol/mouse/dose; twice a week; 2 weeks Result: Prevented reaching the ethical endpoint in all situations, slowed down the growth of MCF7 mock tumors and suppressed Her2 high carcinomas decreased.
Form:Solid
规格
别名
MitoTam 溴化物、氢溴化物
规格或纯度
≥98%
英文名称
MitoTam bromide, hydrobromide
生化机理
MitoTam溴氢溴酸盐是一种他莫昔芬衍生物，是一种电子传递链（ETC）抑制剂。MitoTam 溴氢溴酸盐可降低衰老细胞的线粒体膜电位并影响线粒体形态。米托溴铵
储存条件
2-8°C储存,充氩,干燥
运输条件
低温运输
作用类型
抑制剂
纯度
≥98%
名称和识别符
分子类型
小分子
分子量
905.82
技术文档
高级数据
化学和物理性质
溶解性
DMSO : 240 mg/mL (264.95 mM; Need ultrasonic)
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥98%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于2–8 °C、氩气保护、干燥条件下保存。本品在氩气保护下供应，每次取用后请在惰性气体下重新密封。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 1634624-73-9，分子式为 C52H60Br2NOP，分子量为 905.82 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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