计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
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产品简介
试剂盒提供了一种简单、快速、高效的血片DNA提取方法，适用于从血片中提取 基因组DNA。在高盐存在时， DNA结合于硅基包被的Magbeads表面。漂洗后，高纯 度的DNA被洗脱于Buffer EB或去离子水中。纯化得到的DNA纯度好（A260/280的比值 在1.7-1.9之间），完整度高（>15 kb），可用于二代测序、定量PCR、芯片检测等下 游实验。
自备仪器、试剂
1）恒温混匀仪
2）2/15 ml磁力架
3）32通道核酸提取仪
4）96通道核酸提取仪
5）96 DW Plate
6）8 channel Comb
7）Spin tips pack
8）无水乙醇
实验前准备及重要注意事项
1.第一次使用前按照试剂瓶标签向Buffer CW1、Buffer GW1和Buffer GW2中加入无 水乙醇并做好标记。
2.Magbeads严禁冰冻、离心。冰冻、离心可能会对Magbeads造成不可逆的损害。
操作步骤
Ⅰ.手动单管操作
1.用打孔钳从血斑中取1片直径为6 mm 的血斑或4片直径为3 mm 的血斑（根据实际 情况）放入2.0 mL的离心管中。
2.向离心管中加入40 μL Proteinase K和300 μL Buffer WSL，之后将离心管放于 75℃、 1200 rpm的恒温混匀仪上震荡裂解45分钟，形成Lysate，将离心管从恒温 混合仪上取下，短暂离心，取上清。
注意：如无恒温混匀仪，将离心管涡旋震荡10秒钟后放于75℃水浴锅中孵育30分钟，期间每隔10分钟涡旋震荡10秒钟。
3.将上清液吸至一支新的2.0 mL离心管，加入300 μL Buffer MSL、300 μL 异丙醇和 20 μL Magbeads V3。之后将离心管放于25℃、 1600 rpm的恒温混匀仪上震荡裂 解15分钟或将离心管连续颠倒混匀15分钟。
4.将离心管放于磁力架上静置1分钟，待Magbeads完全吸附于离心管侧壁后充分弃 去溶液（保持离心管固定于磁力架上）。
5.将离心管从磁力架上取下，加入900 μL Buffer CW1（使用前请检查是否已加入无 水乙醇） 后涡旋点震1分钟或涡旋震荡5秒钟后放于25℃、 1600 rpm的恒温混匀仪 上震荡混匀2分钟（震荡过程中确Magbeads处于混匀状态）。之后将离心管放于 磁力架上静置1分钟，待Magbeads完全吸附于离心管侧壁后轻轻颠倒磁力架将离 心管盖上的杂质洗落后彻底弃去溶液（保持离心管固定于磁力架上）。
6.将离心管从磁力架上取下，加入500 μL Buffer GW1（使用前请检查是否已加入无 水乙醇） 后涡旋点震1分钟或涡旋震荡5秒钟后放于25℃、 1600 rpm的恒温混匀仪 上震荡混匀2分钟（震荡过程中确Magbeads处于混匀状态）。之后将离心管放于 磁力架上静置1分钟，待Magbeads完全吸附于离心管侧壁后轻轻颠倒磁力架将离 心管盖上的杂质洗落后彻底弃去溶液（保持离心管固定于磁力架上）。
7.将离心管从磁力架上取下，加入900 μL Buffer GW2（使用前请检查是否已加入无 水乙醇）后涡旋点震1分钟或涡旋震荡5秒钟后放于25℃、 1600 rpm的恒温混匀仪 上震荡混匀2分钟（震荡过程中确Magbeads处于混匀状态）。之后将离心管放于 磁力架上静置1分钟，待Magbeads完全吸附于离心管侧壁后轻轻颠倒磁力架将离 心管盖上的杂质洗落后彻底弃去溶液（保持离心管固定于磁力架上）。
8.将离心管从磁力架上取下，加入300 μL 75%乙醇后涡旋点震1分钟或涡旋震荡5秒 钟后放于25℃、 1600 rpm的恒温混匀仪上震荡混匀2分钟（震荡过程中确 Magbeads处于混匀状态）。之后将离心管放于磁力架上静置 1分钟，待 Magbeads完全吸附于离心管侧壁后轻轻颠倒磁力架将离心管盖上的杂质洗落后 彻底弃去溶液（保持离心管固定于磁力架上）。
9.保持离心管固定于磁力架上，用移液器进一步去除离心管管底和管盖上的溶液， 之后室温放置5-10分钟，使乙醇挥发干净。
10.将离心管从磁力架上取下，加入50-200 μL Buffer EB。涡旋震荡使磁珠完全悬浮 于洗脱液中后将其放于56℃、 1600 rpm的恒温混匀仪上震荡洗脱10分钟，或将离 心管放于56℃水浴锅中孵育10分钟，期间每隔3分钟涡旋震荡10秒钟。
11.将离心管放于磁力架上静置2分钟，待Magbeads完全吸附于离心管侧壁后用移液 器将洗脱液转移至新的离心管中-20℃保存备用。
Ⅱ.与CWE2100匹配
1.用打孔钳从血斑中取1片直径为6 mm 的血斑或4片直径为3 mm 的血斑（根据实际 情况）放入2.0 mL的离心管中。
2.向离心管中加入40 μL Proteinase K和300 μL Buffer WSL，之后将离心管放于 75℃、 1200 rpm的恒温混匀仪上震荡裂解45分钟，形成Lysate。
3.按下表向96DW深孔板中加入相应试剂。
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4.将加入试剂的深孔板和磁套放于CWE2100/CWE3200的相应位置，运行血片提取 程序，约40分钟后程序运行结束，取出深孔板和磁套。
5.将深孔板6&12列中的洗脱产物转移至1.5 mL离心管中低温保存。
Ⅲ.与CWE960匹配
1.用打孔钳从血斑中取1片直径为6 mm 的血斑或4片直径为3 mm 的血斑（根据实际 情况）放入2.0 mL的离心管中。
2.向离心管中加入40 μL Proteinase K和300 μL Buffer WSL，之后将离心管放于 75℃、 1200 rpm的恒温混匀仪上震荡裂解45分钟，形成Lysate。
3.按下表向96DW深孔板中加入相应试剂
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4.将加入试剂的深孔板和磁套放于CWE960的相应位置，运行血片提取程序，约40 分钟后程序运行结束，取出深孔板和磁套。
5.将Plate 6中的洗脱产物转移至1.5 mL离心管中低温保存。
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Product Introduction：
The reagent kit provides a simple, fast, and efficient method for extracting genomic DNA from blood samples. In the presence of high salt, DNA binds to the surface of silica coated Magheads. After rinsing, high-purity DNA is eluted in Buffer EB or deionized water. The purified DNA has good purity (A260/280 ratio between 1.7-1.9) and high integrity (>15 kb), and can be used for downstream experiments such as second-generation sequencing, quantitative PCR, and chip detection.
Self provided instruments and reagents
1) Constant temperature mixer
2) 2/15 ml magnetic frame
3) 32 channel nucleic acid extractor
4) 96 channel nucleic acid extractor
5) 96 DW Plate
6) 8 channel Comb
7) Spin tips pack
8) Anhydrous ethanol
Preparation and important precautions before the experiment
1.Before the first use, add anhydrous ethanol to Buffer CW1, Buffer GW1, and Buffer GW2 according to the label of the reagent bottle and mark them properly.
2.Magheads are strictly prohibited from freezing or centrifugation. Freezing and centrifugation may cause irreversible damage to Magheads.
Operation steps
I. Manual single tube operation
1. Use punching forceps to take 1 blood spot with a diameter of 6 mm or 4 blood spots with a diameter of 3 mm (depending on the actual situation) from the blood spot and place them in a 2.0 mL centrifuge tube.
2. Add 40 to the centrifuge tube μ L Protein K and 300 μ L Buffer WSL, then place the centrifuge tube on a constant temperature mixer at 75 ℃ and 1200 rpm, shake and crack for 45 minutes to form Lysate. Remove the centrifuge tube from the constant temperature mixer, centrifuge briefly, and take the supernatant.
Attention: If there is no constant temperature mixer, vortex the centrifuge tube for 10 seconds and incubate it in a 75 ℃ water bath for 30 minutes. During this period, vortex every 10 minutes for 10 seconds.
3. Suck the supernatant into a new 2.0 mL centrifuge tube and add 300 μ L Buffer MSL, 300 μ L isopropanol and 20 μ L Magheads V3. Afterwards, place the centrifuge tube on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and crack for 15 minutes, or invert the centrifuge tube and mix continuously for 15 minutes.
4. Place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, discard the solution thoroughly (keep the centrifuge tube fixed on the magnetic stand).
5. Remove the centrifuge tube from the magnetic frame and add 900 μ L Buffer CW1 (please check if anhydrous ethanol has been added before use), vortex point shake for 1 minute or vortex shake for 5 seconds, and then place it on a constant temperature mixer at 25 ℃ and 1600 rpm to shake and mix for 2 minutes (ensure that Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
6. Remove the centrifuge tube from the magnetic frame and add 500 μ L Buffer GW1 (please check if anhydrous ethanol has been added before use), vortex point shake for 1 minute or vortex shake for 5 seconds, and then place it on a constant temperature mixer at 25 ℃ and 1600 rpm to shake and mix for 2 minutes (ensure that Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
7. Remove the centrifuge tube from the magnetic frame and add 900 μ L Buffer GW2 (please check if anhydrous ethanol has been added before use), vortex point shake for 1 minute or vortex shake for 5 seconds, then place it on a constant temperature mixer at 25 ℃ and 1600 rpm, shake and mix for 2 minutes (ensure that Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
8. Remove the centrifuge tube from the magnetic frame and add 300 μ After shaking with 75% ethanol for 1 minute or 5 seconds, place the mixture on a constant temperature mixer at 25 ℃ and 1600 rpm for 2 minutes (ensure that the Magheads are in a mixed state during the shaking process). Afterwards, place the centrifuge tube on a magnetic stand and let it stand for 1 minute. After Magheads are completely adsorbed on the side wall of the centrifuge tube, gently invert the magnetic stand and wash the impurities on the centrifuge tube cover to completely discard the solution (keep the centrifuge tube fixed on the magnetic stand).
9. Keep the centrifuge tube fixed on the magnetic frame, use a pipette to further remove the solution from the bottom and cover of the centrifuge tube, and then leave it at room temperature for 5-10 minutes to allow the ethanol to evaporate completely.
10. Remove the centrifuge tube from the magnetic frame and add 50-200 μ L Buffer EB. Vortex oscillation causes the magnetic beads to completely suspend in the eluent and then place them on a constant temperature mixer at 56 ℃ and 1600 rpm for 10 minutes of shaking and elution, or incubate the centrifuge tube in a 56 ℃ water bath for 10 minutes, with vortex oscillation every 3 minutes for 10 seconds.
11. Place the centrifuge tube on a magnetic stand and let it stand for 2 minutes. After Magheads are completely adsorbed on the side wall of the centrifuge tube, transfer the eluent to a new centrifuge tube using a pipette and store at -20 ℃ for later use.
II. Matching with CWE2100
1. Use punching forceps to take 1 blood spot with a diameter of 6 mm or 4 blood spots with a diameter of 3 mm (depending on the actual situation) from the blood spot and place them in a 2.0 mL centrifuge tube.
2. Add 40 to the centrifuge tube μ L Protein K and 300 μ L Buffer WSL, then place the centrifuge tube on a constant temperature mixer at 75 ℃ and 1200 rpm, shake and crack for 45 minutes to form Lysate.
3. Add the corresponding reagents to the 96DW deep well plate according to the table below.
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4.Place the deep well plate and magnetic sleeve that have been added to the reagent at the corresponding positions of CWE2100/CWE3200, run the blood slide extraction program, and after about 40 minutes, the program ends. Remove the deep well plate and magnetic sleeve.
5.Transfer the elution products from columns 6 and 12 of the deep well plate to a 1.5 mL centrifuge tube for low-temperature storage.
III. Matching with CWE960
1. Use punching forceps to take 1 blood spot with a diameter of 6 mm or 4 blood spots with a diameter of 3 mm (depending on the actual situation) from the blood spot and place them in a 2.0 mL centrifuge tube.
2. Add 40 to the centrifuge tube μ L Protein K and 300 μ L Buffer WSL, then place the centrifuge tube on a constant temperature mixer at 75 ℃ and 1200 rpm, shake and crack for 45 minutes to form Lysate.
3. Add the corresponding reagents to the 96DW deep well plate according to the table below
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4. Place the deep well plate and magnetic sleeve that have been added to the reagent at the corresponding positions on CWE960, run the blood slide extraction program, and after about 40 minutes, the program ends. Remove the deep well plate and magnetic sleeve.
5. Transfer the elution products from Plate 6 to a 1.5 mL centrifuge tube for low-temperature storage.
规格
英文名称
Magbead Blood Spots DNA Kit
储存与运输
储存条件
室温
运输条件
常规运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
常见问题
本产品应如何储存？
请于室温条件下保存。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
试剂盒包含哪些组分？
完整组分列表见PDP和试剂盒说明书。个别组分的替换品可单独订购——请联系您的客户代表。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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