计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
产品内容
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产品简介
该试剂盒提供了一种简单、快速、高效的从新鲜组织中提取DNA的方法。组织裂 解后， DNA结合于硅基包被磁珠表面。漂洗后，高纯度的DNA洗脱于EB或去离子水 中。经过纯化的DNA可以直接用于PCR、 Real-time PCR、 SNP基因分型、 STR基因 分型、二代测序和药物基因组学研究等。
自备仪器、试剂
1. 全自动核酸提取仪
2. 无水乙醇、异丙醇
3. 96 DW Plate、 8 channel Comb
实验前准备及重要注意事项
1. 第一次使用前应按试剂瓶标签说明先在Buffer GW1和Buffer GW2中加入无水乙
醇。
2. 使用前请检查Buffer GTL和 Buffer GL是否出现结晶或者沉淀，如有结晶或者沉 淀，请将Buffer GTL和Buffer GL于56℃水浴重新溶解。
3. 如果下游实验对RNA污染比较敏感，可以在加入Buffer GL前加入2 μL DNase-Free 的RNase A（100 mg/mL ），试剂盒中的RNase A如果长时间不用，建议-20 ℃保 存。
4. 实验开始前将水浴锅或恒温混匀仪预热至56℃。
5. Magbeads PN严禁冰冻和高速离心，否则可能会对Magbeads PN造成不可逆的损 害。 Magbeads PN每次使用时请充分振荡混合均匀。
操作步骤（手动）
1. 称取20-30 mg组织，将组织在液氮中磨碎或使用组织匀浆机打碎，将打碎的组织 转移到1.5 mL 离心管中。
2. 向上述管中加入180 μL Buffer GTL和20 μL Proteinase K之后将离心管固定于 56℃、 1200 rpm恒温混匀仪上震荡裂解1小时或56℃水浴过夜至固体组织充分裂解， 即为Lysate。
3. 可选步骤，如果少量RNA的存在会对下游实验造成影响，建议向Lysate中加入2μL 浓度为100 mg/mL 的RNase A溶液，震荡混匀，室温放置2 min。
4. 向Lysate中加入200 μL Buffer GL，涡旋振荡混匀。
5. 向离心管中加入200 μL 异丙醇与10 μL Magbeads PN后涡旋震荡混匀5秒钟，之 后将离心管固定于25℃、 1600 rpm的恒温混匀仪上震荡结合10分钟。
6. 将离心管固定于磁力架上静置1分钟，之后弃去溶液。
7. 向离心管中加入750 μL Buffer GW1（使用前请检查是否已加入无水乙醇），涡旋 震荡5秒钟后放于25℃、 1600 rpm的恒温混匀仪上震荡结合2分钟。
8. 将离心管固定于磁力架上静置1分钟，之后弃去溶液。
9. 重复步骤7-8。
10. 向离心管中加入750 μL Buffer GW2（使用前请检查是否已加入无水乙醇），涡 旋震荡5秒钟后放于25℃、 1600 rpm的恒温混匀仪上震荡结合2分钟。
11. 将离心管固定于磁力架上静置1分钟，之后弃去溶液。
12. 重复步骤10-11。
13. 离心管短暂离心后，将其重新固定于磁力架上用移液器去除管底溶液，之后开盖 室温放置5-10分钟使乙醇充分挥发。
14. 向离心管中加入50-200 μL Buffer EB后涡旋震荡使磁珠充分悬浮于洗脱液中，之 后将离心管固定于56℃、 1600 rpm的恒温混匀仪上震荡洗脱10分钟。
15. 将离心管固定于磁力架上静置2分钟，之后将洗脱液转移至新的离心管中-20℃保 存备用。
操作步骤（与全自动核酸提取仪匹配）
1. 称取20-30mg组织，将组织在液氮中磨碎或使用组织匀浆机打碎，将打碎的组织 转移到1.5 mL 离心管中。
2. 向上述管中加入180 μL Buffer GTL和20 μL Proteinase K之后将离心管固定于 56℃、1200 rpm恒温混匀仪上震荡裂解1小时或56℃水浴过夜至固体组织充分裂 解，即为Lysate。
3. 可选步骤，如果少量RNA的存在会对下游实验造成影响，建议向Lysate中加入2μL 浓度为100 mg/mL 的RNase A溶液，震荡混匀，室温放置2 min。
4. 向Lysate中加入200 μL Buffer GL，涡旋振荡20 s，此刻离心管中液体即为Mixture。
5. 按下表向96 DW深孔板中加入试剂：
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注意：
在1&7列中，可先将异丙醇与Magbeads PN按比例混匀后再加入。
6. 将磁套与96 DW深孔板放入CWE2100中，运行“FFPE/Tissue DNA程序”。
7. 约32分钟后程序运行结束，将96DW深孔板和磁套从仪器中取出，把“6&12 Colume”中的洗脱产物转移至离心管中-20℃保存备用。
Product content
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Product Introduction
This kit provides a simple, fast, and efficient method for extracting DNA from fresh tissues. After tissue lysis, DNA binds to the surface of silicon coated magnetic beads. After rinsing, high-purity DNA is eluted in EB or deionized water. Purified DNA can be directly used for PCR, Real time PCR, SNP genotyping, STR genotyping, second-generation sequencing, and pharmacogenomic research.
Self provided instruments and reagents
1. Fully automatic nucleic acid extractor
2. Anhydrous ethanol and isopropanol
3. 96 DW Plate, 8 channel Comb
Preparation and important precautions before the experiment
Before the first use, anhydrous ethylene should be added to Buffer GW1 and Buffer GW2 according to the instructions on the reagent bottle label
Alcohol.
2. Before use, please check if there is any crystallization or precipitation in the Buffer GTL and Buffer GL. If there is any crystallization or precipitation, please dissolve them again in a 56 ℃ water bath.
3. If downstream experiments are sensitive to RNA contamination, 2 can be added before adding Buffer GL μ RNase A of L DNase Free (100 mg/mL). If RNase A in the reagent kit is not used for a long time, it is recommended to store it at -20 ℃.
4. Preheat the water bath or constant temperature mixer to 56 ℃ before the experiment begins.
5. Magheads PN is strictly prohibited from freezing and high-speed centrifugation, otherwise it may cause irreversible damage to Magheads PN. Please shake and mix Magheads PN thoroughly and evenly every time it is used.
Operation steps (manual)
1. Weigh 20-30 mg of tissue, grind it in liquid nitrogen or use a tissue homogenizer to crush it, and transfer the crushed tissue to a 1.5 mL centrifuge tube.
2. Add 180 to the above tube μ L Buffer GTL and 20 μ After L Protein K, fix the centrifuge tube on a constant temperature mixer at 56 ℃ and 1200 rpm, shake and lyse for 1 hour, or overnight in a water bath at 56 ℃ until the solid tissue is fully lysed, which is Lysate.
3. Optional steps, if the presence of a small amount of RNA will affect downstream experiments, it is recommended to add 2 to Lysate μ L RNase A solution with a concentration of 100 mg/mL, shake well and let stand at room temperature for 2 minutes.
4. Add 200 to Lysate μ L Buffer GL, vortex oscillation mixing.
5. Add 200 to the centrifuge tube μ L isopropanol and 10 μ After mixing the L Magheads PN vortex for 5 seconds, fix the centrifuge tube on a constant temperature mixer at 25 ℃ and 1600 rpm, and shake for 10 minutes.
6. Fix the centrifuge tube on a magnetic rack and let it stand for 1 minute, then discard the solution.
7. Add 750 to the centrifuge tube μ L Buffer GW1 (please check if anhydrous ethanol has been added before use), vortex for 5 seconds, and then place it on a constant temperature mixer at 25 ℃ and 1600 rpm for 2 minutes.
8. Fix the centrifuge tube on a magnetic rack and let it stand for 1 minute, then discard the solution.
9. Repeat steps 7-8.
10. Add 750 to the centrifuge tube μ L Buffer GW2 (please check if anhydrous ethanol has been added before use), vortex for 5 seconds, and then place it on a constant temperature mixer at 25 ℃ and 1600 rpm for 2 minutes.
11. Fix the centrifuge tube on a magnetic rack and let it stand for 1 minute, then discard the solution.
12. Repeat steps 10-11.
13. After a brief centrifugation of the centrifuge tube, fix it back on the magnetic frame and use a pipette to remove the solution at the bottom of the tube. Then, open the lid and let it sit at room temperature for 5-10 minutes to allow the ethanol to fully evaporate.
14. Add 50-200 to the centrifuge tube μ After vortex oscillation of L Buffer EB, the magnetic beads were fully suspended in the eluent. Then, the centrifuge tube was fixed on a constant temperature mixer at 56 ℃ and 1600 rpm, and shaken and eluted for 10 minutes.
15. Fix the centrifuge tube on the magnetic frame and let it stand for 2 minutes. Then transfer the eluent to a new centrifuge tube and store it at -20 ℃ for later use.
Operation steps (matching with fully automatic nucleic acid extractor)
1. Weigh 20-30mg of tissue, grind it in liquid nitrogen or use a tissue homogenizer to crush it, and transfer the crushed tissue to a 1.5 mL centrifuge tube.
2. Add 180 to the above tube μ L Buffer GTL and 20 μ After L Protein K, fix the centrifuge tube on a constant temperature mixer at 56 ℃ and 1200 rpm, shake and lyse for 1 hour, or overnight in a water bath at 56 ℃ until the solid tissue is fully lysed, which is Lysate.
3. Optional steps, if the presence of a small amount of RNA will affect downstream experiments, it is recommended to add 2 to Lysate μ L RNase A solution with a concentration of 100 mg/mL, shake well and let stand at room temperature for 2 minutes.
4. Add 200 to Lysate μ L Buffer GL, vortex oscillation for 20 seconds, at this moment, the liquid in the centrifuge tube is the Mixture.
5. Add reagents to the 96 DW deep well plate according to the following table:
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Attention:
In columns 1&7, isopropanol can be mixed with Magheads PN in proportion before being added.
6. Place the magnetic sleeve and 96 DW deep well plate into CWE2100 and run the "FFPE/Issue DNA program".
7. After about 32 minutes, the program runs and the 96DW deep well plate and magnetic sleeve are removed from the instrument. The elution products from "6&12 Columns" are transferred to a centrifuge tube and stored at -20 ℃ for later use.
规格
英文名称
Magbead Tissue DNA Kit
储存与运输
储存条件
室温
运输条件
常规运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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