基因编辑

超快速T4 DNA连接酶

规格或纯度 :
• 无动物源
• 无载体
• EnzymoPure™
• 无RNA酶
• 无菌
• 1000 U/μL

种属: 肠杆菌噬菌体T4(Enterobacteria phage T4)
Accession #: P00970
表达系统: E. coli
生物活性: One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA in 30min at 16℃ in 20µl of the assay mixture containing 50mM Tris, pH7.5, 10mM MgCl2, 10mM DTT, 1mM ATP, 25µg/ml BSA and a 5'-DNA termini concentration of 0.12µM (300µg/ml).200U equals 1 Weiss unit， Based on Weiss unit, the packaging of this product is 200U and 1000U respectively.