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基因编辑
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牛痘DNA拓扑异构酶I
- 种属: 痘苗病毒(Vaccinia virus)
- Accession #: P68698.1
- 表达系统: E. coli
- 生物活性: One unit of DNA Topoisomerase I from Vaccinia converts 1 µg of supercoiled closed circular pUC18 DNA to relaxed closed circular form in 30 min at 37°C.
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热敏磷酸酶
- 种属: 南极细菌TAB5(Antarctic bacterium TAB5)
- Accession #: 2W5W_B,Q9KWY4
- 表达系统: E. coli
- 生物活性: 1 unit refers to the 5 'concave end produced by restriction endonuclease digestion at 37 ℃ for 30 minutes. The enzyme amount required for dephosphorylation of µ g pUC19 vector DNA. The dephosphorylation standard refers to the self linking reaction Can inhibit>95% of DNA re circularization and detect it by transforming E. coli cells.
