计算溶液所需的质量、体积或浓度。
BioReagent,无菌过滤 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿拉丁Tris-HCl 抗体纯化中和缓冲浓缩液 (10×)是一种高浓度、即用稀释型溶液,专为抗体纯化流程中的快速pH调节而设计。这款10×浓缩液为需要大量中和缓冲液的实验室提供了一种经济且节省空间的解决方案,确保在低pH洗脱后能为纯化抗体提供最佳保护,防止酸诱导变性。
主要特点
10×浓缩配方:经济高效的解决方案,减少了储存空间和运输成本
易于1:10稀释:使用纯化水即可简单稀释至1×工作浓度
高缓冲容量:能有效中和包括pH 2.7-2.8洗脱缓冲液在内的酸性洗脱液
即用稀释便利性:预调pH值确保稀释后性能一致
抗体保护:防止因长时间酸暴露而影响抗体活性和稳定性
应用领域
蛋白质A/G/L亲和纯化后抗体的立即中和
兼容手动和自动化纯化系统
适用于中和来自各种亲和层析方法的洗脱液
理想用于研究规模和工艺开发应用
使用说明
用纯化水将10×浓缩液按1:10比例稀释,以获得1×工作液。
将1×工作液加入收集管中(推荐体积:预期洗脱液体积的1/10)。
将酸性洗脱液直接收集到中和缓冲液中。
轻轻混合,如有必要,请验证最终pH值。
注意事项
稀释后的工作液在4°C条件下可稳定保存4周。
为获得最佳效果,请使用预冷的纯化水进行稀释。
根据具体洗脱缓冲液的酸度调整中和液体积。
务必在收集洗脱液后立即进行中和。
Aladdin Neutralization Buffer (10× Tris-HCl, pH 9.0) is a high-concentration, ready-to-dilute solution specifically designed for rapid pH adjustment in antibody purification workflows. This 10X concentrate provides an economical and space-efficient solution for laboratories requiring large volumes of neutralization buffer, ensuring optimal protection of purified antibodies from acid-induced denaturation following low-pH elution
Applications
Key Features
10× Concentrated Formula: Cost-effective solution that reduces storage space and shipping costs
Easy 1:10 Dilution: Simple preparation to 1× working concentration with purified water
High Buffering Capacity: Effectively neutralizes acidic eluents including pH 2.7-2.8 elution buffers
Ready-to-Dilute Convenience: Pre-adjusted pH ensures consistent performance after dilution
Antibody Protection: Prevents prolonged acid exposure that can compromise antibody activity and stability
Applications
Immediate neutralization of antibodies following Protein A/G/L affinity purification
Compatible with both manual and automated purification systems
Suitable for neutralizing eluents from various affinity chromatography methods
Ideal for research-scale and process development applications
Instructions for Use
Dilute the 10× concentrate 1:10 with purified water to obtain 1× working solution
Add the 1× working solution to collection tubes (recommended: 1/10 of expected eluent volume)
Collect acidic eluate directly into the neutralization buffer
Mix gently and verify final pH if necessary
Notes
Diluted working solution is stable for 4 weeks when stored at 4°C
For optimal results, use pre-chilled purified water for dilution
Adjust neutralization volume according to specific elution buffer acidity
Always neutralize immediately after elution collection
| pH | pH 9.0 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N1456177 | |
| 分析证书 | N1456177 |