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BioReagent,无菌过滤 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
阿拉丁 Tris-HCl 抗体纯化中和缓冲液 (1×) 是一种即用型、高浓度的缓冲液,专门用于在抗体纯化过程中立即中和收集到的低pH洗脱液。该缓冲液能够快速调节pH值,以保护敏感的抗体免受酸诱导变性,确保生物活性得到最佳回收。
主要特点
即用型:预先调节至pH 9.0,无需稀释或进一步配制。
快速中和:在酸性洗脱后迅速恢复生理pH条件。
高缓冲容量:1M浓度确保能有效中和低pH洗脱缓冲液。
保护抗体完整性:防止长时间暴露于酸性环境导致抗体变性和活性丧失。
广泛兼容性:与所有酸性洗脱缓冲液(包括阿拉丁的IgG洗脱缓冲液,pH 2.7)兼容。
应用领域
蛋白质A/G/L亲和纯化后抗体的立即中和
兼容手动和自动化纯化系统
适用于中和来自各种亲和层析方法的洗脱液
理想用于研究规模和工艺开发应用
使用说明
在洗脱前,将中和缓冲液加入收集管中(推荐体积:预期洗脱液体积的1/10)。
将酸性洗脱液(例如,来自阿拉丁IgG洗脱缓冲液,pH 2.7)直接收集到该缓冲液中。
轻轻混合以确保完全中和。
如有必要,验证最终pH值;混合后应达到约中性pH(7.0-7.4)。
注意事项
为获得最佳抗体回收率,请在收集后立即进行中和。
对于更大规模的纯化,请按比例调整中和缓冲液体积。
可根据要求提供定制pH配方(pH 8.0-9.0)
Aladdin Neutralization Buffer (1M Tris-HCl, pH 9.0) is a ready-to-use, high-concentration buffer specifically formulated for the immediate neutralization of low-pH eluents collected during antibody purification processes. This buffer enables rapid pH adjustment to protect sensitive antibodies from acid-induced denaturation, ensuring optimal recovery of biological activity.
Key Features
Ready-to-Use: Pre-adjusted to pH 9.0, requiring no dilution or further preparation
Rapid Neutralization: Quickly restores physiological pH conditions following acidic elution
High Buffering Capacity: 1M concentration ensures effective neutralization of low-pH elution buffers
Preserves Antibody Integrity: Prevents prolonged acid exposure that can lead to antibody denaturation and activity loss
Wide Compatibility: Ideal for use with all acidic elution buffers including Aladdin's IgG Elution Buffer (pH 2.7)
Applications
Immediate neutralization of antibodies following Protein A/G/L affinity purification
Compatible with both manual and automated purification systems
Suitable for neutralizing eluents from various affinity chromatography methods
Ideal for research-scale and process development applications
Instructions for Use
Add Neutralization Buffer to collection tubes prior to elution (recommended volume: 1/10 of expected eluent volume)
Collect acidic eluate (e.g., from Aladdin IgG Elution Buffer, pH 2.7) directly into the buffer
Mix gently to ensure complete neutralization
Verify final pH if necessary; the mixture should reach approximately neutral pH (7.0-7.4)
Notes
For optimal antibody recovery, neutralize immediately after collection
Adjust neutralization buffer volume proportionally for larger-scale purifications
Custom pH formulations (pH 8.0-9.0) available upon request
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N1456179 | |
| 分析证书 | N1456179 | |
| 分析证书 | N1456179 | |
| 分析证书 | N1456179 | |
| 分析证书 | N1456179 | |
| 分析证书 | N1456179 |