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尼氏染色液 (甲基紫法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
神经元细胞体包括一个具有皱褶核膜的大细胞核、稀疏的染色质和一个明显的核仁，在细胞体中细胞质是尼氏颗粒，即能够代表粗面内质网并在很多神经元中产生特异的斑点状嗜碱性表现的嗜碱性颗粒。尼氏颗粒可以用很多染色来显示如中性红、亚甲基蓝、甲苯胺蓝和甲基紫等，染色的变异、pH和分化的时间使一些染色既可以仅突出尼氏物质，也可以显示神经元的细胞核和神经胶质。尼氏体 (Nissl body)或称尼氏小体是分布于神经细胞胞质内的三角形或椭圆形小块状物质，能被碱性染料如硫堇、亚甲蓝、甲苯胺蓝和焦油紫等染料染成紫蓝色；各种神经细胞内都含有尼氏体，但其形状、数量、分布位置常常不同，尼氏体也存在于树突中，但不在于轴突和胞体的轴丘。尼氏体会因为生理状态的变化而变化，尼氏体是神经元内合成蛋白质的重要部位，当神经元受到刺激后，胞体内的尼氏体会明显减少。
尼氏染色液 (Nissl Stain, 甲基紫法)主要特点是操作简便、染色稳定、分化时间短，适用范围广，可以用于石蜡组织切片的尼氏物质、神经元等的染色，尼氏体的存在和消失是神经细胞是否受损的重要指标，当发生脑炎、脑缺血、轴突反应等情况时，尼氏体会发生溶解甚至消失。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、中性福尔马林溶液、蒸馏水
2、显微镜
参考操作(仅供参考)：
1、固定: 可采用乙醇、Carnoy固定液或中性福尔马林溶液，常规脱水包埋。
2、组织切片: 石蜡切片7-10μm或25μm (见注意事项5)，切片经二甲苯或脱蜡透明液脱蜡入水。
3、用甲基紫染色液滴染，染色10-20min，蒸馏水冲洗。
4、用Nissl Differentiation分化4-8s，直到大部分染色被消除。
5、直接经无水乙醇至二甲苯或脱蜡透明液透明，显微镜下观察。
6、如果有必要，重复步骤3；重复时，给予少量Nissol Differentiation分化。
7、在二甲苯或脱蜡透明液中充分冲洗，加拿大香脂或DPX封片。
染色结果：
尼氏物质或尼氏小体: 紫黑蓝色
神经元: 淡紫蓝色
细胞核: 紫蓝色
注意事项：
1、尼氏体离体后容易溶解，所以组织取出后应立即固定，否则难以着色。
2、组织固定起着非常重要的作用，固定可采用乙醇、Carnoy固定液或中性福尔马林溶液。
3、该染色液对石蜡组织切片的尼氏染色效果较好。
4、如果要证实尼氏物质的存在，那么染色后必须要用Nissol Differentiation分化。
5、石蜡切片厚度7-10µm或25µm (皮质神经元密度的评估要用25µm厚的切片)。
6、染色后的标本务必避光保存，否则容易褪色。
7、为了您的安全和健康，请穿实验服并戴一次性手套操作。
8、试剂开封后请尽快使用，以防影响后续实验效果。
The neuronal cell body contains a large nucleus with a folded nuclear membrane, sparse chromatin, and a distinct nucleolus. Within the cell body, the cytoplasm is filled with Nissl granules-basophilic granules that represent rough endoplasmic reticulum and produce a characteristic spotted basophilic appearance in many neurons. Nissl granules can be visualized using various stains, such as neutral red, methylene blue, toluidine blue, and methyl violet. Variations in staining protocols, pH levels, and differentiation times allow some stains to either specifically highlight Nissl substance or simultaneously visualize neuronal nuclei and neuroglia. Nissl bodies, also known as Nissl substance, are triangular or oval granular structures distributed in the cytoplasm of neurons. They can be stained purple-blue by basic dyes including thionine, methylene blue, toluidine blue, and cresyl violet. All types of neurons contain Nissl bodies, but their shape, quantity, and distribution vary across different neuronal subtypes. Nissl bodies are also present in dendrites but are absent from axons and the axon hillock of the cell body. Nissl bodies undergo changes in response to the physiological state of neurons: as key sites for protein synthesis in neurons, their volume decreases significantly when neurons are stimulated.
Nissl Staining Solution (Methyl Violet Method) is characterized by simple operation, stable staining results, short differentiation time, and broad applicability. It is suitable for staining Nissl substance and neurons in paraffin-embedded tissue sections. The presence or disappearance of Nissl bodies is a critical indicator of neuronal damage. Nissl bodies may dissolve or even disappear in pathological conditions such as encephalitis, cerebral ischemia, and axonal reaction. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
Materials to Be Prepared by User
1. Neutral formalin solution, distilled water
2. Microscope
Reference Procedure (for reference only)
1. Fixation: Fix tissues using ethanol, Carnoy's fixative, or neutral formalin solution, followed by routine dehydration and embedding.
2. Tissue Sectioning: Prepare paraffin sections with a thickness of 7-10 μm or 25 μm (see Precaution 5). Dewax the sections to water using xylene or environment-friendly dewaxing clearing solution.
3. Stain the sections with Methyl Violet Staining Solution for 10-20 minutes, then rinse with distilled water.
4. Differentiate the sections with Nissl Differentiation Solution for 4-8 seconds until most of the background staining is removed.
5. Dehydrate directly through anhydrous ethanol, then clear with xylene or dewaxing clearing solution, and observe under a microscope.
6. Repeat step 3 if necessary; apply a small amount of Nissl Differentiation Solution for re-differentiation after re-staining.
7. Rinse thoroughly in xylene or dewaxing clearing solution, then mount the sections with Canada balsam or DPX mounting medium.
Staining Results
Nissl substance or Nissl bodies: Purple-black-blue
Neurons: Pale purple-blue
Cell nuclei: Purple-blue
Precautions
1. Nissl bodies are prone to dissolution post-mortem. Tissues must be fixed immediately after dissection; otherwise, Nissl bodies cannot be stained effectively.
2. Tissue fixation plays a crucial role in staining success. Ethanol, Carnoy's fixative, or neutral formalin solution are recommended fixatives.
3. This staining solution yields optimal results for Nissl staining of paraffin-embedded tissue sections.
4. To confirm the presence of Nissl substance, differentiation with Nissl Differentiation Solution is mandatory after staining.
5. Prepare paraffin sections with a thickness of 7-10 μm or 25 μm (25 μm-thick sections are required for evaluating the density of cortical neurons).
6. Stained specimens must be stored in the dark to prevent fading.
7. For your safety and health, please wear a lab coat and disposable gloves during operation.
8. Please use the reagent as soon as possible after opening to avoid compromising subsequent experimental results.
规格
别名
尼氏染色液
英文别名
Nissl Staining Solution
规格或纯度
BioReagent,用于显微镜,生物染色剂
英文名称
Nissl Staining Solution (Methyl Violet Method)
应用
细胞染色
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
各组分在相应的储存条件下保质期1年。
组分与储存
| N1508447 | Component | 2×50 mL | Storage |
| N1508447A | 甲基紫染色液 | 50 mL | RT. Store in the dark. |
| N1508447B | Nissl Differentiation | 50 mL | RT. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332877 |
分析证书 | N1508447 |
技术文档和文章
溶液计算器
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