计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
硝酸还原酶(NR,EC 1.7.1.3)广泛存在于植物中,是植物硝态氮转化为氨态氮的关键酶,也是诱导酶,对作物的产量和品质有影响。本试剂盒能够检测植物组织、细菌或细胞样本中硝酸还原酶活性,其原理是 NR 催化硝酸盐还原为亚硝酸盐,NO₃⁻ + NADH + H⁺ → NO₂⁻ + NAD⁺ + H₂O,产生的亚硝酸盐能够在酸性条件下,与对–氨基苯磺酸及α-萘胺定量生成红色偶氮化合物,生成的红色偶氮化合物在 540 nm 有最大吸收峰,可用分光光度法测定。
Nitrate reductase (NR, EC 1.7.1.3) is widely present in plants. It is a key and inducible enzyme that catalyzes the conversion of nitrate nitrogen to ammonium nitrogen in plants, influencing crop yield and quality. This kit can detect nitrate reductase activity in plant tissues, bacterial or cellular samples. The principle is that NR catalyzes the reduction of nitrate to nitrite: NO₃⁻ + NADH + H⁺ → NO₂⁻ + NAD⁺ + H₂O. The generated nitrite can quantitatively form a red azo compound with sulfanilic acid and α-naphthylamine under acidic conditions. This red azo compound exhibits a maximum absorption peak at 540 nm, allowing for detection by spectrophotometry.