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硝酸还原酶（NiR）是一类能催化亚硝酸盐还原的酶，广泛存在于微生物及植物体内，是自然界氮循环过程中的关键酶，可以将亚硝酸盐降解为NO或NH₃，从而减少环境中亚硝态氮的积累，降低因亚硝酸盐累积而造成的对生物体的毒害作用。 测定原理：亚硝酸还原酶可将NO₂⁻还原为NO，使样品中参与重氮化反应生成紫红色化合物的NO₂⁻减少，即540nm处吸光值的变化可反应亚硝酸还原酶的活性。
Nitrite Reductase (NiR) is an enzyme capable of catalyzing the reduction of nitrite. It is widely present in microorganisms and plants and serves as a key enzyme in the natural nitrogen cycle. By degrading nitrite into NO or NH₃, NiR helps reduce the accumulation of nitrite nitrogen in the environment, thereby mitigating the toxic effects of nitrite buildup on organisms. Assay Principle: Nitrite reductase reduces NO₂⁻ to NO, decreasing the amount of NO₂⁻ available to participate in the diazotization reaction that produces a purple-red compound. Thus, the change in absorbance at 540 nm reflects the activity of nitrite reductase.
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