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硝酸还原酶(Nitrate reductase，NR)是一种氧化还原酶，可分为参与硝酸盐同化的同化型还原酶和催化以硝酸盐为活体氧化的最终电子受休的硝酸盐呼吸异化型(呼吸型)还原酶。硝酸还原酶是植物氮素代谢中氮素同化的关键酶，该酶与作物吸收利用氮肥有关，对作物的产量和质量有影响，因此可以把硝酸还原酶的活力当作营养诊断、农田施肥或作物育种的生理生化指标。硝酸还原酶(NR)测定方法可分为活体法和离体法，活体法步骤简，适合快速、多组测定；离体法比较复杂，但重复性好。 该试剂盒检测原理是硝酸还原酶催化硝酸盐还原为亚硝酸盐，其反应如下：NO₃⁻ + NADH + H⁺ → NO₂⁻ + NAD⁺ + H₂O，产生的亚硝酸盐与磺胺及萘胺在酸性条件下定量生成稳定的红色偶氮化合物，于酶标仪520~540nm处检测吸光度，由产生的亚硝态氮的量表示硝酸还原酶的活性，一般以单位μg/(g•h)表示，主要用于检测植物样本中硝酸还原酶活性。该产品仅用于科研领域，不适用于临床诊断或其他用途。
Nitrate Reductase (NR) is an oxidoreductase enzyme. It can be categorized into assimilatory reductases involved in nitrate assimilation for nitrogen anabolism and dissimilatory (respiratory) reductases that catalyze nitrate respiration, using nitrate as the final electron acceptor for anaerobic oxidation. Nitrate reductase is a key enzyme in nitrogen assimilation within plant nitrogen metabolism. This enzyme is related to the crop's ability to absorb and utilize nitrogen fertilizer, influencing both crop yield and quality. Therefore, nitrate reductase activity can be used as a physiological and biochemical indicator for nutritional diagnosis, field fertilization strategies, or crop breeding programs. Methods for determining Nitrate Reductase (NR) activity can be divided into in vivo assays and in vitro assays. The in vivo method involves simple steps and is suitable for rapid, multi-sample determination; the in vitro method is more complex but offers better reproducibility. The detection principle of this kit is as follows: Nitrate reductase catalyzes the reduction of nitrate to nitrite, according to the reaction: NO₃⁻ + NADH + H⁺ → NO₂⁻ + NAD⁺ + H₂O. The nitrite produced reacts quantitatively with sulfanilamide and naphthylamine under acidic conditions to form a stable red azo compound. The absorbance is measured at 520-540 nm using a microplate reader. Nitrate reductase activity is expressed by the amount of nitrite nitrogen produced, typically in units of μg/(g•h). This kit is primarily used for detecting nitrate reductase activity in plant samples. This product is for research use only and is not intended for use in diagnostic procedures.
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