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BioReagent,适用于微生物学 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
室温。常规运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
LB 是分子生物学最广泛使用的细菌培养基,可用于大肠杆菌、恶臭假单胞菌、副溶血性弧菌、芽孢杆菌等细 菌的培养,配制简单,营养丰富,含有多肽、氨基酸、水溶性维生素和碳水化合物,可用于基因克隆、质粒 DNA 生产和重组蛋白生产等实验。非动物源 LB 是采用精炼非动物源蛋白胨和酵母粉开发的用于大肠杆菌培养的 LB 培 养基,可用于有非动物源培养基要求的试验 (疫苗、药用质粒、药用蛋白等的生产),用于 DH5a 等克隆菌株培养时,菌体产量和质粒产量比普通 LB 高出约 30%;用于 BL21 DE3 等原核表达菌株培养时菌体产量比普通 LB 高出 20%-30%。
产品组分:
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使用方法:
非动物源 LB with Agar 粉剂培养基:取一袋 (20g),加双蒸水 450ml,待 Tryptone,NaCl,Yeast Extract 溶解后 (Agar 不溶解),定容到 0.5L,121℃ 高压灭菌 20min,灭菌后温度降到 55 ℃ 以下使用,或放室温保存。
注意事项:
1、LB 培养基需先补水,121℃ 高压灭菌 20min 后使用。
2、若发现有严重吸潮现象,停止使用。
3、LB 培养基加入 pH=7.0 的去离子水后 pH 约 7.0,可不调 pH 值直接使用。
4、若配制少于 0.5L,按每 100ml 加 2.5g 培养基的比例加入即可,剩余培养基封口后放干燥处保存。
LB medium is the most widely used bacterial culture medium in molecular biology. It can be applied to the culture of Escherichia coli, Pseudomonas putida, Vibrio parahaemolyticus, Bacillus and other bacteria. It is easy to prepare and rich in nutrients, containing peptides, amino acids, water-soluble vitamins and carbohydrates, and suitable for experiments such as gene cloning, plasmid DNA preparation and recombinant protein production.Animal-Free LB is an LB medium developed with refined animal-free peptone and yeast extract for culturing E. coli. It meets experimental standards requiring animal-component-free media, including the production of vaccines, pharmaceutical plasmids and medicinal proteins. When culturing cloning strains such as DH5α, it achieves approximately 30% higher biomass and plasmid yields than standard LB medium. For prokaryotic expression strains including BL21 (DE3), its cell output is 20%–30% higher than conventional LB medium.
Product Composition:
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Instructions for Use:
Animal-Free LB with Agar Powder Medium: Take one pouch (20 g) and add to 450 mL double-distilled water. Let animal-free tryptone, NaCl and yeast extract dissolve thoroughly (agar will not dissolve), then make up the volume to 0.5 L. Autoclave at 121°C for 20 minutes. Cool the sterilized medium to below 55 °C prior to use, or store it at room temperature.
Precautions
1. LB medium shall be mixed with water first and autoclaved at 121 °C for 20 minutes before use.
2. Stop using if the powder suffers severe moisture absorption.
3. Dissolving LB medium in pH 7.0 deionized water results in a pH of approximately 7.0, so no pH adjustment is required before use.
4. For preparation volumes less than 0.5 L, add 2.5 g medium powder per 100 mL water. Seal the residual powder tightly and store in a dry environment.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N1524574 |
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