Green Stain (10000×) 是一种基于聚集诱导发光荧光材料的三苯胺衍生物，具有典型的 AIE 特性。产品具有较宽的激发光谱特性，可以在不改变任何成像系统的情况下，替换溴化乙锭（EB）和其它凝胶染料，是与激光扫描仪配合使用的理想选择。
【产品特性】
Green Stain 是一种灵敏度高、稳定性强、适用性广且极具兼容性的核酸凝胶染料，可用于对琼脂糖凝胶和聚丙烯酰胺凝胶中的 dsDNA，ssDNA 进行染色。其灵敏度较好，检测限低于 1 ng。在胶染色方面，是银染的理想替代品，在 10 min 内即可获得清晰明亮的条带，操作简单，结果直观。并且 Green Stain 具有较宽的激发光谱特性，因此极具兼容性，可以在不改变任何成像系统的情况下用来替换溴化乙锭（EB）和其它凝胶染料，适用于原先使用 SYBR Green 或 SYBR Gold 等为核酸染料的凝胶成像和检测系统，这可使其成为与激光扫描仪配合使用的理想选择。并且，其可以使用对人体无害的蓝光灯或蓝光成像仪进行核酸检测，从而避免常规的紫外检测对核酸样品的致突变性，以及紫外对人的眼睛和皮肤的伤害。
【产品优势】
(1) 灵敏度高：适用于各种大小片段的核酸电泳染色，灵敏度＜1 ng；
(2) 兼容性广：通道匹配标准凝胶成像系统以及可见光激发的凝胶观察装置；
(3) 快速简单：染料分子与核酸分子结合速率快，泡染 10 min 内即可获得清晰明亮的条带；
(4) 光稳定性强：自然光下曝光，多次拍摄，其荧光强度不会衰减；
(5) 染色方式灵活：前染法、点染法、泡染法效果均非常好；
(6) 适用性广：大小片段均适用，琼脂糖凝胶和聚丙烯酰胺凝胶均适用；
(7) 安全性好：几乎不能穿透细胞膜，安全性高，且无致突变性和细胞毒性。
【实验方法】
一、胶染法（前染法）（用法同 EB，推荐）
1．按常规操作，制备琼脂糖凝胶，加热至胶完全溶解后，加入浓缩的 10000× Green Stain，使其在凝胶中的终浓度为 1× （比如，制备 100mL 凝胶，加入染料 10μL，可根据实际情况调整用量），轻轻摇匀，倒胶。
2．按常规方法电泳，观测结果。
二、点染法
1．按常规操作，制备琼脂糖凝胶，待胶凝固后，将染料与样品及 loading buffer 进行混匀，后加载到上样孔中（不影响迁移速率）。
2．按常规方法电泳，观测结果。
三、泡染法（后染法）
1. 按照常规方法进行电泳。
2. 用 ddH₂O 将 10000× Green Stain 浓缩液稀释约 2000 倍到 0.1M 的 NaCl 中，制成 5× 染色液。（比如，将 10μL 10000× Green Stain 浓缩液和 5mL 1M NaCl 加到 45mL ddH₂O 中）。
3. 将凝胶小心放入合适的容器中，缓慢加入足量的 5× 染色液浸没胶。室温振荡染色约 10min 即可，最佳染色时间根据凝胶厚度及琼脂糖浓度不同而略有不同。对于 3.5-10% 丙烯酰胺胶，染色时间可以适当延长。然后观测结果。
【注意事项】
1、使用前请先短暂混匀；
2、为了您的安全和健康，请穿实验服并戴一次性手套操作；
3、本品仅适用于科研用途。
Green Stain (10000×) is a triphenylamine derivative based on aggregation-induced emission fluorescent materials and exhibits typical AIE properties. Featuring a broad excitation spectrum, this product can replace ethidium bromide (EB) and other gel stains without modifying any imaging system, making it an ideal match for laser scanners.
Product Features
Green Stain is a high-sensitivity, stable, versatile and fully compatible nucleic acid gel stain for staining double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) in agarose and polyacrylamide gels. It delivers excellent sensitivity with a limit of detection below 1 ng. As an ideal alternative to silver staining, it generates clear, bright bands within 10 minutes via straightforward procedures with intuitive results. Thanks to its broad excitation spectrum, Green Stain boasts outstanding compatibility. It can substitute ethidium bromide (EB) and other gel stains without hardware adjustments, and works seamlessly with gel imaging and detection systems originally designed for nucleic acid stains such as SYBR Green or SYBR Gold, rendering it perfect for use with laser scanners. Moreover, nucleic acid detection can be performed using blue light transilluminators or blue light imaging devices that pose no harm to humans. This avoids the mutagenic damage UV radiation inflicts on nucleic acid samples, as well as UV-induced injuries to human eyes and skin.
Product Advantages
1. High sensitivity: Suitable for electrophoretic staining of nucleic acid fragments of all sizes; detection sensitivity < 1 ng;
2. Wide compatibility: Matches standard gel imaging systems and visible light-excited gel viewing equipment;
3. Fast & simple operation: Rapid binding between dye and nucleic acid molecules; distinct bright bands obtained within 10 minutes of gel soaking;
4. Excellent photostability: Fluorescence intensity remains unchanged under natural light exposure and repeated image capture;
5. Flexible staining modes: Pre-gel staining, sample co-staining and post-gel soaking all yield excellent results;
6. Broad applicability: Compatible with all fragment sizes, as well as both agarose and polyacrylamide gels;
7. Superior safety: Barely penetrates cell membranes; non-mutagenic and non-cytotoxic.
Experimental Protocols
I. Pre-Gel Staining (Pre-Stain, Recommended; Operation Identical to EB)
1. Prepare agarose gel following standard protocols. After complete gel dissolution by heating, add concentrated 10,000× Green Stain to reach a final concentration of 1× in the gel. Example: Add 10 μL of concentrated stain to 100 mL gel solution; dosage can be adjusted as needed. Mix gently and pour the gel.
2. Perform electrophoresis per standard procedures and visualize results.
II. Sample Co-Staining (Loading Well Staining)
1. Prepare agarose gel routinely. Once solidified, mix the stain with samples and loading buffer before loading the mixture into sample wells (no impact on nucleic acid migration rate).
2. Run electrophoresis and observe bands as standard.
III. Post-Gel Soaking Staining
1. Complete electrophoresis following standard protocols.
2. Dilute 10,000× concentrated Green Stain approximately 2,000-fold in 0.1 M NaCl to prepare 5× working staining solution. Preparation example: Add 10 μL 10,000× concentrated stain and 5 mL 1 M NaCl into 45 mL ddH₂O.
. Place the gel gently into a suitable container and add sufficient 5× staining solution to fully submerge the gel. Shake at room temperature for roughly 10 minutes; optimal staining time varies slightly with gel thickness and agarose concentration. Extend staining time appropriately for 3.5–10% polyacrylamide gels. Visualize bands afterward.
Precautions
1. Briefly vortex the product before use.
2. Wear lab coats and disposable gloves during operation for personal safety.
3. This product is for research use only.
ZJ26F0838574
危险品化学品经营许可证（带存储）