计算溶液所需的质量、体积或浓度。
非冻型组织RNA保存液
有货
为什么选择此级别
BioReagent,分子生物学级,即用型 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品是一种液态的，无毒的组织 RNA 保存试剂，能迅速渗入组织抑制 RNAase，稳定并保护组织样品中的 RNA。本产品应用范围广，适用于多种动物组织（如肝脏、脑、肺、脾脏、肾脏、肌肉组织等），还可用于酵母、细菌、真菌、细胞以及某些植物样品的 RNA 保存。经本产品浸润的组织或细胞，可在 37℃保存 1 天，室温保存 1 周，2℃-8℃保存 4 周，-20℃/-80℃长期保存。本产品几乎兼容所有的 RNA 分离方法，如 Trizol 法、离心柱法和磁珠纯化法等。
使用说明
（一）样品处理
1. 动物组织样品：将组织剪切成长宽高均不大于 0.5 厘米的组织块，迅速加入 5-10 倍体积的非冻型组织RNA保存液。注意如骨头等液体渗透性较弱的组织，不适合用本品进行 RNA 保护。
2. 细胞样品：收集细胞沉淀，用 PBS 洗涤后，5000g 离心 1-2 分钟，吸除 PBS 后小心加入 5-10 倍体积的非冻型组织RNA保存液，避免吹起细胞。
3. 细菌样品：12000g 离心 2 分钟，弃上清加入 5-10 倍体积非冻型组织RNA保存液，对酵母和真菌样品进行保存时，请参考细菌处理方法。
（二）样品保存
1. 浸透于试剂中的样品在 37℃可保存一天，室温保存一周，2-8℃保存一个月。
2. -20℃保存方法：样本 4℃浸透过夜，然后转移至-20℃长期保存，试剂不会在-20℃冻
结，但有可能出现结晶。可直接放在室温融化，不会影响 RNA 的提取量和完整性。
3. -80℃保存方法：样本 4℃浸透过夜，然后转移至-80℃长期保存（建议样品在转移至-80℃前将其从试剂中取出，直接冻于-80℃保存）。样本可直接放在室温融化，再重新冻上，不会影响 RNA 的提取量和完整性。
（三）RNA 提取
1. 组织样品：用干净的镊子从试剂中取出组织块，吸水纸吸掉表面液体，之后立即置于裂解液中匀浆或液氮中研磨。
2. 细胞和细菌等样品：加入与试剂等体积的 DEPC 处理水，10000g 离心 1 分钟收集样品，小心去除上清，后续即可用各种方法提取 RNA。酵母和真菌参考本方法提取 RNA。
注意事项
1. 本产品除保护 RNA 外，也可保护样品中的 DNA 和蛋白质，但会导致蛋白质变性，不适用于天然蛋白质的提取。
2. 本产品仅适用于新鲜组织样品或细胞样品。不可用于冰冻组织或细胞。
3. 经本产品保存的样品，会变得微硬，为正常现象。
4. 本产品存放过程中可能会出现沉淀，使用前放到 37℃水浴中加热，沉淀溶解后不影响使用。
5. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
This product is a liquid, non-toxic tissue RNA preservation reagent that rapidly penetrates tissues to inhibit RNase activity, stabilizing and protecting RNA in tissue samples. This product has a wide range of applications and is suitable for various animal tissues (such as liver, brain, lung, spleen, kidney, muscle tissue, etc.), as well as for RNA preservation in yeast, bacteria, fungi, cells, and certain plant samples. Tissues or cells infiltrated with this product can be stored at 37℃ for 1 day, at room temperature for 1 week, at 2℃-8℃ for 4 weeks, and long-term at -20℃/-80℃. This product is compatible with almost all RNA isolation methods, such as Trizol method, spin-column method, and magnetic bead purification method.
Instructions for Use
(I) Sample Processing
1.Animal Tissue Samples: Cut the tissue into pieces with dimensions no greater than 0.5 cm in each direction, and immediately add 5-10 volumes of Non-frozen Tissue RNA Preservation Solution. Note: Tissues with poor liquid permeability, such as bone, are not suitable for RNA protection with this product.
2.Cell Samples: Collect cell pellets, wash with PBS, centrifuge at 5000g for 1-2 minutes, remove the PBS, and carefully add 5-10 volumes of Non-frozen Tissue RNA Preservation Solution without resuspending the cells.
3.Bacterial Samples: Centrifuge at 12000g for 2 minutes, discard the supernatant, and add 5-10 volumes of Non-frozen Tissue RNA Preservation Solution. For preservation of yeast and fungal samples, refer to the bacterial processing method.
(II) Sample Storage
1.Samples immersed in the reagent can be stored at 37℃ for 1 day, at room temperature for 1 week, and at 2-8℃ for 1 month.
2.-20℃ Storage: Infiltrate samples overnight at 4℃, then transfer to -20℃ for long-term storage. The reagent will not freeze at -20℃, but crystallization may occur. It can be thawed directly at room temperature without affecting RNA yield or integrity.
3.-80℃ Storage: Infiltrate samples overnight at 4℃, then transfer to -80℃ for long-term storage (it is recommended to remove samples from the reagent before transferring to -80℃ and freeze them directly at -80℃). Samples can be thawed directly at room temperature and re-frozen without affecting RNA yield or integrity.
(III) RNA Extraction
1.Tissue Samples: Use clean forceps to remove tissue pieces from the reagent, blot off excess liquid with absorbent paper, and immediately place into lysis buffer for homogenization or grind in liquid nitrogen.
2.Cell and Bacterial Samples: Add an equal volume of DEPC-treated water to the reagent, centrifuge at 10000g for 1 minute to collect the sample, carefully remove the supernatant, and proceed with various RNA extraction methods. For yeast and fungi, refer to this method for RNA extraction.
Precautions
1.This product not only protects RNA but also preserves DNA and proteins in the sample. However, it causes protein denaturation and is not suitable for the extraction of native proteins.
2.This product is only applicable to fresh tissue or cell samples. Do not use on frozen tissue or cells.
3.Samples preserved with this product may become slightly hardened, which is a normal phenomenon.
4.Precipitation may occur during storage of this product. Before use, warm in a 37℃ water bath; the precipitate will dissolve and does not affect performance.
5.For your safety and health, please wear a lab coat and disposable gloves when handling.
规格
规格或纯度
BioReagent, 即用型, 分子生物学级
稳定性与储存
Store at 2-8°C long term (24 months).
英文名称
Non‑frozen Tissue RNA Preservation Solution
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
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