计算溶液所需的质量、体积或浓度。
Neuraminidase Au
有货
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Product Description
α (2-3,6,8,9) Neuraminidase Au cleaves all cleaves all non-reducing terminal sialic acid residues from complex carbohydrates and glycoproteins. The relative cleavage rates for different linkages are:α(2-6) > α(2-3) > α(2-8), α(2-9).
In addition, the enzyme will cleave branched sialic acids (linked to an internal residue). This property makes it unique among neuraminidases. High concentrations of enzymes and prolonged incubation times may be required for cleaving branched residues. To cleave only non- reducing terminal α(2-3) unbranched sialic acid residues, use Neuraminidase SP, part number E-S007.
α-(2-3,6,8,9) Neuraminidase is isolated from a clone of Arthrobacter ureafaciens. The enzyme has been extensively characterized using oligosaccharide standards.
Contents
Neuraminidase in 20 mM Tris-HCl, 25 mM NaCl, pH 7.5
Included with 20 µL and 60 µL pack sizes:
5x Reaction Buffer 250 mM sodium phosphate, pH 6.0
Molecular weight
~69,000 daltons
pH optimum
6.0, active over the range 4.5-7
50 mM sodium phosphate (pH 6.0) provides the optimal
buffer for enzyme activity with sialyllactose, a standard
substrate. If glycosidase treatment is performed at
suboptimal pH because of glycoprotein solubility or
activity requirements, expect some diminution in enzyme
activity.
Specificity
All non-reducing terminal branched and unbranched sialic acid.
Formulation
The enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
Specific Activity Assay
One unit of Neuraminidase Au is defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA (2’.-(4-methyl-umbelliferyl)-alpha-D-N acetylneuraminic acid].
Stability
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Purity
Neuraminidase Au is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strain has been extensively tested and does not produce any detectable glycosidases.
Directions for use
1. Add up to 100 µg of glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add de-ionized water to a total of 14 µl.
3. Add 4 µl Reaction Buffer 6.0.
4. Add 2 µl Neuraminidase Au.
5. Incubate at 37°C for 1 hour. NOTE: longer incubation times are necessary if branched sialic acids are present.
Desialylation may be monitored by SDS-PAGE if the size differential between native and de-sialylated protein is sufficient for detection.
NOTE: longer incubation times are necessary if branched sialic acids are present.
规格
别名
神经氨酸酶金
英文别名
Sialidase, NANase, N-acetylneuraminate glycohydrolase
规格或纯度
Specific Activity >135 U/mg;Activity>5 U/ml
英文名称
Neuraminidase Au
来源
recombinant from Arthrobacter ureafaciens in E. Coli
储存条件
2-8°C储存
运输条件
低温运输
产品线
生物产品
名称和识别符
酶学委员会编号
3.2.1.18
分子类型
酶
技术文档
高级数据
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C of A & Other Certificates(BSE/TSE, COO):
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技术文档和文章
溶液计算器
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