计算溶液所需的质量、体积或浓度。
概述
Product Description
α(2-3) Neuraminidase Sp cleaves exclusively the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α(2-6) or α(2-8) linkages or on branched α(2-3) linkages . To cleave all non-reducing terminal sialic acid residues including branched sialic acids (linked to an internal residue) from complex carbohydrates and glycoproteins, use α(2-3,6,8,9) Neuraminidase Au.
Contents
Neuraminidase Sp in 50 mM sodium phosphate, pH 7.5
Included with 20 µL and 60 µL pack sizes:
5x Reaction Buffer 250 mM sodium phosphate, pH 6.0
Specificity
All non-reducing terminal branched and unbranched a-(2- 3) sialic acid.
Specific Activity Assay
One unit of Neuraminidase is defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37°C, pH 5.0 from MU-NANA (2’.-(4-methyl-umbelliferyl)-alpha-D-N acetylneuraminic acid].
Molecular Weight
~75,000 daltons
pH optimum
6.0, active over the range 4.5-7.
50 mM sodium phosphate (pH 6.0) provides the optimal buffer for enzyme activity with sialyllactose, a standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.
Formulation
The enzyme is provided as a sterile-filtered solution in in 50 mM Sodium phosphate pH 7.5.
Stability
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
Purity
Neuraminidase Sp is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strain has been extensively tested and does not produce any detectable glycosidases.
Directions for use
1. Add up to 100 µg of glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add de-ionized water to a total of 14 µl.
3. Add 4 µl 5x Reaction Buffer 6.0.
4. Add 2 µl Neuraminidase Sp.
5. Incubate at 37°C for 1 hour.
NOTE: longer incubation times are necessary if branched sialic acids are present.
Desialylation may be monitored by SDS-PAGE if the size differential between native and de-sialylated protein is sufficient for detection.
Applications
•Structural analysis of oligosaccharides
•Determining sialic acid linkage
•Glycoprotein deglycosylation
•Removing heterogeneity from glycoproteins
规格
产品名称
Neuraminidase Sp
别名
神经氨酸酶
英文别名
Sialidase, NANase, N-acetylneuraminate glycohydrolase
规格或纯度
Specific Activity ≥ 250 U/mg;Activity ≥ 10 U/ml
酶学委员会编号
3.5.1.18
分子类型
酶
产品线
生物产品
储存与运输
浓度
Specific Activity ≥ 250 U/mg;Activity ≥ 10 U/ml
储存条件
2-8°C储存
运输条件
低温运输
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 SSE:688179 保留所有权利。
危险品化学品经营许可证（带存储）