计算溶液所需的质量、体积或浓度。
| 活性类型 | 活性值-log(M) | 作用机制 | 期刊 | 参考文献(PubMed IDs) |
|---|
为什么选择此级别
提供 ≥98% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-80℃储存。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
Norartocarpetin is a tyrosinase inhibitor. Norartocarpetin has strong tyrosinase inhibitory activity with an IC 50 value of 0.47 μM. Norartocarpetin as an antibrowning agent can be used for the research of food systems. Norartocarpetin also has a significant anticancer activity in lung carcinoma cells (NCI-H460) with an IC 50 value of 22 μM. Norartocarpetin has antiproliferative effects are mediated via targeting Ras/Raf/MAPK signalling pathway, mitochondrial mediated apoptosis , S-phase cell cycle arrest and suppression of cell migration and invasion in human lung carcinoma cells
In Vitro
Norartocarpetin has strong mushroom tyrosinase inhibitory activity with an IC 50 value of 0.47 μM. Norartocarpetin (0-100 μM; 48 h) leds to dose-dependent cytotoxic effects in NCI-H460 cells and MRC-9 cells with IC 50 values of 22 μM and 85 μM, respectively. Norartocarpetin (0, 11, 22, and 44 μM; 24 h) led to blockade of Ras/Raf/MAPK signalling pathway. Norartocarpetin (0, 11, 22, and 44 μM, 24 h) induced the apoptosis of the human lung carcinoma cells (NCI-H460). Norartocarpetin (0, 11, 22, and 44 μM) leds to S-phase cell cycle arrest. Norartocarpetin (22 μM; 24 h) leds to dose-dependent suppression of cell invasion. Norartocarpetin (0, 11, 22, and 44 μM, 24 h) leds to significant inhibition of cell migration. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: MRC-9 (normal fibroblast lung cells) and NCI-H460 (human lung cancer cells) Concentration: 0, 3.12, 6.25, 12.5, 25, 50, and 100 μM (first dissolved in DMSO at 1.0 mg/mL and then diluted to different concentrations using DMSO, 30 μL, 20min) Incubation Time: 48 h Result: Repressed the viability of NCI-H460 cells in a dose-dependent manner. Western Blot AnalysisCell Line: MRC-9 and NCI-H460 cells Concentration: 0, 11, 22, and 44 μM Incubation Time: 24 h Result: Significantly reduced the expression of p-RAS, p-RAF and p-P38 and unchanged the expressions of RAS, RAF and P38. Apoptosis AnalysisCell Line: MRC-9 and NCI-H460 cells Concentration: 0. 11, 22 and 44 μM Incubation Time: 24 h Result: Led to dose-dependent apoptosis and induced DNA damage. Cell Cycle AnalysisCell Line: MRC-9 and NCI-H460 cells Concentration: 0, 11, 22, and 44 μM Incubation Time: Result: Showed increase of S-phase cells and the percentage of S-phase NCI-H460 LC cells was 30%, 40%, 55% and 70% at 0, 11, 22 and 44 μM concentrations, respectively. Cell Invasion AssayMRC-9 and NCI-H460 cells Cell Line: MRC-9 and NCI-H460 cells Concentration: 22 μM Incubation Time: 24 h Result: Significantly reducted (from 100% to about 40%) in the invasion of NCI-H460 human LC cells. Cell Migration AssayCell Line: MRC-9 and NCI-H460 cells Concentration: 0, 11, 22, and 44 μM Incubation Time: 24 h Result: Decreased remarkably the cell invasion in NCI-H460 LC cells in a concentration- dependent manner.
Form:Solid
IC50& Target:IC50: 0.47 μM (tyrosinase), IC50: 22 μM (NCI-H460 cells),85 μM (MRC-9 cells)
规格
别名
诺拉托卡佩丁
规格或纯度
≥98%
英文名称
Norartocarpetin
生化机理
Norartocarpetin 是一种酪氨酸酶抑制剂。Norartocarpetin 具有很强的酪氨酸酶抑制活性，其 IC 50 值为 0.47 μM。Norartocarpetin 作为一种抗褐变剂，可用于食品体系的研究。Norartocarpetin 还具有显著的
储存条件
避光,-80℃储存
运输条件
特低温运输
作用类型
抑制剂
纯度
≥98%
名称和识别符
分子类型
小分子
Isomeric SMILES
C1=CC(=C(C=C1O)O)C2=CC(=O)C3=C(C=C(C=C3O2)O)O
PubChem CID
分子量
286.24
技术文档
高级数据
关联靶点（人）
TYR
Tclin
酪氨酸酶(Tyrosinase)
(1 活性数据)
TYR
Tclin
Tyrosinase
(717 活性数据)
PTGS1
Tclin
Cyclooxygenase-1
(9233 活性数据)
PTGS2
Tclin
Cyclooxygenase-2
(13999 活性数据)
PTPN12
Tchem
Protein-tyrosine phosphatase G1
(132 活性数据)
BACE1
Tchem
Beta-secretase 1
(15641 活性数据)
PTPN22
Tchem
Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP
(1215 活性数据)
关联靶点（其它种属）
Gusb
Beta-glucuronidase (17 活性数据)
Ptpn1
Protein-tyrosine phosphatase 1B (270 活性数据)
RAW264.7 (28094 活性数据)
ptpA
Probable low molecular weight protein-tyrosine-phosphatase (435 活性数据)
PNLIP
Pancreatic triacylglycerol lipase (476 活性数据)
ptbB
Phosphotyrosine protein phosphatase (409 活性数据)
作用机制
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
化学和物理性质
溶解性
DMSO : 100 mg/mL (349.36 mM; Need ultrasonic)
分子量
286.240 g/mol
XLogP3
1.400
氢键供体数Hydrogen Bond Donor Count
4
氢键受体数Hydrogen Bond Acceptor Count
6
可旋转键计数Rotatable Bond Count
1
精确质量Exact Mass
286.048 Da
单同位素质量Monoisotopic Mass
286.048 Da
拓扑极表面积Topological Polar Surface Area
107.000 Ų
重原子数Heavy Atom Count
21
形式电荷Formal Charge
0
复杂度Complexity
447.000
同位素原子数Isotope Atom Count
0
定义的原子立体中心计数Defined Atom Stereocenter Count
0
未定义的原子立体中心计数Undefined Atom Stereocenter Count
0
定义的键立体中心计数Defined Bond Stereocenter Count
0
未定义的键立体中心计数Undefined Bond Stereocenter Count
0
所有立体化学键的总数The total count of all stereochemical bonds
0
共价键合单元计数Covalently-Bonded Unit Count
1
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
溶液计算器
常见问题
本产品的纯度是多少？
本产品的化学纯度（含量）为 ≥98%。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?80 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品采用干冰配冷袋冷冻运输。收货后请立即拆包，并按上述储存条件保存；处理干冰时请戴隔热手套并保持通风。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 520-30-9，分子式为 C15H10O6，分子量为 286.24 g/mol。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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