计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,生物活性,重组,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-N-Acetylglucosaminidase, sometimes called hexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-D-glucosaminide. β-N-actylglucosaminidase hydrolyzes the terminal nonreducing N-acetyl-D-hexosamine residues. This enzyme contains two predominant isozymes, Hex A, a heterodimer, and Hex B, a homodimer. N-acetylglucosamine, acetamide, N-2-acetamido-2-deoyglucosylamine, N-acetylnojirimycin, and N-acetyldeoxynojirmycin are known inhibitors.
Applications
β-N-Acetylglucosaminidase is an enzyme involved in the hydrolysis of terminal N-acetyl-D-hexosamine residues in N-acetyl-β-D-hexosaminides.
Component List
| N755074 | Component | 50U | Storage |
| N755074A | β-N-Acetylglucosaminidase (4000 U/ml) | 12.5μl | -20℃. Avoid freeze/ Thaw cycle. |
| N755074B | 10× GlycoBuffer 1 | 500μl | -20℃. Avoid freeze/ Thaw cycle. |
Reaction Conditions
1× GlycoBuffer 1
Incubate at 37°C
1× GlycoBuffer 1:
5 mM CaCl₂, 50 mM sodium acetate (pH 5.5 @ 25°C)
Product Notes
1. Reactions may be scaled-up linearly to accommodate larger reaction volumes.
2. The amount of exoglycosidase enzyme required varies when different substrates are used. Start with 1–2 μl for 1 μg of glycoprotein or 100 nM of oligosaccharide for one hour in a 10–25 μl reaction. If there is still undigested material, let the reaction go overnight.
3. This product cannot be heat inactivated.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | N755074 |
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