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S-100蛋白 来源于牛脑
有货
概述
S‑100（S100）蛋白是一类小分子钙结合蛋白，最早从脑组织中分离得到，参与调控细胞信号传导与蛋白相互作用。
阿拉丁供应牛脑纯化 S‑100 蛋白，冻干制剂，经 SDS‑PAGE 检测纯度≥95%。S-100蛋白 来源于牛脑为αα、αβ、ββ 亚型的混合物。该产品可用于 S100 免疫检测方法开发、抗体验证以及钙结合蛋白相关研究。
Biological Significance: S-100 is a low molecular weight, cytoplasmic, calcium binding protein. It is traditionally found in the brain, heart and muscle tissue. S-100 has two subunits (alpha-chain and beta-chain) and exists in several different forms. The alpha-alpha homodimer is referred to in literature as either the S-100αα or S-100a(0), the beta-beta homodimer is referred to in literature as S-100b and the alpha-beta heterodimer is referred to in literature as S-100a. ART purifies a mixture of all forms (S-100), S-100αα (<5% beta chain contamination by Western Blot), and S-100b (<10% alpha chain contamination by Western Blot).
References:
Neurochemical Research 13, 561-565 (1988); J Biol Chem 261, 8204-8212 (1986); FEBS Lett 148, 231-234 (1982); Biochem Biophys ACTA 494, (1977), 222-232; J Biol Chem 246. 6276-6283 (1971)
Prepared from tissue collected in the contiguous United States from animals under 30 months of age at a USDA-inspected facility. Animals were inspected ante mortem and found free from obvious signs of infectious and contagious diseases.
Country of Origin: US
规格
产品名称
S-100蛋白 来源于牛脑
规格或纯度
生物活性, 天然, 冻干粉, ≥95%(SDS-PAGE), 冻干前蛋白浓度见COA
生化机理
S100A1: Small calcium binding protein that plays important roles in several biological processes such as Ca2+ homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca2+ levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca2+ cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca2+-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca2+ dissociation and myofilament mechanics in order to improve relaxation during diastole. S100B: Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain. Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (By similarity). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (By similarity).
Post-translational: S100A1: Glutathionylated; glutathionylation increases affinity to calcium about 10-fold.
种属
牛(Bovine)
来源
天然
预测分子量
6 kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 50 mM Tris-HCl, 50 mM NaCl, 100 μM DTT, pH 7.5.
浓度
冻干前蛋白浓度见COA
复溶
离心后，建议加入去离子水补至原体积（详见 COA 证书），随后轻柔混匀，保证充分均一。如需进一步稀释，请使用冻干缓冲液。
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（大于1年）；复溶后建议分装，避免反复冻融。
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
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