计算溶液所需的质量、体积或浓度。
免疫球蛋白M, μ链 来源于正常人血浆
有货
为什么选择此级别
冻干粉,天然 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
经定向二硫键还原去除轻链后的 IgM 重链。μ 链在不同物种间高度保守。
蛋白生物学功能
IgM 重链，即 μ 链，是 IgM 抗体的核心组成部分，由 576 个氨基酸组成。每个 IgM 分子包含五条 μ 链，形成五聚体结构，可实现强效抗原结合并高效激活补体系统。μ 链具有很高的跨物种进化保守性，保守程度高于 IgG 或 IgA 重链，凸显其在免疫应答中的关键作用。μ 链的纯化通常通过还原其与 κ、λ 轻链相连的二硫键实现，可用于开展详尽的结构与功能研究。
常见研究用途
临床层面，μ 链异常与免疫缺陷病（如选择性 IgM 缺乏症）以及部分 B 细胞恶性肿瘤（如华氏巨球蛋白血症）相关。此外，μ 链可作为早期感染诊断检测的靶标，也是 B 细胞发育、免疫系统进化研究中的重要标志物。
常见应用：抗血清制备、免疫系统研究。
The heavy chain of IgM lacking the light chain due to directed disulfide bond reduction. The μ chain is highly conserved across species.
Role of protein in biology
The IgM heavy chain, known as the mu (μ) chain, is a fundamental component of IgM antibodies and consists of 576 amino acids. Each IgM molecule is composed of five mu chains, forming a pentameric structure that enables robust antigen binding and efficient activation of the complement system. The mu chain is distinguished by its high degree of evolutionary conservation across species, more so than the heavy chains of IgG or IgA, underscoring its essential role in the immune response. Purification of the mu chain is typically achieved by reducing the disulfide bonds that link it to the kappa and lambda light chains, allowing for detailed structural and functional studies.
Common research uses
Clinically, abnormalities in the mu chain can be associated with immunodeficiency disorders, such as selective IgM deficiency, and certain B-cell malignancies like Waldenström’s macroglobulinemia. Additionally, the mu chain serves as a target in diagnostic assays for early infection and is a key marker in the study of B-cell development and immune system evolution.
Common uses include antisera production, immune system research.
规格
产品名称
免疫球蛋白M, μ链 来源于正常人血浆
规格或纯度
天然, 冻干粉, ≥95%(SDS-PAGE), 冻干前蛋白浓度见COA
生化机理
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen. Isoform 1: Constant region of secreted IgM (sIgM), also known as the Fc region of IgM antibody. Able to multimerize, forms high order polymers, mainly pentamers and occasionally hexamers, providing for multivalency and high avidity recognition of antigens. Natural sIgM are polyreactive and recognize conserved self- and pathogen-derived structures, whereas immune sIgM are secreted only upon exposure to pathogens and are antigen-specific. Both natural and immune sIgM are required for an efficient humoral immune response to infection (By similarity). Mediates sIgM effector functions mostly via Fc receptors and the complement system. On lymphoid cells binds high-affinity Fc receptor FCMR and promotes induction of an efficient neutralizing IgG response while maintaining tolerance to self-antigens. Recruits C1q complement component to initiate the classical complement pathway, facilitating the recognition and neutralization of pathogens by the host. Together with C1q and mannose-binding lectin promotes the phagocytosis of apoptotic cells by macrophages, ensuring the clearance of potential autoimmune epitopes from tissues. Involved in mucosal immunity. It is transported by transcytosis across mucosal epithelium by PIGR and secreted on the apical side in complex with PIGR secretory component to scan mucosal lining for pathogens. IgM-antigen complexes undergo FCMR-mediated retrotranscytosis across mucosal M cells toward antigen-presenting cells in mucosal lymphoid tissues. Isoform 2: Constant region of membrane-bound IgM, part of the B cell receptor complex (BCR). IgM BCR provides constitutive tonic signaling for B cell survival. Mediates pre-BCR signaling that regulates B cell selection and rearrangement of Ig genes via allelic exclusion.
Post-translational: N-glycosylated; important for IgM secretion and its localization at the plasma membrane. The interaction with FCMR is glycan-independent.
种属
人(Human)
来源
天然
预测分子量
75 kDa
分子类型
蛋白质
产品线
biological_products
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 1.0 M acetic acid.
浓度
冻干前蛋白浓度见COA
复溶
离心后，加入 1.0 M 乙酸至接近原始体积（详见 COA），随后缓慢旋摇混匀，保证充分均一。 所有稀释操作均须使用乙酸溶液。
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（>1年）；复溶后建议分装，避免反复冻融。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
常见问题
本产品的纯度是多少？如何测定？
本产品的纯度为 ≥95%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）