计算溶液所需的质量、体积或浓度。
免疫球蛋白G1 (IgG1), Fab片段 来源于人骨髓瘤血浆
有货
为什么选择此级别
天然 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
经蛋白水解酶消化得到的 IgG 单价抗原结合片段。IgG Fab 保留抗原特异性可变区，不含可激活免疫的 Fc 段。
蛋白生物学功能
IgG Fab（抗原结合片段）是免疫球蛋白 G 经蛋白酶水解产生的单价片段。每个 Fab 片段保留负责抗原特异性的可变区（VH 和 VL），但缺失 Fc 段介导的效应功能，可实现靶向抗原中和，且不会触发免疫激活。该结构特点可避免交联引发的沉淀，或是细胞的 “成斑 / 成帽” 效应，因此 Fab 片段非常适合在溶液体系或细胞表面精准靶向抗原。
常见科研用途
诊断领域中，Fab 片段能够避免 Fc 段带来的交叉反应，从而实现多重免疫检测，可在流式细胞术与免疫组化实验中同时检测多种抗原。其单价特性可在高特异性实验中最大限度降低背景干扰。新兴应用方向包括自身免疫病干预：Fab 片段可中和自身抗体，同时规避补体激活。IgG Fab 兼具靶向结合能力与较低免疫原性，是临床与科研领域用途广泛的工具。
常见应用：治疗药物研发、免疫系统研究、流式细胞术检测、免疫组化检测。
The monovalent antigen binding fragment of IgG generated by proteolytic digestion. IgG Fab retains the antigen specific variable domains, while lacking the immune activating Fc regions.
Role of protein in biology
The IgG Fab (Fragment antigen-binding) fragment is a monovalent proteolytic product of Immunoglobulin G. Each Fab fragment retains the variable domains (VH and VL) responsible for antigen specificity while lacking the Fc-mediated effector functions, enabling targeted antigen neutralization without immune activation. This structural configuration prevents cross-linking-induced precipitation or cellular "patching/capping," making Fab fragments ideal for precise antigen targeting in solution or on cell surfaces.
Common research uses
Diagnostically, Fab fragments enable multiplex immunoassays by preventing Fc-mediated cross-reactivity, facilitating simultaneous detection of multiple antigens in flow cytometry and immunohistochemistry. Their monovalent nature ensures minimal background noise in assays requiring high specificity. Emerging applications include autoimmune disease management, where Fab fragments neutralize autoantibodies while circumventing complement activation. By combining targeted binding with reduced immunogenicity, IgG Fab fragments represent a versatile tool in both clinical and research settings.
Common uses include therapeutics research, immune system research, flow cytometry assays, immunohistochemistry assays.
规格
产品名称
免疫球蛋白G1 (IgG1), Fab片段 来源于人骨髓瘤血浆
规格或纯度
天然, ≥95%(SDS-PAGE), 蛋白浓度见COA
生化机理
Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure. The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE.The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.
Post-translational: N-glycosylated. Carries predominantly biantennary complex-type glycans attached at Asn-180 residue on the Fc region of each heavy chain. Unique Fc glycan profiles found in secreted IgGs are induced in an antigen-specific way, likely programmed during B cell priming to mount an appropriate Ig effector response. The core glycan is composed of two sequential N-acetylglucosamine (GlcNAc) moieties followed by a central mannose (Man) from which two additional Man residues branch out (alpha1,3 and alpha1,6 antennae) each capped with a GlcNAc. Additional sugar molecules can be added to generate over 30 possible glycans. Such sugar modifications include the addition of one fucose at the initial GlcNAc, galactose (Gal) and sialic acid (Neu5Ac) residues at antennary GlcNAc or a bisecting GlcNAc to the core Man. Variable addition of sugars account for different IgG functional states associated with antibody-dependent cellular cytotoxicity or phagocytosis and inflammatory responses such as complement activation and cytokine secretion. Fc N-glycan diversity is further enhanced by asymmetric glycan pairing on the heavy chains. Fc N-glycan sialylation is linked to anti-inflammatory effects. It regulates Fc effector functions through conformational changes leading to preferential interaction with type II Fc receptors while reducing binding to type I Fc receptors. During plasmablast response, sialylated Fc domains within immune complexes signal via FCER2/CD23 and drive the selection of B cells with high affinity for antigen. Fc Igs carrying afucosylated N-glycans preferentially activate FCGR3A, antigen-dependent cellular cytotoxicity and antitumor immunity. (Microbial infection) Deglycosylation on Asn-180 by S. pyogenes EndoS or Endos2 endoglucosidases prevents interaction between immunoglobulin-gamma (IgG) and Fc receptors, impairing ability to activate the complement pathway.
种属
人(Human)
来源
天然
预测分子量
50 kDa
分子类型
蛋白质
产品线
biological_products
储存与运输
物理形态
液体
储存缓冲液
20 mM Phosphate, 150 mM NaCl, 0.05% NaN₃, pH 7.4.
防腐剂
0.05% Sodium azide
浓度
蛋白浓度见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（>1年）；收货后建议分装，避免反复冻融。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
常见问题
本产品的纯度是多少？如何测定？
本产品的纯度为 ≥95%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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