Complement C5 is part of the terminal sequence in the complement pathway. In particular, it is cleaved into C5a and C5b which are responsible for chemotaxis, inflammation, and initiating the formation of the membrane attack complex (MAC). Drugs that inhibit complement C5 have been used in Paroxysmal nocturnal hemoglobinuria (PNH) patients to lessen thrombotic complications by lessening intravascular hemolysis.
Complement component C5 is processed by convertase enzymes in a cascade modulated in a unique way. The multi-subunit catalytic complex initially shows little activity against C5, but instead cleaves C3. A C3 cleavage product C3b covalently attaches to the complex and shifts its specificity to C5 by a factor of 1000.
Purity: >=95% by SDS-PAGE.
Reacts to C5 antisera on Western Blot
Not tested for biological activity.
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways.
Post-translational: C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. During activation of the complement systems, the alpha chain is cleaved into C5a and C5b by the C5 convertase: C5b stays linked to the beta chain, while C5a is released in the plasma. The alpha chain is cleaved by the serine protease complement C2b component of the C5 convertase to generate C5a and C5b following activation by the classical, lectin and GZMK complement systems. The alpha chain is cleaved by CFB component of the C5 convertase to generate C5a and C5b following activation by the alternative complement system.
生物活性
Titer >100,000 C5H50 U/mg; >60% of C5 activity in NHS on a mg/mg basis.
种属
人(Human)
来源
天然
预测分子量
190 kDa (2 chains)
CAS编号和信息
80295-53-0
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
10 mM Sodium phosphate, 145 mM NaCl, pH 7.2.
浓度
蛋白浓度: 0.95-1.2 mg/ml
储存条件
-80℃储存,避免反复冻融
运输条件
特低温运输
稳定性与储存
长期储存-80℃（3年）；收货后建议分装，避免反复冻融。
单位定义
One C5H50 unit measured by classical pathway activation is defined as the amount of C5 required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C5-Dpl serum for 30 minutes at 37°C in a total reaction volume of
ZJ26F1040053
危险品化学品经营许可证（带存储）