Butyrylcholinesterase (BChE) is a tetrameric glycoprotein belonging to the family of serine hydrolases known as cholinesterases. While there is currently no know physiological function for BChE, it has been shown to behave as a bioscavenger against cocaine toxicity and organophosphorus nerve agents.
Prepared from plasma shown to be non reactive for HbsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
ActiBioPure™, 生物活性, 高性能, EnzymoPure™, ≥90%(SDS-PAGE), Pre-lyophilization Protein Concentration
生化机理
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
Post-translational: N-glycosylated. No other PTM detected. The major N-glycan structures are of the complex diantennary type with 1 and 2 N-acetylneuraminic acid molecules (Neu5Ac) making up approximately 33% and 47% of the total N-glycans, respectively. Only low amounts of fucosylated diantennary N-glycans are detected (approximately 2%). Triantennary N-glycans with or without fucose amount to approximately 13%, whereas 5% of the total N-glycans are of the oligomannosidic or hybrid type.
生物活性
≥50 U/mg protein
种属
人(Human)
来源
天然
预测分子量
340 kDa
CAS编号和信息
9001-08-5
酶学委员会编号
3.1.1.8
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 100 mM L-Histidine, 240 mM sucrose, 0.3 mM TWEEN 20, pH 6.0
浓度
Pre-lyophilization Protein Concentration
复溶
After centrifugation, we suggest addition of de-ionized H2O to the original volume (See COA), followed by gentle mixing to ensure adequate homogenization.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃(大于1年);复溶后建议分装,避免反复冻融。
单位定义
One unit will hydrolyze one micromole of butyrylcholine to choline and butyrate per minute at pH 8.0 at 37℃.