计算溶液所需的质量、体积或浓度。
载脂蛋白AI 来源于人血浆
有货
为什么选择此级别
低内毒素,天然 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-80℃储存,避免反复冻融。特低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
载脂蛋白AI 是高密度脂蛋白（HDL）的主要蛋白组分，是卵磷脂‑胆固醇酰基转移酶的激活剂，可催化胆固醇发生酯化反应。载脂蛋白 A‑I 长期作为心血管疾病的生物标志物。
蛋白生物学作用
载脂蛋白AI（Apo AI）占高密度脂蛋白相关载脂蛋白总量的 75%，是胆固醇逆向转运（RCT）的关键分子，介导胆固醇从外周组织向肝脏外流。作为 HDL 主要结构组分，Apo AI 可稳定盘状 HDL 颗粒，激活卵磷脂‑胆固醇酰基转移酶（LCAT）使胆固醇酯化，促进 HDL 成熟。该蛋白具备抗炎活性，可阻断 T 细胞‑单核细胞相互作用，降低 TNF‑α、IL‑1β 生成，并中和氧化型低密度脂蛋白。人体正常血浆水平为 90‑130 mg/100 mL，其浓度与冠心病（CHD）风险呈负相关。
常见研究用途
Apo‑AI 缺陷会引发丹吉尔病，该病特征为 HDL 几乎缺失、巨噬细胞胆固醇蓄积以及早发性动脉粥样硬化。APOA1 p.Leu202Arg等突变可诱发系统性淀粉样变性，造成心、肾功能损伤。Apo‑AI 水平降低还与胰岛素抵抗、β 细胞功能受损以及糖尿病进展相关。与之相反，Apo‑AI 过表达可增强巨噬细胞特异性胆固醇逆向转运，减轻动脉粥样硬化。
常见应用：体外诊断标准品、校准品与质控品、脂质代谢研究、生物治疗研究、细胞水平实验。
Apolipoprotein AI is the primary protein component of HDL, and activator of lecithin-cholesterol acyltransferase for the esterification of cholesterol. Apo AI has a long history as a cardiovascular disease biomarker.
Role of protein in biology
Apolipoprotein AI (Apo AI), constituting 75% of HDL-associated apolipoproteins, is a critical for reverse cholesterol transport (RCT), mediating cholesterol efflux from peripheral tissues to the liver. As the primary structural component of HDL, Apo AI stabilizes discoidal HDL particles, activates lecithin-cholesterol acyltransferase (LCAT) to esterify cholesterol, and facilitates HDL maturatioN. Its anti-inflammatory properties include blocking T-cell–monocyte interactions, reducing TNF-α and IL-1β production, and neutralizing oxidized LDL. Normal plasma levels range from 90–130 mg/100 mL, with concentrations inversely correlating with coronary heart disease (CHD) risk.
Common research uses
Deficiencies in Apo AI underlie Tangier disease, characterized by near-absent HDL, cholesterol-laden macrophages, and premature atherosclerosis. Mutations like APOA1 p.Leu202Arg cause systemic amyloidosis, leading to cardiac and renal dysfunction. Reduced Apo AI levels also associate with insulin resistance, impaired β-cell function, and diabetes progression. Conversely, Apo AI overexpression enhances macrophage-specific RCT, attenuating atherosclerosis.
Common uses include IVD standards, calibrators, and controls, lipid metabolism studies, biotherapeutic studies, cell based assays.
规格
产品名称
载脂蛋白AI 来源于人血浆
规格或纯度
天然, 低内毒素, ≥95%(SDS-PAGE), 蛋白浓度见COA
生化机理
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Post-translational: Glycosylated. Palmitoylated. Phosphorylation sites are present in the extracellular medium. Some tyrosine residues are chlorinated and nitrated: chlorination and nitration takes place when the lipid-free form of APOA1 is exposed to hypochlorous acid and peroxynitrite oxidants, respectively, which are generated by myeloperoxidase (MPO). Chlorinated and nitrated at Tyr-53, Tyr-190, Tyr-216 and Tyr-260; Tyr-216 constitutes the major site of chlorination and nitration. According to a report, chlorination of Tyr-216 inactivates APOA1 and impairs cholesterol transport. According to another report, however, other sites, such as tryptophan residues, can be modified by oxidants generated by MPO in absence of tyrosine residues.
种属
人(Human)
来源
天然
预测分子量
28 kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
10 mM NH₄HCO₃, pH 7.4.
浓度
蛋白浓度见COA
储存条件
-80℃储存,避免反复冻融
运输条件
特低温运输
稳定性与储存
长期储存-80℃（大于1年）；收货后建议分装，避免反复冻融。
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