Angiotensinogen (AGT) is a glycoprotein found in plasma at a concentration of 2.8-7.1 mg per 100mL in healthy individuals. AGT is produced primarily by the liver but also is synthesized in the brain, large arteries, kidneys and adipose tissue. In plasma it exists as a monomeric form (55-65 kDa) as well as a high molecular mass form (200-550 kDa). In non-pregnant individuals with normal blood pressure, the high molecular mass form accounts for 3% of total circulating AGT. In the first half of pregnancy the total AGT increases by 4-fold and the high molecular mass form increases by 20-fold. Up to 80% of the total AGT in amniotic fluid is the high molecular mass form. Angiotensinogen is an essential component of the renin-angiotensin system, a potent regulator of blood pressure, body fluid and electrolyte homeostasis. It functions as the substrate for human renin and is the precursor molecule for Angiotensins 1,2,3,4, 1-9, 1-7, 1-5 and 1-4. AGT is also a member of the SERPIN family but it is not known to possess protease-inhibitory activity. Levels in plasma are increased by plasma corticosteroid, estrogen, thyroid hormone and angiotensin-2 levels. Extinction Coefficient: 0.92 Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
BioReagent, 天然, PBS Only, ≥95%(SDS-PAGE), Pre-lyophilization Protein Concentration
生化机理
Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor (By similarity). Angiotensin-3: Stimulates aldosterone release. Angiotensin 1-7: Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity).
Post-translational modification:Beta-decarboxylation of Asp-25 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor (PubMed:17138938).1 PublicationIn response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin.
种属
人(Human)
来源
天然
预测分子量
55-65 kDa
CAS编号和信息
11002-13-4
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 30 mM Sodium phosphate, pH 6.8.
浓度
Pre-lyophilization Protein Concentration
复溶
After centrifugation, we suggest addition of de-ionized H2O to the original volume (See COA), followed by gentle mixingto ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
长期储存-20℃（>1年）；复溶后建议分装，避免反复冻融。
图片
Angiotensinogen from Human Plasma (np001156) - SDS-PAGE
2 μg/lane of Angiotensinogen from Human Plasma was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 55 - 65 kDa.
ZJ25F1129646
危险品化学品经营许可证（带存储）