无叠氮钠,高性能 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
背景
链霉亲和素是一种来自streptomyces avidinii菌的非糖基化蛋白。链亲和素同源四聚体对生物素具有特别高的非共价结合亲和力。当与酶（如辣根过氧化物酶或碱性磷酸酶）形成偶联物并与比色或发光底物发育系统结合时，链霉亲和素与生物素化抗体在许多应用中得到广泛应用，包括Western blot、ELISA、ELISPOT、免疫细胞化学和免疫组化。
推荐工作浓度
WB : 1:10000-1:100000
ELISA: 1:1000-1:10000
避免使用含有生物素的稀释液，试剂不得含有叠氮化钠，叠氮化钠会抑制辣根过氧化物酶的活性。实际试验操作过程中可根据具体实验情况适当调整Streptavidin(HRP)的稀释比例。
Background
Streptavidin is a non-glycosylated protein from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.
Recommended Usage：
WB: 1:10000 -1:100000
ELISA: 1:1000-1:10000
Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.
WB-Streptavidin (HRP) (np156148)
Various whole cell lysates were subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:10000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 10s.
ELISA-Streptavidin (HRP) (np156148)
ELISA results of Streptavidin (HRP) (np156148) tested against Biotin-BSA. Each well was coated in duplicate with 1 ng of Biotin-BSA.
WB-Streptavidin (HRP) (np156148)
HT-29 whole cell lysate was subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:5000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 8s.
1/1000-1/10000
1:10000-1:100000
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0737773 |
分析证书 | np156148 |
| ZJ26F0737772 |
分析证书 | np156148 |
| ZJ23F0300087 |
分析证书 | np156148 |
| ZJ25F0723953 |
分析证书 | np156148 |
| ZJ26F0333146 |
分析证书 | np156148 |
| ZJ26F0333147 |
分析证书 | np156148 |
| ZJ25F0723954 |
分析证书 | np156148 |
| ZJ24F0708371 |
分析证书 | np156148 |
| ZJ24F0708372 |
分析证书 | np156148 |
| ZJ25F0319100 |
分析证书 | np156148 |
| ZJ25F0319099 |
分析证书 | np156148 |
| ZJ23F0300088 |
分析证书 | np156148 |
危险品化学品经营许可证（带存储）