Prepared from fresh frozen human plasma. Human Prothrombin is a glycoprotein of molecular weight 72,000, and consists of a single polypeptide chain. Activation of Prothrombin by Factor Va, Xa and phospholipids yields the serine protease Thrombin. The human Prothrombin is >95% via SDS-PAGE and shows no reduction upon incubation with 2- mercaptoethanol.
Extinction Coefficient (1%) = 13.6
The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.
规格
产品名称
凝血酶原
别名
凝血因子 Ⅱ | 凝血第二因子
英文别名
Prothrombin from Human plasma | Human Prothrombin | Human Prothrombin (Factor II) Native Protein | Prothrombin, Human Plasma | Coagulation factor II | F2 | PT | THPH1 | RPRGL2
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces. Activity regulation: Activity is promoted in the presence of negatively charged surfaces, such as polyphosphate and dextran sulfate. Inhibited by SERPINA5.
Post-translational: The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin. N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). In the penultimate step of the coagulation cascade, prothrombin is converted to thrombin by the prothrombinase complex composed of factor Xa (F10), cofactor Va (F5), and phospholipids. This activation requires factor Xa-catalyzed sequential cleavage at 2 sites, Arg-314 and Arg-363, along 2 possible pathways. In the first pathway, the first cleavage occurs at Arg-314, leading to the formation of the inactive intermediate prethrombin-2. This pathway preferentially occurs on platelets and in the absence of cofactor Va. In the second pathway, the first cleavage occurs at Arg-363, which separates protease domain into 2 chains that remain connected through a disulfide bond and generates the active intermediate meizothrombin. The presence of cofactor Va directs activation along the meizothrombin pathway and greatly accelerates the rate of cleavage at Arg-363, but has a smaller effect on the cleavage of meizothrombin at Arg-314. Meizothrombin accumulates as an intermediate when prothrombinase is assembled on the membrane of red blood cells.
种属
人(Human)
预测分子量
72 kDa
CAS编号和信息
9001-26-7
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris-HCl, 0.1M NaCl, pH 7.4.
浓度
来源于人血浆; 冻干前浓度：见COA
复溶
We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.
ZJ26F0737102
危险品化学品经营许可证（带存储）