Prepared from fresh frozen human plasma. Human Factor VII is a single-chain vitamin K dependent glycoprotein found in trace quantities in plasma (0.5 mg/Liter). In the Tissue Factor Pathway of coagulation, Human Factor Vlla, in the presence of calcium ions and tissue factor, activates Factors IX and X to their enzymatically active forms, Factor IXa and Xa.
The Human Factor VII is >95% on SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.
Extinction Coefficient (1%) = 13.9
The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.
规格
产品名称
凝血因子VII
别名
凝血因子Ⅶ | 血清凝血酶原转化加速因子 | 前转化素
英文别名
Human Factor VII | Human Factor VII Native Protein | Proconvertin | Coagulation factor VII | F7 | SPCA
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa also converts factor IX to factor IXa in the presence of tissue factor and calcium.
Post-translational: The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Can be either O-glucosylated or O-xylosylated at Ser-112 by POGLUT1 in vitro.
生物活性
见 COA
种属
人(Human)
来源
天然
预测分子量
50 kDa
CAS编号和信息
9001-25-6
酶学委员会编号
EC 3.4.21.21
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 0.03M Tris-HCl, 0.15M NaCl, 0.1% PEG 8000, 4% Ficoll Type 400, 0.5mM Benzamidine, pH 7.4.
浓度
来源于人血浆; 冻干前浓度：见COA
复溶
We suggest hydrating the protein with a good quality water to the original volume. After hydration aliquot into a useful (one time use) size and freeze at <-60℃.
ZJ26F0736969
危险品化学品经营许可证（带存储）