Purified from fresh frozen human plasma using a combination of salt precipitations and column chromatography. The protein purity is determined by SDS-PAGE and shows total reduction upon incubation with 2-mercaptoethanol. Protein C is activated to the serine protease, Activated Protein C (APC), by a-thrombin or the complex of a-thrombin/thrombomodulin and is a potent anticoagulant through the selective inactivation of Factors Va and VIIIa.
Buffer composition = 20 mM Tris-HCl/0.1 M NaCl/1 mM Benzamidine/pH 7.4
Extinction Coefficient (1%) = 14.5
Molecular Weight = 62,000 daltons
The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.
规格
产品名称
蛋白C (PC)
别名
蛋白 C 酶原 | 维生素 K 依赖蛋白 C
英文别名
Human protein C | Protein C from human plasma | Human Protein C Native Protein | Vitamin K-dependent protein C | inactive Protein C | z-PC | zymogen Protein C | protein C, inactivator of coagulation factors Va and VIIIa | PROC | PC | APC | PROC1 | THPH3 |
Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function. Miscellaneous: Calcium also binds, with stronger affinity to another site, beyond the GLA domain. This GLA-independent binding site is necessary for the recognition of the thrombin-thrombomodulin complex.
Post-translational: Carboxylated by vitamin K-dependent GGCX to form gamma-carboxyglutamate; these residues are essential for the binding of calcium. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
种属
人(Human)
来源
天然
预测分子量
62 kDa
CAS编号和信息
925910-74-3
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20 mM Tris-HCl, 0.1 M NaCl, 1mM Benzamidine, pH 7.4
浓度
来源于人血浆; 冻干前浓度：见COA
复溶
We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.
ZJ26F0738062
危险品化学品经营许可证（带存储）