Purified from fresh frozen human plasma. Human Factor XII is part of the contact activation system. Human Factor XII is a single chain glycoprotein with a molecular weight of about 80,000. Once activated, principally from the action of kallikrein, Factor XII is converted into an active serine protease (Factor α-Xlla) that functions in the in vivo initiation of blood coagulation, fibrinolysis, and kinin formation. Human Factor XII has been purified by ion exchange chromatography. Protein purity is determined by SDS-PAGE and activity is determined via clotting assay. The Human Factor XII is >95% as judged by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol.
Extinction Coefficient (1%) =14.1
The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.
规格
产品名称
凝血因子XII
英文别名
Human Factor XII | Coagulation Factor XII, Human Plasma | Human Factor XII Native Protein | Hageman Factor | Stuart factor | F12 | HAF | HAE3 | HAEX
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. Catalytic activity: Selective cleavage of Arg-|-Ile bonds in factor VII to form factor VIIa and factor XI to form factor XIa.
Post-translational: Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2. O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
生物活性
见COA
种属
人(Human)
来源
天然
预测分子量
80 kDa
CAS编号和信息
9001-30-3
酶学委员会编号
EC 3.4.21.38
分子类型
酶
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 4mM Sodium acetate-HCl, 0.15M NaCl, pH 5.3.
浓度
来源于人血浆; 冻干前浓度：见COA
复溶
We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.
ZJ26F0737110
危险品化学品经营许可证（带存储）