纤连蛋白 (FN)

Name: 纤连蛋白 (FN)
SKU: np330969
Availability: InStock

CAS: 86088-83-7 货号: np330969 EC号: 289-149-2

1篇引用文献 COA

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级别和纯度: 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? 无菌 ? ≥90%(SDS-PAGE) 来源于人血浆

Accession #

P02751

生物活性

Human Fibronectin has been tested for its ability to promote attachment and spreading using BHK-21 cells.

规格

库存

价格

数量

50μg

np330969-50μg

期货

¥599.90

250μg

np330969-250μg

期货

¥1,599.90

1mg

np330969-1mg

期货

¥3,199.90

5mg

np330969-5mg

期货

¥7,999.90

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为什么选择此级别

ActiBioPure™,天然,无菌,生物活性,高性能级提供 ≥90%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

2-8°C储存。低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。

概述

Fibronectin is a broad range natural cell adhesion factor. It is a 440-500 kDa dimeric glycoprotein consisting of two similar 220-250 kDa subunits linked by two disulfide bonds. It is found as a dimer in plasma and in multimeric form in the extracellular matrix and on cell surfaces. Its primary function is related to cell adhesion to the extracellular matrix which occurs via the Arg-Gly-Asp-Ser (RGDS) sequence of fibronectin with the appropriate transmembrane integrin receptor of the cells. Other domains of fibronectin are also involved with this adhesion process and may involve interaction with collagen, heparin and other cell surface glycosaminoglycans. The conformation and orientation of adsorbed fibronectin is also important and has an effect on cell spreading and strength of adhesion of endothelial cells.

This product is used as a thin coating on tissue-culture surfaces to promote attachment, spreading, and proliferation of a variety of cell types. The principal functions of fibronectin appear to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis.

Fibronectin addition to serum free medium promotes cell adhesion. More significant effects are observed with BHK, CHO and other cell lines by coating of cultureware with fibronectin at 1-5 ug/cm².

Quality

Purity ≥90% (SDS-PAGE)
Tested for ability to promote attachment and spreading of BHK-21 cells
Found negative for bacteria, fungi, and mycoplasma

Source: Human plasma.

NOTE: The source plasma was tested and found nonreactive for hepatitis B surface antigen (HBsAg) and negative for antibodies to human immunodeficiency virus (HIV), hepatitis C virus (anti-HCV), and syphilis (RPR). Nevertheless, this product should be handled using the same safety precautions used when handling potentially infectious material.

RECONSTITUTION & USE:

TO ENSURE PROPER SOLUBILITY OF HFN THIS RECONSTITUTION PROCEDURE MUST BE FOLLOWED.

Equilibrate vial to room temperature. Resuspend in sterile distilled water to a concentration of 1mg/ml. Allow 30 minutes for material to go into solution. DO NOT AGITATE OR SWIRL. If entire amount of material is not to be used immediately, transfer into appropriate aliquots and store at −20°C. It is recommended that solubilized product is used within two weeks. DO NOT STORE IN FROST-FREE FREEZER. AVOID MULTIPLE FREEZE THAWS.

Human Fibronectin is generally used in the concentration range of 1-5 micrograms per cm² of growth surface for attachment or at 5 micrograms per ml as a media additive.

Coating Procedure

Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

Dilute fibronectin to desired concentration using serum-free culture Ca²⁺, Mg²⁺ free medium or buffer at pH 7-9. The final solution should be sufficiently dilute so that the volume added will cover the surface evenly. Example: If the final coating concentration will be 5 µg/cm², dilute the material to 50 µg/ml and add 1 ml/35 mm dish, 3 ml/60 mm dish, etc. NOTE: Because of the CAPS component in the HFN preparation, buffers of media containing Ca²⁺ and/or Mg²⁺ added to the HFN may result in the formation of insoluble metal hydroxides. This will not occur if the buffering capacity of the diluent brings the pH to 8.0 or lower.
Add appropriate amount of diluted fibronectin to culture surface.
Incubate at room temperature for 1 hour.
Aspirate remaining material.
Rinse plates carefully with dH₂O - avoid scraping bottom surface.
Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

规格

产品名称

纤连蛋白 (FN)

别名

纤维连接蛋白 | 人纤连蛋白

英文别名

规格或纯度

生物活性, ActiBioPure™, 天然, 高性能, 无菌, ≥90%(SDS-PAGE), 来源于人血浆

生化机理

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Anastellin: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. Features: Showing features for site, dna binding. Post-translational: Sulfated. It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).By SimilarityPhosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin. Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.By SimilaritySerotonylated on Gln residues by TGM2 in response to hypoxia.

生物活性

Human Fibronectin has been tested for its ability to promote attachment and spreading using BHK-21 cells.

种属

人(Human)

来源

天然

预测分子量

440 kDa

CAS编号和信息

86088-83-7

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from 100 mM CAPS, 0.15 M NaCl, 1 mM Calcium chloride, pH 11.0.

浓度

来源于人血浆

复溶

Equilibrate vial to room temperature. Resuspend in sterile distilled water to a concentration of 1mg/ml. Allow 30 minutes for material to go into solution. DO NOT AGITATE OR SWIRL. If entire amount of material is not to be used immediately, transfer into appropriate aliquots and store at -20°C. It is recommended that solubilized product is used within two weeks. DO NOT STORE IN FROST-FREE FREEZER. AVOID MULTIPLE FREEZE THAWS.

储存条件

2-8°C储存

运输条件

低温运输

稳定性与储存

长期储存2-8℃，请勿冷冻。复溶后建议分装，避免反复冻融。

技术文档

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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技术文档和文章

细胞培养包被体系中玻连蛋白、层粘连蛋白与纤连蛋白的适用性比较

目录：技术文章

探索主题:

细胞培养细胞外基质包被材料

Applicability Comparison of Vitronectin, Laminin and Fibronectin in Cell Culture Coating Systems

目录：技术文章

探索主题:

cell culture Extracellular matrix coating material

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纤连蛋白 (FN)

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