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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
氧基抗氧化能力(Oxygen Radical Absorbance Capacity, ORAC)是一种检测各种样品中生物分子抗氧化能力的经典方法。ORAC 方法对抗氧化性能的评价具有特异性好、灵敏度高、测定范围广,以及适合抗氧化活性的高通量筛选等优点,在天然产物抗氧化提取物中得到越来越多的应用,食品及功能食品企业普遍采用ORAC 作为功能食品的重要评价标准。 本试剂盒检测原理是以荧光素钠为荧光探针,偶氮类化合物 AAPH 作为过氧自由基来源,根据自由基破坏荧光探针,使荧光强度产生变化,荧光强度的变化大小反映自由基破坏的程度。在抗氧化剂存在时,它可以抑制由自由基引起的荧光变化,抑制程度反映了它对自由基的抗氧化能力。
Oxygen Radical Absorbance Capacity (ORAC) is a classic method for detecting the antioxidant capacity of biomolecules in various samples. The ORAC method offers distinct advantages for evaluating antioxidant performance, including high specificity, excellent sensitivity, a broad detection range, and suitability for high-throughput screening of antioxidant activity. Consequently, it is increasingly applied in the assessment of natural product antioxidant extracts and is widely adopted by the food and functional food industries as a key evaluation standard for functional foods. The detection principle of this kit is as follows: Sodium fluorescein is used as the fluorescent probe, and the azo compound AAPH serves as the source of peroxyl radicals. The radicals degrade the fluorescent probe, causing a change in fluorescence intensity. The extent of this change reflects the degree of radical-induced damage. In the presence of antioxidants, they can inhibit the fluorescence change caused by the radicals. The degree of inhibition reflects their antioxidant capacity against the radicals.