MEM (Minimum Essential Medium), also known as minimal essential medium, is a commonly used cell culture medium. MEM contains only 12 essential amino acids, glutamine, and 8 vitamins, with a simple composition. It is primarily used for adherent cell culture, and can also be adapted for other cell types after modification of the formulation.
Opti‑MEM medium is a modified version of Minimal Essential Medium (MEM), supplemented with transferrin, insulin, hypoxanthine, thymidine, and other trace elements. It allows for a 50% reduction in fetal bovine serum (FBS) supplementation while maintaining the same growth rate and morphology. Opti‑MEM can be used for various suspension and adherent mammalian cells, including Sp2, AE‑1, CHO, BHK‑21, HEK, and primary fibroblasts.
This product utilizes a sodium bicarbonate buffering system (2.4 g/L) and therefore requires a 5–10% CO₂ atmosphere to maintain physiological pH.
Precautions
1. This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination.
2. To maintain optimal performance, avoid freeze‑thaw cycles.
3. This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.
危险品化学品经营许可证（带存储）