计算溶液所需的质量、体积或浓度。
碘化丙啶染色液 (PI) (P1372285) - Flow Cytometry
Dot plot showing the viability of cryopreserved C57BL/6 Mouse splenocytes stained with P1372285 (Right). Left - Unstained cells.
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
碘化丙啶(PI)染色液常用于对细胞坏死和细胞凋亡的染色及评估,以及细胞周期分析中DNA含量的确定, 广泛应用于流式细胞分析。
DNA与PI结合后产生的最大发射波长为615-620nm。当被488激光激发时,检测PE的B2 通道或PerCP-Vio® 700、PE-Cy™5 等的B3 通道中可检测到 PI。
注意事项:
PI能够潜在致癌,请穿实验服并戴一次性手套操作,避免接触皮肤和眼睛。
使用说明:
根据PI染色液应用的不同,建议使用前进行滴定以确定最佳工作浓度。
每100μL样本推荐的细胞用量为1x106,每个样本加入2.5μg 染色液,4℃孵育10min后上机检测。
The PI Staining Solution is suitable for the exclusion of dead cells from flow cytometric analysis. Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis.
The fluorescence emission maximum for DNA-bound PI is about 615 - 620 nm. When excited by a 488 nm laser, PI can therefore be detected in the B2 channel commonly used for PE or in the B3 channel commonly used for PerCP-Vio® 700, PE-Cy™5, etc.
Attention:
Propidium Iodide is a potential carcinogen. Avoid contact with skin and eyes. Wear suitable protective clothing, gloves, and eye/face protection.
Instructions for Use:
It is recommended to titrate the PI Staining Solution to obtain optimal results as applications vary. The suggested use of this solution for viability staining is 2.5μg per million cells in 0.1 ml/test, and incubate for 10 minutes at 4 °C before analysis.
碘化丙啶染色液 (PI) (P1372285) - Flow Cytometry
Dot plot showing the viability of cryopreserved C57BL/6 Mouse splenocytes stained with P1372285 (Right). Left - Unstained cells.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | P1372285 |
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