碘化丙啶(PI)染色液常用于对细胞坏死和细胞凋亡的染色及评估,以及细胞周期分析中DNA含量的确定, 广泛应用于流式细胞分析。
DNA与PI结合后产生的最大发射波长为615-620nm。当被488激光激发时,检测PE的B2 通道或PerCP-Vio® 700、PE-Cy™5 等的B3 通道中可检测到 PI。
注意事项:
PI能够潜在致癌。 请穿实验服并戴一次性手套操作,避免接触皮肤和眼睛。
使用说明:
每100μL样本推荐的细胞用量为1x106,每个样本加入10μL PI染色液,4℃孵育10min后上机检测。
The PI Staining Solution is suitable for the exclusion of dead cells from flow cytometric analysis. Propidium iodide (PI) is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis.
The fluorescence emission maximum for DNA-bound PI is about 615 - 620 nm. When excited by a 488 nm laser, PI can therefore be detected in the B2 channel commonly used for PE or in the B3 channel commonly used for PerCP-Vio® 700, PE-Cy™5, etc.
Attention:
Propidium Iodide is a potential carcinogen. Avoid contact with skin and eyes. Wear suitable protective clothing, gloves, and eye/face protection.
Instructions for Use:
The suggested use of this solution for viability staining is 10 µl per million cells in 0.1 ml/test, and incubate for 10 minutes at 4 °C before analysis.