计算溶液所需的质量、体积或浓度。
蛋白裂解液 (2×）
有货
为什么选择此级别
BioReagent,无菌 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
本产品是阿拉丁经过优化配方，重磅推出的蛋白裂解试剂。具有操作简便且裂解效率高等优点，使用时与样本1: 1添加混匀即可，裂解后的蛋白样品可用于BCA、Western Blot和蛋白组学检测等实验。
操作流程：
1.实验准备：实验前先将本产品从-20℃冷冻环境中取出后进行化冻，将裂解液融化混匀后置于冰上。
2.将样本与裂解液按体积比1:1添加混匀，95°C煮10min。
3.离心(3000g、5min)，取上清，进行BCA蛋白浓度测定、Western Blot和蛋白组学检测等实验。
注意事项和免责说明：
1.本产品不含有蛋白酶抑制剂，根据实验需要自行添加，避免反复冻融，建议分装存储。
2.为保证裂解充分，裂解后蛋白浓度应小于2 μg/μL。
3.本试剂要避光保存。
4.为了您的安全和健康，请穿实验服并戴一次性手套操作。
5.本产品仅限专业人员的科学研究使用，不得用于临床诊断或治疗，不得用于食品或药品。
Product Introduction
This product is a high-performance protein lysis reagent launched by Aladdin with an optimized formula. It features simple operation and high lysis efficiency. When in use, mix it with the sample at a 1:1 ratio (volume ratio), and the lysed protein sample can be used in experiments such as BCA assay, Western Blot, and proteomics detection.
Operation Procedure
1.Experimental Preparation: Before the experiment, take the product out of the -20°C frozen environment to thaw. After the lysis buffer is completely melted and mixed evenly, place it on ice.
2.Add the sample and lysis buffer at a 1:1 volume ratio, mix thoroughly, and boil at 95°C for 10 minutes.
3.Centrifuge at 3000×g for 5 minutes, collect the supernatant, and use it for experiments including BCA protein concentration determination, Western Blot, and proteomics detection.
Precautions and Disclaimer
1.This product does not contain protease inhibitors. Add protease inhibitors by yourself according to experimental needs. Avoid repeated freezing and thawing; aliquoting for storage is recommended.
2.To ensure sufficient lysis, the protein concentration after lysis should be less than 2 μg/μL.
3.Store this reagent in the dark.
4.For your safety and health, wear a lab coat and disposable gloves during operation.
5.This product is limited to scientific research use by professional personnel. It shall not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals.
规格
别名
蛋白裂解试剂 | 裂解和提取缓冲液 | RIPA 裂解液 | 动物组织蛋白裂解液 | SDS裂解液
英文别名
RIPA Lysis Buffer | Lysis Buffer | Tissue Total Protein Lysis Buffer | SDS Lysis Buffer
规格或纯度
BioReagent, 无菌, 2×
稳定性与储存
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark.
英文名称
Protein Lysis Buffer
储存条件
避光,-20°C储存,避免反复冻融
运输条件
超低温运输
产品线
biological_products
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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