计算溶液所需的质量、体积或浓度。
磷脂铁苏木素 (FeH)染色液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
磷脂 (Phospholipid)是指含有磷酸的脂类，属于复合脂。磷脂是生物膜的成分，分为甘油磷脂和鞘磷脂两类，磷脂为两性分子，一端为亲水的含氮或磷的头，另一端为疏水 (亲油)的长烃基链。
磷脂铁苏木素 (FeH)染色液是由Elleder发明的，其原理是磷脂可通过三价铁苏木素显示出来，该法比DAH更简便、快速、敏感，其缺点是组织需要用丙酮脱脂后染色效果才好，而且有时细胞核和脂质会被同时染色呈黑蓝色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
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自备材料：
氯仿、丙酮、蒸馏水、二甲苯或环保脱蜡透明液、DPX封片剂
操作步骤(仅供参考)：
1、取两张连续的切片，室温下用FeH对照液浸泡1张切片1h作为对照，4℃条件下用无水丙酮浸泡另1张切片15min。
2、入FeH固定液固定30min，蒸馏水冲洗。
3、配制FeH Stain，按FeH苏木素染色液: FeH Buffer=1: 3的比例混合，即为FeH Stain。
4、入FeH Stain染色7-10min，蒸馏水冲洗。
5、用蒸馏水等量稀释FeH分化液后，切片入稀释后的FeH分化液浸泡数次。
6、自来水冲洗。
7、丙酮脱水，二甲苯或脱蜡透明液透明，DPX封片剂封固。
染色结果：
磷脂: 蓝色
细胞核: 蓝色
注意事项：
1、磷脂容易溶解，所以组织取出后应立即固定，否则难以着色。
2、该染色液对冰冻切片的染色效果较好。
3、染色后的标本务必避光保存，否则容易褪色。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
Phospholipids are lipids containing phosphate groups and belong to complex lipids. As key components of biological membranes, phospholipids are classified into two categories: glycerophospholipids and sphingophospholipids. Phospholipids are amphipathic molecules, with a hydrophilic head containing nitrogen or phosphorus at one end and a hydrophobic (lipophilic) long hydrocarbon chain at the other end.
Phospholipid Iron Hematoxylin (FeH) Staining Solution was invented by Elleder. Its principle is that phospholipids can be visualized using ferric hematoxylin. Compared with the DAH method, this method is simpler, faster and more sensitive. However, it has drawbacks: tissues need to be defatted with acetone to achieve optimal staining results, and sometimes both cell nuclei and lipids are stained simultaneously, showing a black-blue color. Note: This reagent is for research use only and not intended for clinical diagnosis or other purposes.
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Materials to Be Prepared by User
Chloroform, acetone, distilled water, xylene or environment-friendly deparaffinization and clearing solution, DPX mounting medium
Operating Procedures (For Reference Only)
1. Take two consecutive sections. Immerse one section in FeH Control Solution at room temperature for 1 hour as a control; soak the other section in anhydrous acetone at 4℃ for 15 minutes.
2. Fix the sections in FeH Fixative Solution for 30 minutes, then rinse with distilled water.
3. Prepare the FeH Stain by mixing FeH Hematoxylin Stain and FeH Buffer at a ratio of 1:3.
4. Stain the sections in the FeH Stain for 7–10 minutes, followed by rinsing with distilled water.
5. Dilute the FeH Differentiating Solution with an equal volume of distilled water, then immerse the sections in the diluted solution several times.
6. Rinse the sections with tap water.
7. Dehydrate the sections with acetone, clear them with xylene or deparaffinization and clearing solution, and mount the sections with DPX mounting medium.
Staining Results
Phospholipids: Blue
Cell Nuclei: Blue
Precautions
1. Phospholipids are prone to dissolution. Therefore, tissues should be fixed immediately after collection; otherwise, it will be difficult to achieve satisfactory staining.
2. This stain solution yields optimal results with frozen sections.
3. Stained specimens must be stored away from light to prevent fading.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. Use the reagent as soon as possible after opening to avoid affecting the results of subsequent experiments.
规格
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
英文名称
Phospholipid Iron Hematoxylin (FeH) Staining Solution
应用
Cell Staining
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
组分与储存
| P1508444 | Component | 3×100 mL | Storage |
| P1508444A | FeH对照液 | 10 mL | RT. Store in the dark. |
| P1508444B | FeH固定液 | 100 mL | RT. |
| P1508444C | FeH苏木素染色液 | 25 mL | RT. Store in the dark. |
| P1508444D | FeH Buffer | 75 mL | RT. |
| P1508444E | FeH分化液 | 100 mL | RT. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332878 |
分析证书 | P1508444 |
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