计算溶液所需的质量、体积或浓度。
钙盐染色液（茜素红S法）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
钙在人体内大量存在，构成骨骼作为支持人体的支架，在分泌、运送、肌肉收缩、神经传导等也起重要作用。钙在机体内以两种形式存在，一种是离子钙，存在血液循环内，即所谓血钙；另一种是结合钙，和蛋白、碳酸或磷酸结合而沉着在组织内。除骨骼和牙齿外正常时钙渗透在所有组织和细胞中，一般不以固体状态出现在组织内；但在某些情况下，钙析出成固体并沉着于组织内，则为病理性钙盐沉着。沉着的钙盐主要是磷酸钙，其次为碳酸钙，钙盐通常是单折射的，但草酸钙是双折射的。当使用HE染色时钙一般呈紫蓝色，许多染料可与钙形成螯合物，包括茜素红S、红紫素、核固红等，一般来说这些染料在识别中至大量的钙时，效果优于轻微染色的微量钙沉积，但茜素红S往往对少量的沉积物可得到更可靠的结果。
钙盐染色常用方法有硝酸银法和茜素红S法，本产品采用茜素红S和固绿结合染色，茜素红S属一种蒽醌类衍生物，是茜素磺酸钠盐，它能与碳酸钙或磷酸钙中的钙盐螯合形成橙红色复合物；钙盐和茜素红结合形成橘红色沉淀，适用于少量钙盐组织的染色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
| P1508560 | Component | 2×50mL | Storage |
| P1508560A | 茜素红S染色液 | 50 mL | RT. |
| P1508560B | 固绿染色液 | 50 mL | RT. |
自备仪器与试剂
- 固定液：10%中性福尔马林、乙醇福尔马林和乙醇等
- 系列乙醇、蒸馏水、二甲苯或脱蜡透明液、中性树胶、乙酸水溶液
- 显微镜
使用说明（仅供参考）
1、常规染色
- 组织固定于10%中性福尔马林或乙醇，常规脱水包埋。
- 切片至95%乙醇，载玻片竖立放置，彻底风干。
- 切片入含茜素红S染色液的玻片染缸，浸染1~5min（见注意事项1），蒸馏水快速冲洗。
- 入固绿染色液复染1min，蒸馏水冲洗3次。
- 常规脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
2、不溶性钙染色
- 组织固定于10%中性福尔马林固定液，常规脱水，其中注意在95%乙醇中脱水应充分。
- 塑料包埋，室温过夜，37℃烤箱中聚合2~4天，-20℃冰箱冷却15~20min。
- 切片厚度3~5μm，未脱钙骨切片脱塑至水，蒸馏水洗。
- 切片入茜素红S染色液5~10min(见注意事项2)，蒸馏水洗。
- 入固绿染色液复染30~60s。
- 0.2~1%乙酸水溶液迅速冲洗，流水稍洗。
- 滤纸吸干，95%乙醇脱色。
- 常规脱水，二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果
| 钙沉积物 | 橙红色 |
| 背景 | 绿色 |
注意事项
1、茜素红S的染色时间要根据钙盐的含量来确定，应在显微镜下观察，见钙盐呈较深的橙红色即取出水洗，如染色时间过长就出现弥散现象，一般1~2min即可。
2、在不溶性钙染色中茜素红S的染色时间要根据钙盐的含量来确定，应在显微镜下观察，直到着色反应强烈而不弥散为止。
3、经过茜素红S染色液染色后，钙沉积物是双折射的。
4、该方法在辨别和检测少量钙时特别有用，如肾中的异常钙化（尿钙过多），其显示为橘红色。
5、试剂开封后请尽快使用，以防影响后续实验效果。
Calcium is abundant in the human body, forming the skeleton that provides structural support and playing important roles in secretion, transport, muscle contraction, and nerve conduction. Calcium exists in two forms within the body: one is ionic calcium, present in the bloodstream (referred to as blood calcium); the other is bound calcium, which combines with proteins, carbonates, or phosphates and deposits in tissues. Aside from bones and teeth, calcium normally permeates all tissues and cells and generally does not appear as a solid within tissues. However, under certain conditions, calcium may precipitate as a solid and deposit in tissues, leading to pathological calcification. The deposited calcium salts are primarily calcium phosphate, followed by calcium carbonate. Calcium salts are usually monorefringent, but calcium oxalate is birefringent. When stained with HE, calcium typically appears purplish‑blue. Many dyes can form chelates with calcium, including Alizarin Red S, purpurin, nuclear fast red, etc. Generally, these dyes are more effective in identifying moderate to large amounts of calcium than in staining trace calcium deposits lightly, but Alizarin Red S often yields more reliable results for small amounts of deposits.
Common methods for calcium salt staining include the silver nitrate method and the Alizarin Red S method. This product uses a combined staining method with Alizarin Red S and Fast Green FCF. Alizarin Red S is an anthraquinone derivative and the sodium salt of alizarinsulfonic acid, which can chelate with calcium salts in calcium carbonate or calcium phosphate to form an orange‑red complex; the combination of calcium salts and Alizarin Red produces an orange‑red precipitate, suitable for staining tissues with small amounts of calcium salts. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
| P1508560 | Component | 2×50mL | Storage |
| P1508560A | Alizarin Red S Staining Solution | 50 mL | RT. |
| P1508560B | Fast Green FCF Staining Solution | 50 mL | RT. |
Reagents, consumables and Equipments not provided
- Fixatives: 10% neutral formalin, ethanol‑formalin, ethanol, etc.
- Graded ethanol series, distilled water, xylene or dewaxing clearing solution, neutral balsam, acetic acid aqueous solution
- Microscope
Instructions for Use (For Reference Only)
1. Routine Staining
Fix tissue in 10% neutral formalin or ethanol, followed by routine dehydration and embedding.
Section to 95% ethanol; place slides upright and allow to dry completely.
Immerse sections in a staining jar containing Alizarin Red S Staining Solution for 1–5 minutes (see Note 1); rinse quickly with distilled water.
Counterstain with Fast Green FCF Staining Solution for 1 minute; rinse with distilled water three times.
Routine dehydration, clear with xylene or dewaxing clearing solution, and mount with neutral balsam.
2. Insoluble Calcium Staining
Fix tissue in 10% neutral formalin fixative and dehydrate routinely, ensuring thorough dehydration in 95% ethanol.
Embed in plastic and leave overnight at room temperature; polymerize in a 37°C oven for 2–4 days; cool in a –20°C freezer for 15–20 minutes.
Section to a thickness of 3–5 μm; for undecalcified bone sections, remove plastic and bring to water; wash with distilled water.
Immerse sections in Alizarin Red S Staining Solution for 5–10 minutes (see Note 2); wash with distilled water.
Counterstain with Fast Green FCF Staining Solution for 30–60 seconds.
Rinse quickly with 0.2–1% acetic acid aqueous solution; wash briefly under running water.
Blot dry with filter paper; decolorize with 95% ethanol.
Routine dehydration, clear with xylene or dewaxing clearing solution, and mount with neutral balsam.
Staining Results
| Calcium deposits | Orange‑red |
| Background | Green |
Notes
The staining time with Alizarin Red S should be determined based on the calcium salt content. Observe under a microscope and remove the slide for washing when calcium salts appear a deep orange‑red. Over‑staining may cause diffusion; generally, 1–2 minutes is sufficient.
For insoluble calcium staining, the staining time with Alizarin Red S should be determined based on the calcium salt content. Observe under a microscope until the staining reaction is strong without diffusion.
After staining with Alizarin Red S, calcium deposits are birefringent.
This method is particularly useful for identifying and detecting small amounts of calcium, such as abnormal calcification in the kidneys (hypercalciuria), which appears orange‑red.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
钙盐染色试剂盒
英文别名
Calcium Stain Kit(Alizarin Red S Method)
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months).
英文名称
Calcium Staining Solution (Alizarin Red S Method)
储存条件
室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0332880 |
分析证书 | P1508560 |
技术文档和文章
Identification of Tissue Components, Method Selection, and Result Interpretation in Connective Tissue Staining
目录： 技术文章
Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy
目录： 技术文章
Preparation Strategy and In Vitro Biological Performance Evaluation of Tannic Acid–Brushite In Situ Composite Bone Cement
目录： 技术文章
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