计算溶液所需的质量、体积或浓度。
青霉素-链霉素-新霉素溶液 (100×, 无菌)
有货
为什么选择此级别
BioReagent,无菌过滤,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
在体外培养中为预防微生物污染，常在培养液中加入一定量抗生素。其中，以青霉素的使用最为广泛。青霉素能够干扰细菌细胞壁的合成，对革兰阳性菌特别有效；链霉素能够与细菌核糖体30S亚单位结合，抑制细菌蛋白质的合成，对革兰氏阴性菌和革兰氏阳性菌均有效，但对革兰氏阴性菌特别有效；而新霉素则能够结合到细菌核糖体30S和50S亚单位引起蛋白错误编码，在蛋白合成起间抑制起始和延长，对革兰氏阴性菌和革兰氏阳性菌均有效，尤其是对金葡菌、白喉杆菌和炭疽杆菌作用最好。但青霉素溶液对温度和酸碱度较为敏感，室温易降解，pH 6.0-6.5时最为稳定；链霉素则相对稳定，pH 5.0-7.5时最为稳定；而硫酸新霉素则较为稳定。
青霉素-链霉素-新霉素溶液 (100×, 无菌) (Penicillin-Streptomycin-Neomycin, PSN)是经过滤除菌的无菌溶液，可直接用于细胞培养的三抗溶液。本品中含有青霉素G钠盐5 KU/mL，硫酸链霉素5 mg/mL，硫酸新霉素10 mg/mL，由0.85% NaCl溶液配制而成。推荐工作浓度：青霉素为50 U/mL，链霉素的为50 μg/mL，新霉素为100 μg/mL，即按照100倍稀释本品即可。青霉素-链霉素-新霉素溶液联合使用可以大大增强防污染的能力，对革兰阳性细菌和革兰阴性细菌具有有效的联合抗菌作用，可用于防止细胞培养受到细菌污染。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤 (仅供参考)：
青霉素-链霉素-新霉素溶液 (100×, 无菌)可以参考如下两种方法之一使用：
1、在无菌的细胞培养液中按比例1:100 (v/v) 直接添加青霉素-链霉素-新霉素溶液 (PSN) (100×)，混匀即可使用。
2、配制细胞培养液时按比例1:100 (v/v) 加入青霉素-链霉素-新霉素溶液 (PSN) (100×)，配制完成后过滤除菌即可使用。
注意事项：
1、尽量减少反复冻融的次数，以免效率下降。
2、试剂开封后请尽快使用，以防影响后续实验效果。
3、注意无菌操作，避免污染。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
To prevent microbial contamination in in vitro culture, a certain amount of antibiotics is often added to the culture medium. Among these antibiotics, penicillin is the most widely used. Penicillin can interfere with the synthesis of bacterial cell walls and is particularly effective against Gram-positive bacteria. Streptomycin can bind to the 30S subunit of bacterial ribosomes to inhibit bacterial protein synthesis; it is effective against both Gram-negative and Gram-positive bacteria, with particular efficacy against Gram-negative bacteria. Neomycin can bind to the 30S and 50S subunits of bacterial ribosomes, causing misencoding of proteins, inhibiting the initiation and elongation stages of protein synthesis. It is effective against both Gram-negative and Gram-positive bacteria, and exhibits the strongest activity especially against Staphylococcus aureus, Corynebacterium diphtheriae and Bacillus anthracis.
However, penicillin solution is relatively sensitive to temperature and pH, prone to degradation at room temperature, and most stable at pH 6.0-6.5. Streptomycin is relatively stable, with optimal stability at pH 5.0-7.5, while neomycin sulfate is comparatively stable.
Penicillin-Streptomycin-Neomycin Solution (100×) (PSN) is a sterile solution sterilized by filtration, which can be directly used as a triple-antibiotic solution for cell culture. This product contains 5 KU/mL of penicillin G sodium salt, 5 mg/mL of streptomycin sulfate and 10 mg/mL of neomycin sulfate, formulated in 0.85% NaCl solution. The recommended working concentration is 50 U/mL for penicillin, 50 μg/mL for streptomycin and 100 μg/mL for neomycin, which can be achieved by diluting this product 100-fold. The combined use of Penicillin-Streptomycin-Neomycin Solution can greatly enhance anti-contamination capacity, exerting effective synergistic antibacterial effects against both Gram-positive and Gram-negative bacteria, and can be used to prevent bacterial contamination in cell culture. This reagent is for research use only and is not suitable for clinical diagnosis or any other purposes.
Operating Procedures (for reference only)
Penicillin-Streptomycin-Neomycin Solution (100×, Sterile) can be used by either of the following two methods:
1. Add Penicillin-Streptomycin-Neomycin Solution (PSN) (100×) directly to the sterile cell culture medium at a volume ratio of 1:100 (v/v), and mix well before use.
2. Add Penicillin-Streptomycin-Neomycin Solution (PSN) (100×) at a volume ratio of 1:100 (v/v) during the preparation of cell culture medium. Sterilize the prepared medium by filtration before use.
Precautions
1. Minimize the number of freeze-thaw cycles to avoid activity reduction.
2. Please use the reagent as soon as possible after opening to prevent adverse effects on subsequent experimental results.
3. Follow strict aseptic technique to avoid contamination.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
青霉素-链霉素-新霉素溶液 | PSN溶液
英文别名
Penicillin-Streptomycin-Neomycin Solution | PSN Solution
规格或纯度
无菌过滤,BioReagent,用于细胞培养,100×
稳定性与储存
Store at -20℃ long term (12 months). Store in the dark.
英文名称
Penicillin-Streptomycin-Neomycin Solution (100×, Sterile)
储存条件
避光,-20°C储存
运输条件
超低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434137 |
分析证书 | P1509610 |
技术文档和文章
溶液计算器
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