计算溶液所需的质量、体积或浓度。
植物粘胶类物质染色液（铁盐吸收法）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
一些十字花科（如拟南芥, Arabidopsis thaliana）和车前科植物的种皮外层细胞，在发育过程中会合成并分泌大量粘液质多糖。该多糖具有遇水膨胀释放的特性，释放后可形成透明胶状物质包裹在种子周围。
种皮粘液质的主要成分为果胶质（核心为鼠李半乳糖醛酸聚糖），同时还含有少量纤维素和半纤维素。作为一种特化的细胞壁结构，种皮粘液质具备表型易观察、分离提取简便、组成相对单一，且缺失后不影响植株正常生长发育等显著优点，已成为研究植物细胞壁（尤其是果胶）多糖合成、调控机制及细胞壁组分间互作的理想模式体系。
上述提及的粘液质，也属于粘胶类物质的范畴。粘胶类物质又称粘液质，包含多种类似化合物，其中最主要的是粘胶质和原粘胶质，二者的共同特点是水解后可产生粘胶质酸。从溶解特性来看，粘胶质可溶于冷水，而原粘胶质不溶于冷水；从分布范围来看，原粘胶质仅存在于细胞壁中，粘胶质则既存在于细胞壁，也可存在于植物浸出液中。植物细胞的胞间层中含有大量粘胶类物质，这一特性可通过钌红染色法和铁盐吸收法进行验证。
植物粘胶类物质染色液（铁盐吸收法）的染色原理：基于粘胶类物质易吸收铁盐的特性，借助普鲁士蓝染色反应，可区分未被破坏和已被破坏的细胞壁——未被破坏的细胞壁会被染成蓝色，已被破坏的细胞壁则无法着色或着色很浅。需特别说明的是，该产品仅用于科研领域，不适用于临床诊断或其他用途。
自备材料
- 显微镜、镊子、吸管、恒温箱或水浴锅
- 蒸馏水
- 拟南芥种子、植物组织（正常组织或病害组织）等样品
操作步骤（仅供参考）
1. 植物组织粘胶质的染色
1.1 将植物组织切成薄片，如果需要观察病菌分解粘胶类物质的作用，则切片应包括病害部分和正常部分。
1.2 切片滴加铁盐溶液处理10~30 min。
1.3 蒸馏水洗3~5次，每次2 min，充分洗去未吸收的铁盐。
1.4 切片上滴加1~2滴Perls Solution A，处理2 min后再滴加等体积的Perls Solution B，混合染色15~30 min。
1.5 蒸馏水洗2~5 min。
1.6 滴加甘油或甘油明胶封片剂，盖盖玻片，显微镜镜检。
2. 种子粘胶质的染色
2.1 将一定数量的成熟种子放入离心管或平皿中，加入适当体积的蒸馏水，湿润种子，并使其充分吸收水分，根据种子大小，时间为1~12 h不等。
2.2 在不扰动种子的情况下尽可能多地吸除水分。
2.3 染色步骤参考组织的染色步骤（1.2 ~ 1.5）。
2.4 将种子转移到凹槽载玻片上观察染色情况。
实验结果
植物病虫害组织：
植物种子：
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注意事项
1. Perls Solution A易避光保存，亦不可提前与Perls Solution B混合使用。
2. 由于干扰物质的影响，不染色并不代表不存在粘胶质。
3. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
4. 试剂开封后请尽快使用，以防影响后续实验效果。
Some Brassicaceae (such as Arabidopsis thaliana) and Plantaginaceae plants synthesize and secrete large amounts of mucilaginous polysaccharides in the outer layer of their seed coat cells during development. This polysaccharide has the property of swelling and releasing upon contact with water, forming a transparent gelatinous substance that envelopes the seed.
The main component of seed coat mucilage is pectin (with rhamnogalacturonan as the core), along with small amounts of cellulose and hemicellulose. As a specialized cell wall structure, seed coat mucilage offers significant advantages such as easily observable phenotypes, simple isolation and extraction, relatively simple composition, and no adverse effects on normal plant growth and development when absent. It has thus become an ideal model system for studying plant cell wall (especially pectin) polysaccharide synthesis, regulatory mechanisms, and interactions among cell wall components.
The aforementioned mucilage also falls within the category of mucilaginous substances. Mucilaginous substances, also known as mucilage, encompass various similar compounds, the most important of which are mucilage and protopectin. Both share the characteristic of producing mucic acid upon hydrolysis. In terms of solubility, mucilage is soluble in cold water, whereas protopectin is insoluble in cold water. Regarding distribution, protopectin exists only in cell walls, while mucilage can be found both in cell walls and in plant extracts. The middle lamella of plant cells contains large amounts of mucilaginous substances, a property that can be verified using ruthenium red staining and iron salt absorption methods.
Staining Principle of Plant Mucilaginous Substances Staining Solution (Iron Salt Absorption Method): Based on the property of mucilaginous substances to readily absorb iron salts, the Prussian blue staining reaction can distinguish between intact and damaged cell walls—intact cell walls are stained blue, while damaged cell walls either remain unstained or are lightly stained. It is important to note that this product is intended for research purposes only and is not suitable for clinical diagnosis or other uses.
Materials to Be Prepared by User
- Microscope, forceps, pipette, incubator or water bath
- Distilled water
- Samples such as Arabidopsis seeds, plant tissues (normal or diseased tissues), etc.
Procedure (For Reference Only)
1. Staining of Mucilage in Plant Tissues
1.1 Slice the plant tissue into thin sections. If observing the effect of pathogen decomposition on mucilaginous substances, include both diseased and normal parts in the slice.
1.2 Add Iron Salt Solution dropwise onto the slice and treat for 10–30 minutes.
1.3 Rinse 3–5 times with distilled water, 2 minutes each time, to thoroughly wash away unabsorbed iron salts.
1.4 Add 1–2 drops of Perls Solution A onto the slice. After 2 minutes, add an equal volume of Perls Solution B. Mix and stain for 15–30 minutes.
1.5 Rinse with distilled water for 2–5 minutes.
1.6 Add glycerol or glycerol-gelatin mounting medium, cover with a coverslip, and examine under a microscope.
2. Staining of Seed Mucilage
2.1 Place a certain number of mature seeds into a centrifuge tube or Petri dish. Add an appropriate volume of distilled water to moisten the seeds and allow them to fully absorb water. The duration varies from 1 to 12 hours depending on seed size.
2.2 Remove as much water as possible without disturbing the seeds.
2.3 Refer to the tissue staining procedure (steps 1.2–1.5) for the staining steps.
2.4 Transfer the seeds to a concave slide to observe the staining results.
Experimental Results
Plant Diseased and Healthy Tissues:
Plant Seeds:
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Notes
1. Perls Solution A should be stored protected from light and should not be pre-mixed with Perls Solution B.
2. Lack of staining does not necessarily indicate the absence of mucilage due to potential interference from other substances.
3. For your safety and health, please wear a lab coat and disposable gloves during operation.
4. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
规格
英文别名
Mucilage Staining Solution (Iron Salt Method)
规格或纯度
BioReagent,生物染色剂,用于显微镜
英文名称
Plant Mucilage Staining Solution (Iron Salt Absorption Method)
敏感性
Light-sensitive
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
组分与储存
| P1511380 | Component | 3×50 mL | Storage |
| P1511380A | 铁盐溶液 | 50 mL | RT. |
| P1511380B | Perls Solution A | 50 mL | RT. Store in the dark. |
| P1511380C | Perls Solution B | 50 mL | RT. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434171 |
分析证书 | P1511380 |
技术文档和文章
溶液计算器
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