计算溶液所需的质量、体积或浓度。
果胶染色液（羟铵法）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
果胶类物质以原果胶、果胶（Pectin）、果胶酸的形态广泛存在于植物果实、根、茎、叶中，作为细胞壁组成成分与纤维素伴生，是相邻细胞中间层的粘结物，可使植物组织细胞紧密黏结。其中原果胶为非水溶性物质，在未成熟果实或植物组织中，果胶物质多与纤维素结合以原果胶形式存在，能让植物组织坚硬、变脆，且原果胶可在酸、碱、盐等化学试剂及酶的作用下水解，转化为水溶性果胶；果胶又称多聚半乳糖醛酸，是由 D - 半乳糖醛酸以 α-1,4 糖苷键连接形成的直链状多糖聚合物，含数百至约 1000 个脱水半乳糖醛酸残基，平均相对分子质量为 50000~150000。
本产品基于氯化铁羟胺反应实现染色，染色后果胶呈红色，主要适用于新鲜植物切片、石蜡切片、徒手切片等样本的染色，该试剂仅限科研领域使用，不可用于临床诊断等其他用途。
| P1511526 | Component | 4×50 mL | Storage |
| P1511526A | 酸性增强液 | 50 mL | RT. |
| P1511526B | 羟胺溶液 | 25 mL | RT. |
| P1511526C | 碱性溶液 | 25 mL | RT. Store in the dark. |
| P1511526D | 酸化液 | 50 mL | RT. |
| P1511526E | 氯化铁溶液 | 50 mL | RT. |
临用前，按羟胺溶液：碱性溶液=1:1比例混合，即为碱性羟胺染色液，即配即用。
自备材料
- 载玻片
- 系列乙醇
操作步骤（仅供参考）
1. 切片：选用新鲜组织切片、手工切片、滑走切片机切片等，置于载玻片上。
2. 增强：滴加37～45℃预热酸性增强液覆盖切片，室温放置1-3 min。
3. 弃液，流水稍冲洗。
4. 碱化：滴加250-500 μL碱性羟胺染色液完全覆盖切片，室温放置5 min或更长时间。
5. 酸化：直接滴加等量的酸化液，室温放置2 min。
6. 吸去液体，滤纸吸干，滴加氯化铁溶液浸没切片，室温放置3-8 min。
染色结果
| 果胶 | 红色 |
注意事项
1. 一般石蜡切片效果不佳，切片脱蜡应尽量干净。
2. 避免使用铬酸固定剂，铬酸盐处理也会妨碍铁的保存。
3. 试剂开封后请尽快使用，以防影响后续实验效果。
Pectic substances, existing in the forms of protopectin, pectin, and pectic acid, are widely distributed in plant fruits, roots, stems, and leaves. As a cell wall component associated with cellulose, they act as the adhesive in the middle lamella between adjacent cells, contributing to the tight bonding of plant tissue cells. Among these, protopectin is water-insoluble. In unripe fruits or plant tissues, pectic substances are often bound to cellulose in the form of protopectin, lending rigidity and brittleness to the plant tissue. Protopectin can be hydrolyzed by chemical reagents such as acids, alkalis, salts, or by enzymes, converting into water-soluble pectin. Pectin, also known as polygalacturonic acid, is a linear polysaccharide polymer composed of D-galacturonic acid residues linked by α-1,4 glycosidic bonds. It contains several hundred to about 1000 dehydrated galacturonic acid residues, with an average relative molecular mass ranging from 50,000 to 150,000.
This product is based on the ferric chloride-hydroxylamine reaction for staining. After staining, pectin appears red. It is primarily suitable for staining samples such as fresh plant sections, paraffin sections, and free-hand sections. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.
| P1511526 | Component | 4×50 mL | Storage |
| P1511526A | Acid Enhancer Solution | 50 mL | RT. |
| P1511526B | Hydroxylamine Solution | 25 mL | RT. |
| P1511526C | Alkaline Solution | 25 mL | RT. Store in the dark. |
| P1511526D | Acidification Solution | 50 mL | RT. |
| P1511526E | Ferric Chloride Solution | 50 mL | RT. |
Before use, mix Hydroxylamine Solution and Alkaline Solution at a 1:1 ratio to prepare the Alkaline Hydroxylamine Staining Solution. Prepare fresh for immediate use.
Materials to Be Prepared by User
- Glass slides
- Graded ethanol series
Procedure (For Reference Only)
1. Sectioning: Use fresh tissue sections, free-hand sections, or sections from a sliding microtome, and place them on a glass slide.
2. Enhancement: Add pre-warmed (37–45°C) Acid Enhancer Solution to cover the section. Allow it to stand at room temperature for 1-3 minutes.
3. Discard the solution and rinse briefly under running water.
4. Alkalinization: Add 250-500 μL of Alkaline Hydroxylamine Staining Solution to completely cover the section. Allow it to stand at room temperature for 5 minutes or longer.
5. Acidification: Directly add an equal amount of Acidification Solution. Allow it to stand at room temperature for 2 minutes.
6. Remove the liquid and blot dry with filter paper. Add Ferric Chloride Solution to immerse the section. Allow it to stand at room temperature for 3-8 minutes.
Staining Results
|
Notes
1. Staining results are generally suboptimal for paraffin sections. Ensure paraffin removal is as thorough as possible.
2. Avoid the use of chromic acid fixatives, as chromate treatment can also interfere with iron retention.
3. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
规格
别名
羟胺 - 氯化铁果胶染色液 | 甲基酯化果胶染色液
英文别名
HA-FC pectin stain
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Pectin staining solution (hydroxylamine method)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434145 |
分析证书 | P1511526 |
技术文档和文章
溶液计算器
常见问题
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →相关产品
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