计算溶液所需的质量、体积或浓度。
植物花粉管染色液（苯胺蓝荧光法）
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜,适用于荧光分析 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
有花植物需通过花粉将精细胞运送至胚珠以完成双受精，该过程需克服两大关键障碍：一是花粉自花药释放后抵达雌蕊的传粉过程，两性花依赖花药开裂完成，单性花则需风或昆虫协助；二是花粉附着柱头后，需将精细胞运送至封闭于胚珠内的卵细胞。为此，花粉会萌发花粉管以实现精细胞的定向转运，其生长主要包括花粉与柱头黏附、花粉水合、花粉管萌发与伸长、珠孔导向生长及精细胞释放等阶段。
胼胝质（callose）是葡萄糖残基以非纤维素方式连接形成的多糖，参与植物筛管代谢、配子体发育等重要生命活动，其合成与降解直接影响植物正常生长代谢，是植物研究的重要内容。
植物花粉管染色液（苯胺蓝荧光法）包含苯胺蓝染色液与稀释液，其原理为水溶性苯胺蓝可特异性结合花粉管胼胝质，经紫外光激发后呈现黄绿色荧光。本品操作简便快捷，适用于水稻、拟南芥等植物花粉管生长过程的荧光显微镜观察，仅限科研领域使用，不用于临床诊断及其他非科研用途。
自备材料
- AAF固定液 (50%) 或Carnoy固定液或AF固定液、50%乙醇、无水乙醇、1×PBS、甘油明胶封片剂或抗荧光淬灭封片剂
- 新鲜的植物组织切片（花粉管）
操作步骤（仅供参考，固定和软化方法根据实际情况而定）
1. 配制苯胺蓝染色工作液：将苯胺蓝染色液和苯胺蓝稀释液等比例混合，现配现用。
2. 取新鲜雌蕊在AAF固定液 (50%) 或Carnoy固定液或AF固定液中固定10 h，用蒸馏水冲洗干净。
3. 在45℃水浴中利用2 mol/L NaOH软化2 h~过夜，蒸馏水冲洗3次。
4. 用苯胺蓝染色工作液室温避光染色4 h。
5. 将染色后的雌蕊（纵向包括子房）置载玻片上，滴加25 μL水性甘油明胶封片剂或抗荧光淬灭封片剂，盖上盖玻片，轻轻压平。
6. 在荧光显微镜下观察花柱中的花粉管，紫外光激发，激发波长为330~385 nm (BP)，发射波长为425 nm (LP)。用未经染色的花粉管做对照。
染色结果
花粉管产生的胼胝质在紫外光激发下呈黄绿色荧光，背景无着色。
注意事项
1. 在条件允许下，推荐将浸于固定液中的植物组织负压真空处理20 min，有助于固定液的渗透。
2. 如暂时不进行实验或需同时处理大量样本，浸于100%乙醇的样本可于2-8℃保存至少1周。
3. 样本染色完成后尽快拍照保存结果。
4. 染色液有效成分易分解，建议临用前配置，在3小时内使用。使用过程中可能出现试剂颜色少量变浅，属于正常现象。
5. 荧光染色容易发生光淬灭，在染色和转移以及观察过程中注意避免激发光以外的环境光。
6. 试剂开封后请尽快使用，以防影响后续实验效果。
7. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Flowering plants need to transport sperm cells to the ovule via pollen to complete double fertilization. This process must overcome two key obstacles: first, the pollination process from pollen release from the anther to reaching the pistil, which in bisexual flowers relies on anther dehiscence, while in unisexual flowers requires assistance from wind or insects; second, after pollen adheres to the stigma, sperm cells must be transported to the egg cell enclosed within the ovule. To achieve this, pollen germinates a pollen tube to facilitate the directional transport of sperm cells. Its growth mainly includes stages such as pollen-stigma adhesion, pollen hydration, pollen tube germination and elongation, micropyle-guided growth, and sperm cell release.
Callose is a polysaccharide formed by glucose residues linked in a non-cellulosic manner. It participates in important biological activities such as plant sieve tube metabolism and gametophyte development. Its synthesis and degradation directly affect normal plant growth and metabolism, making it a significant subject in plant research.
Plant Pollen Tube Staining Solution (Aniline Blue Fluorescence Method) contains aniline blue staining solution and a diluent. The principle is based on water-soluble aniline blue specifically binding to callose in pollen tubes, which emits yellow-green fluorescence upon ultraviolet excitation. This product is simple and quick to operate, suitable for fluorescence microscope observation of pollen tube growth in plants such as rice and Arabidopsis. It is for research use only and not intended for clinical diagnosis or other non-research purposes.
Materials to Be Prepared by User
- AAF Fixative Solution (50%) or Carnoy's Fixative Solution or AF Fixative Solution, 50% Ethanol, Absolute Ethanol, 1× PBS, Glycerol-Gelatin Mounting Medium or Anti-Fade Mounting Medium
- Fresh plant tissue sections (pollen tubes)
Procedure (For Reference Only; Fixation and Softening Methods May Vary Depending on Actual Conditions)
Prepare the Aniline Blue Working Solution: Mix the Aniline Blue Staining Solution and Aniline Blue Diluent in equal proportions. Prepare immediately before use.
Take fresh pistils and fix them in AAF Fixative Solution (50%), Carnoy's Fixative Solution, or AF Fixative Solution for 10 hours. Rinse thoroughly with distilled water.
Soften the samples in 2 mol/L NaOH at 45°C in a water bath for 2 hours to overnight. Rinse three times with distilled water.
Stain with the Aniline Blue Working Solution at room temperature, protected from light, for 4 hours.
Place the stained pistils (longitudinally including the ovary) on a glass slide. Add 25 μL of aqueous Glycerol-Gelatin Mounting Medium or Anti-Fade Mounting Medium, cover with a coverslip, and gently flatten.
Observe the pollen tubes in the style under a fluorescence microscope using UV excitation light (excitation wavelength 330~385 nm (BP), emission wavelength 425 nm (LP)). Use unstained pollen tubes as a control.
Staining Results
Callose produced by pollen tubes emits yellow-green fluorescence under UV excitation, with no background staining.
Notes
When conditions permit, it is recommended to subject the plant tissue immersed in fixative to negative pressure/vacuum treatment for 20 minutes to aid fixative penetration.
If the experiment is not to be performed immediately or a large number of samples need to be processed simultaneously, samples stored in 100% ethanol can be kept at 2-8°C for at least one week.
Photograph the stained samples as soon as possible after staining to preserve the results.
The active components of the staining solution are prone to decomposition. It is recommended to prepare the working solution immediately before use and use it within 3 hours. A slight color fading of the reagent during use is a normal phenomenon.
Fluorescent staining is susceptible to photobleaching. Take care to avoid ambient light other than the excitation light during staining, transfer, and observation.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
For your safety and health, please wear a lab coat and disposable gloves during operation.
规格
别名
苯胺蓝荧光花粉管染色液 | 花粉管胼胝质荧光染色液 | 水溶性苯胺蓝花粉管染色液 | 植物花粉管苯胺蓝荧光染色试剂 | 花粉管特异性荧光染色液（苯胺蓝法）
英文别名
Aniline Blue Fluorescent Stain for Plant Pollen Tubes | Water-Soluble Aniline Blue Pollen Tube Staining Solution | Pollen Tube Callose Fluorescence Stain (Aniline Blue) | Aniline Blue Fluorescence Assay for Pollen Tube Visualization | Plant Pollen Tube De
规格或纯度
BioReagent,生物染色剂,用于显微镜,适用于荧光分析
英文名称
Plant Pollen Tube Staining Solution (Aniline Blue Fluorescence Method)
敏感性
Light-sensitive
Emission(nm)
425nm
Excitation(nm)
330-385nm
Ex/Em(nm)
330-385/425nm
储存与运输
储存条件
避光,室温
运输条件
常规运输
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
组分与储存
| P1511521 | Component | 2×25 mL | Storage |
| P1511521A | 苯胺蓝染色液 | 25 mL | RT. Store in the dark. |
| P1511521B | 苯胺蓝稀释液 | 25 mL | RT. Store in the dark. |
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434174 |
分析证书 | P1511521 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Fluorescence analysis 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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