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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
果胶裂解酶(EC4.2.2.10)是果胶酶的重要组成部分,是一种能降解植物细胞壁,导致植物组织软化甚至死亡的解聚酶,来源比较广泛,主要来源于微生物,可用于果汁、果酒的澄清,提高水果出汁率,植物病毒的纯化,纸浆的漂白和纺织品的生物精炼,在减少环境污染和降低能源消耗方面具有潜在的应用价值。该产品提供了一种简单、方便、快速的 PL 活性检测方法,适用于植物组织、细菌、真菌和培养液等样本。其原理是果胶裂解酶作用于果胶中的α-1,4 糖苷键,生成在还原端 C4 和 C5 之间位置具有不饱和键的不饱和寡聚半乳糖醛酸,在235 nm处有特征吸收峰。
Pectin Lyase (EC 4.2.2.10) is an important component of pectinase enzymes. It is a depolymerizing enzyme that degrades plant cell walls, leading to the softening and even death of plant tissues. It has a wide range of sources, primarily derived from microorganisms. It can be used for the clarification of fruit juices and wines, improving the juice yield of fruits, the purification of plant viruses, the bleaching of paper pulp, and the bio-scouring of textiles. It holds potential application value in reducing environmental pollution and lowering energy consumption. This product provides a simple, convenient, and rapid method for assaying PL activity, suitable for samples such as plant tissues, bacteria, fungi, and culture medium. Its principle is that pectin lyase acts on the α-1,4 glycosidic bonds in pectin, generating unsaturated oligogalacturonides with an unsaturated bond between the C4 and C5 positions at the reducing end, which has a characteristic absorption peak at 235 nm.